Ex vivo culture of pre-placental tissues reveals that the allantois is required for maintained expression of Gcm1 and Tpbpα

Hou, Wenyang; Sarikaya, Didem P.; Jerome-Majewska, Loydie A.

doi:10.1016/j.placenta.2016.08.091

Cited by 4 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cutting and manipulating the allantois may damage the chorioadhesive cells in the outer sheath of the allantoic mesothelium. Nevertheless, cutting the allantois with forceps has been successfully used before in ex vivo cultures of pre-placental tissues 17 , and the kinetics of allantoic explant attachment and the morphology of the explant cultures described in the present study are similar to published data based on allantois isolation by aspiration 16 . These findings suggest that chorioadhesive cells are sufficiently well preserved upon manual cutting of the allantois, and that explant differentiation is not grossly perturbed, as indicated by the formation of CD31-positive endothelia.…”

Section: Discussionsupporting

confidence: 83%

Dissection and Explant Culture of Murine Allantois for the <em>In Vitro</em> Analysis of Allantoic Attachment

Hadamek

Keller

Gohla

2018

JoVE

View full text Add to dashboard Cite

The placenta is essential for the growth and development of mammalian embryos. For this reason, numerous genetic alterations and likely also environmental insults that disturb placenta development or function can cause early pregnancy loss in mice and humans. Nevertheless, simple in vitro assays to screen for potential effects on placenta formation are lacking. Here, we focus on modeling the first and critical step in placenta formation, which consists of the attachment of the allantois to the chorion. We describe a method to rapidly assess the attachment of allantoic explants on immobilized α4β1 integrin, which serves as a chorio-mimetic substrate.This in vitro approach enables a qualitative evaluation of the attachment and spreading behavior of multiple allantois explants at different consecutive time points. The protocol may be used to investigate the effect of targeted mouse mutations, drugs, or various environmental factors that have been linked to pregnancy complications or fetal loss on allantois attachment ex vivo.

show abstract

Section: Discussionsupporting

confidence: 83%

Dissection and Explant Culture of Murine Allantois for the <em>In Vitro</em> Analysis of Allantoic Attachment

Hadamek

Keller

Gohla

2018

JoVE

View full text Add to dashboard Cite

show abstract

“…Liver samples fixed in Bouin and/or PFA were dehydrated and embedded in paraffin, as previously described [ 5 , 18 , 19 ]. All samples were sectioned at 5μm thickness.…”

Section: Methodsmentioning

confidence: 99%

Non-alcoholic fatty liver disease in mice with heterozygous mutation in TMED2

et al. 2017

Self Cite

View full text Add to dashboard Cite

The transmembrane emp24 domain/p24 (TMED) family are essential components of the vesicular transport machinery. Members of the TMED family serve as cargo receptors implicated in selection and packaging of endoplasmic reticulum (ER) luminal proteins into coatomer (COP) II coated vesicles for anterograde transport to the Golgi. Deletion or mutations of Tmed genes in yeast and Drosophila results in ER-stress and activation of the unfolded protein response (UPR). The UPR leads to expression of genes and proteins important for expanding the folding capacity of the ER, degrading misfolded proteins, and reducing the load of new proteins entering the ER. The UPR is activated in non-alcoholic fatty liver disease (NAFLD) in human and mouse and may contribute to the development and the progression of NAFLD. Tmed2, the sole member of the vertebrate Tmed β subfamily, exhibits tissue and temporal specific patterns of expression in embryos and developing placenta but is ubiquitously expressed in all adult organs. We previously identified a single point mutation, the 99J mutation, in the signal sequence of Tmed2 in an N-ethyl-N-nitrosourea (ENU) mutagenesis screen. Histological and molecular analysis of livers from heterozygous mice carrying the 99J mutation, Tmed299J/+, revealed a requirement for TMED2 in liver health. We show that Tmed299J/+ mice had decreased levels of TMED2 and TMED10, dilated endoplasmic reticulum membrane, and increased phosphorylation of eIF2α, indicating ER-stress and activation of the UPR. Increased expression of Srebp1a and 2 at the newborn stage and increased incidence of NAFLD were also found in Tmed299J/+ mice. Our data establishes Tmed299J/+ mice as a novel mouse model for NAFLD and supports a role for TMED2 in liver health.

show abstract

“…Various ex vivo methods to study the allantois are available ( Downs, 2006 ; Arora and Papaioannou, 2012 ; Hou et al, 2016 ; Hadamek et al, 2018 ). However, they do not readily provide a means to incorporate hemodynamic factors ( Arora and Papaioannou, 2012 ).…”

Section: Mechanotransduction In the Murine Vitelline And Umbilico-pla...mentioning

confidence: 99%

“…The allantois is the precursor for the umbilical cord in mouse ( Inman and Downs, 2007 ; Arora and Papaioannou, 2012 ) and contributes to the umbilical cord in human ( Spurway et al, 2012 ; Basta and Lipsett, 2023 ). The murine allantois is capable of developing its vasculature independently and can be isolated and cultured ex vivo or transplanted to study vascular development and chorio-allantoic fusion ( Downs and Harmann, 1997 ; Diehl et al, 2001 ; Downs et al, 2001 ; Downs, 2006 ; Arora and Papaioannou, 2012 ; Hou et al, 2016 ; Hadamek et al, 2018 ). In human, the umbilical cord contains one umbilical vein and two umbilical arteries ( Spurway et al, 2012 ; Ramesh et al, 2015 ; Li et al, 2019 ; Ebbing et al, 2020 ), whereas the umbilical cord in mouse has single umbilical artery and vein ( Inman and Downs, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Origin and flow-mediated remodeling of the murine and human extraembryonic circulation systems

Van Schoor,

Bruet,

Jones

et al. 2024

Front. Physiol.

View full text Add to dashboard Cite

The transduction of mechanical stimuli produced by blood flow is an important regulator of vascular development. The vitelline and umbilico-placental circulations are extraembryonic vascular systems that are required for proper embryonic development in mammalian embryos. The morphogenesis of the extraembryonic vasculature and the cardiovascular system of the embryo are hemodynamically and molecularly connected. Here we provide an overview of the establishment of the murine and human vitelline and umbilico-placental vascular systems and how blood flow influences various steps in their development. A deeper comprehension of extraembryonic vessel development may aid the establishment of stem-cell based embryo models and provide novel insights to understanding pregnancy complications related to the umbilical cord and placenta.

show abstract

Ex vivo culture of pre-placental tissues reveals that the allantois is required for maintained expression of Gcm1 and Tpbpα

Cited by 4 publications

References 37 publications

Dissection and Explant Culture of Murine Allantois for the <em>In Vitro</em> Analysis of Allantoic Attachment

Dissection and Explant Culture of Murine Allantois for the <em>In Vitro</em> Analysis of Allantoic Attachment

Non-alcoholic fatty liver disease in mice with heterozygous mutation in TMED2

Origin and flow-mediated remodeling of the murine and human extraembryonic circulation systems

Contact Info

Product

Resources

About