Ex vivo imaging of human pathologies with integrated optical coherence tomography (OCT) and optical coherence microscopy (OCM)

Zhou, Chao; Aguirre, Aaron D.; Wang, Yihong; Bryan, Bradley; Tsai, Tsung Han; Connolly, James L.; Fujimoto, James G.

doi:10.1117/12.809064

Cited by 3 publications

(5 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using the integrated OCT/OCM system, a wide range of human pathological specimens were imaged, including colon (58), thyroid (43), breast (34), and kidney (19). The imaging results were compared with histopathology and representative OCT/OCM images of human thyroid, breast and kidney are demonstrated in this proceeding.…”

Section: Resultsmentioning

confidence: 99%

“…Various methods are implemented to detect and screen for malignant thyroid nodules. To investigate the feasibility of using the integrated OCT/OCM system in differentiating benign and malignant thyroid nodules, ex vivo imaging of fresh excised thyroid tissue was performed [19]. Images of both normal and diseased thyroid specimens were compared with histological slides.…”

Section: Integrated Oct/ocm Imaging Of Human Thyroidmentioning

confidence: 99%

See 1 more Smart Citation

Integrated optical coherence tomography and optical coherence microscopy imaging of human pathology

et al. 2010

Self Cite

View full text Add to dashboard Cite

Excisional biopsy is the current gold standard for disease diagnosis; however, it requires a relatively long processing time and it may also suffer from unacceptable false negative rates due to sampling errors. Optical coherence tomography (OCT) is a promising imaging technique that provide real-time, high resolution and three-dimensional (3D) images of tissue morphology. Optical coherence microscopy (OCM) is an extension of OCT, combining both the coherence gating and the confocal gating techniques. OCM imaging achieves cellular resolution with deeper imaging depth compared to confocal microscopy. An integrated OCT/OCM imaging system can provide co-registered multiscale imaging of tissue morphology. 3D-OCT provides architectural information with a large field of view and can be used to find regions of interest; while OCM provides high magnification to enable cellular imaging. The integrated OCT/OCM system has an axial resolution of <4um and transverse resolutions of 14um and <2um for OCT and OCM, respectively. In this study, a wide range of human pathologic specimens, including colon (58), thyroid (43), breast (34), and kidney (19), were imaged with OCT and OCM within 2 to 6 hours after excision. The images were compared with H & E histology to identify characteristic features useful for disease diagnosis. The feasibility of visualizing human pathology using integrated OCT/OCM was demonstrated in the pathology laboratory settings.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Integrated Oct/ocm Imaging Of Human Thyroidmentioning

confidence: 99%

Integrated optical coherence tomography and optical coherence microscopy imaging of human pathology

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, we explore the feasibility of using integrated 3D-OCT and cellular resolution OCM imaging for multiscale assessment of human thyroid pathologies ex vivo in a pathology laboratory setting. These results have been recently accepted for publication in the Journal of Biomedical Optics [17].…”

Section: Introductionmentioning

confidence: 90%

“…Figures 2 to 4 present examples of data from this study. Representative images from a large spectrum of diseases can be found in reference [17]. En face slices of OCT images (3 x 1.5 mm 2 ) were reconstructed from the 3D data sets by averaging over 10 um intervals in the axial direction to reduce speckle.…”

Section: Methodsmentioning

confidence: 99%

Multiscale imaging of human thyroid pathologies using integrated optical coherence tomography (OCT) and optical coherence microscopy (OCM)

et al. 2010

Self Cite

View full text Add to dashboard Cite

We evaluate the feasibility of optical coherence tomography (OCT) and optical coherence microscopy (OCM) for imaging of benign and malignant thyroid lesions ex vivo using intrinsic optical contrast. Thirty four thyroid gland specimens were imaged from 17 patients, covering a spectrum of pathology, ranging from normal thyroid to neoplasia and benign disease. The integrated OCT and OCM imaging system allows seamlessly switching between low and high magnifications, in a way similar to traditional microscopy. Good correspondence was observed between optical images and histological sections. The results provide a basis for interpretation of future OCT and OCM images of the thyroid tissues and suggest the possibility of future in vivo evaluation of thyroid pathology.

show abstract

“…During the early disease stages, the change in tissue scattering properties between normal and diseased tissue is small and difficult to measure. OCT has been widely used for imaging of human pathologies [32,71,[81][82][83][84][85][86][87][88][89][90][91][92][93][94][95][96][97], where the utility of OCT for early cancer detection in epithelial tissue were investigated. One of the greatest challenges for extending the clinical applications of OCT is to identify more contrast mechanisms that can provide physiological information in addition to morphological structure [36].…”

Section: Optical Coherence Tomographymentioning

confidence: 99%

Investigation of the spectroscopic optical coherence tomography technique for tissue analysis

Tay¹

View full text Add to dashboard Cite

Optical coherence tomography (OCT), a low coherence interferometric imaging technique, is a potentially useful tool for disease diagnosis and biological process monitoring. Spectral analysis of the OCT signals can yield additional information on the properties of scatterers in the sample, allowing tissue analysis and functional imaging to be performed. The objective of this work is to investigate the use of the spectroscopic OCT (SOCT) technique for biomedical applications. A systematic study was first carried out to determine an optimal metric for quantifying scatterer sizes using an improved SOCT analysis method.

show abstract

Ex vivo imaging of human pathologies with integrated optical coherence tomography (OCT) and optical coherence microscopy (OCM)

Cited by 3 publications

References 22 publications

Integrated optical coherence tomography and optical coherence microscopy imaging of human pathology

Integrated optical coherence tomography and optical coherence microscopy imaging of human pathology

Multiscale imaging of human thyroid pathologies using integrated optical coherence tomography (OCT) and optical coherence microscopy (OCM)

Investigation of the spectroscopic optical coherence tomography technique for tissue analysis

Contact Info

Product

Resources

About