Ex Vivo Induction of Apoptosis in Lymphocytes Is Mediated by Oxidative Stress: Role for Lymphocyte Loss in HIV Infection

Dobmeyer, Thomas S.; Findhammer, Stephan; Dobmeyer, Jürgen; Klein, Stefan; Raffel, Bernhard; Hoelzer, Dieter; Helm, E. B.; Kabelitz, Dieter; Rossol, Rita

doi:10.1016/s0891-5849(96)00403-0

Cited by 107 publications

(72 citation statements)

References 38 publications

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“…The trace element appears to be important in reducing the virulence of HIV and slowing the progression of HIV-related disease (Rayman, 2000). It is worth noting that, deficiencies in antioxidants during HIV infection were shown to induce chronic oxidative stress (Pace and Leaf, 1995), which has been linked to apoptosis of T lymphocytes during HIV disease (Dobmeyer et al, 1997) and increased rates of HIV replication by activating the nuclear factor-kB (NF-kB) gene (Schreck et al, 1991). Selenium supplementation stimulates glutathione peroxidase activity and reduces NF-kB activation in HIV-1-infected cell lines (Sappey et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Alterations in serum levels of trace elements in tuberculosis and HIV infections

et al. 2005

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Objective: To evaluate serum concentrations of trace elements in tuberculosis (TB) patients with or with out human immunodeficiency virus (HIV) coinfection before and after anti-TB chemotherapy. Subjects: A total of 155 TB patients, 74 of which were coinfected with HIV, and 31 healthy controls from Gondar, Ethiopia. Methods: Serum levels of copper, zinc, selenium and iron were determined using an inductively coupled plasma mass spectrometer from all subjects at baseline and from 44 TB patients (22 with HIV coinfection) at the end of an intensive phase of anti-TB chemotherapy. Results: Compared with the control group, the concentrations of iron, zinc and selenium were significantly lower (Po0.05) while that of copper and copper/zinc ratio was significantly higher (Po0.05) in the serum of TB patients. TB patients with HIV coinfection had significantly lower serum zinc and selenium concentrations and significantly higher copper/zinc ratio compared to that in TB patients without HIV coinfection (Po0.05). The serum concentration of zinc had significantly increased at the end of intensive phase of anti-TB chemotherapy in patients without HIV coinfection (Po0.05). An increase in serum selenium level was observed in TB patients with or without HIV coinfection after therapy. On the contrary, serum copper concentration and copper/zinc ratio declined significantly after anti-TB chemotherapy irrespective of HIV serostatus (Po0.05). Conclusions:The results indicate that TB patients have altered profile of trace elements in their sera. This warrants the need for further investigations so that strategies for trace elements supplementation can be planned in addition to their potential as diagnostic parameters in monitoring responses to anti-TB chemotherapy.

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Section: Discussionmentioning

confidence: 99%

Alterations in serum levels of trace elements in tuberculosis and HIV infections

et al. 2005

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“…Since the maximum response to TT occurred already after 4 days in culture in HIV þ patients due to increasing apoptosis (Patki et al, 1999), an incubation period of 3 days as performed in our study might have underestimated the proliferative response. Even though oxidative stress is discussed to be responsible for enhanced apoptosis of HIV-infected T lymphocytes in vitro (Sandstrom et al, 1993;Dobmeyer et al, 1997;Israel & Gougerot-Pocidalo, 1997), apoptosis of T cells from HIV þ patients could be reduced after antioxidant supplementation in vitro in some studies (Dobmeyer et al, 1997;Malorni et al, 1998;Li-Weber et al, 2002), but not in all studies (Cayota et al, 1996). We could not find any changes in lymphocyte apoptosis in HIV þ patients by supplementation with beverages rich in polyphenols and antioxidant vitamins despite an increase in plasma antioxidant capacity.…”

Section: Discussionmentioning

confidence: 99%

“…It seems to play a key role in disease progression (Baruchel & Wainberg, 1992), probably by enhancing viral replication and apoptosis-mediated CD4 þ T-cell depletion (Sandstrom et al, 1993;Israel & Gougerot-Pocidalo, 1997). Reactive oxygen species (ROS)-mediated apoptosis is independent of lymphocyte subsets and can already be observed in very early infection stages (Dobmeyer et al, 1997). Oxidative stress in CD4 þ T cells is probably involved in the proliferative defect of these cells in HIV þ patients (Eck et al, 1989;Roederer et al, 1992;Cayota et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Lymphocyte proliferation and apoptosis in HIV-seropositive and healthy subjects during long-term ingestion of fruit juices or a fruit-vegetable-concentrate rich in polyphenols and antioxidant vitamins

et al. 2004

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Objective: We investigated whether ingestion of polyphenols from fruit juices or a fruit-vegetable- concentrate affects lymphocyte proliferation and apoptosis in human immunodeficiency virus (HIV)-seropositive (HIV+) and HIV-seronegative ( HIV-) subjects. Design: Randomized, prospective pilot intervention study. Setting: University of Bonn, Department of General Internal Medicine. Subjects: A total of 23 HIV+ subjects from the HIV outpatient clinic, 18 HIV- controls. Interventions: Subjects ingested either 1 l of fruit juice or 30 ml of fruit-vegetable- concentrate daily for 16 weeks in addition to their regular diet. Lymphocyte proliferation and apoptosis were investigated in peripheral blood mononuclear cells at baseline, during 16-weeks of intervention, and after a 6-week washout. Proliferation was assessed by H-3-thymidine incorporation and apoptosis by nuclear content as measured by flow cytometry. Results: Supplementation of fruit juices increased phytohemagglutinin-induced lymphocyte proliferation ( mitotic index) in HIV+ patients from 18 +/- 16 to 40 +/- 34 (P = 0.004) and in healthy controls from 27 +/- 16 to 51 +/- 21 ( P = 0.016). Apoptosis was not affected in HIV+ patients, but rose in healthy controls from 9710 to 34711 ( apoptotic index; P = 0.001). Intervention with concentrate did not significantly alter proliferation and apoptosis in HIV+ and HIV- subjects. Conclusions: Even though apoptosis did not change in HIV+ subjects, ingestion of polyphenol-rich fruit juices might be favorable to HIV+ patients due to enhanced proliferation, which could restore disturbances in T-cell homeostasis. In healthy controls, increased lymphocyte proliferation during juice consumption was counterbalanced by increased apoptosis

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“…18 ROS generation was responsible for the induction of apoptosis of T lymphocytes obtained from HIV-1-positive patients, and ROS-dependent apoptosis was shown to be independent of cytokines such as TNFa. 22 Some experiments have illustrated that intracellular ROS mediated mouse B-cell activation, proliferation, and differentiation into plasma cells. 20 Whether or not ROS production plays an important role in mediating the functions of IL-22 awaits further comprehensive studies.…”

Section: Il-22-induced Ros Production Neutralized By Il-22 Binding Prmentioning

confidence: 99%

Cloning and characterization of mouse IL-22 binding protein

Wei

Liang

et al. 2003

Genes Immun

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Interleukin-22 (IL-22), a member of IL-10 family, plays some important roles in immune response through activation of the STAT 3 signal transduction pathway. Two types of IL-22-binding receptor have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 may be involved in inflammatory processes specifically regulated by soluble receptors. By screening a mouse genomic library for a human IL-22 binding protein homologue, we identified the mouse genomic clone of IL-22 binding protein. Its coding sequence was verified and isolated by RT-PCR. The gene encodes a protein of 230 amino acids that share 67.1% amino-acid sequence identity with human IL-22 binding protein.We designated this receptor 'mouse IL-22 binding protein' (mIL-22BP). mIL-22BP could be upregulated by LPS stimulation in mouse monocytes. mIL-22BP binds to mouse and human IL-22 and neutralizes STAT3 activation induced by both cytokines in human and rat hepatoma cell lines. Treating B cells with mouse IL-22 induces production of reactive oxygen species, which mIL-22BP blocks.

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Ex Vivo Induction of Apoptosis in Lymphocytes Is Mediated by Oxidative Stress: Role for Lymphocyte Loss in HIV Infection

Cited by 107 publications

References 38 publications

Alterations in serum levels of trace elements in tuberculosis and HIV infections

Alterations in serum levels of trace elements in tuberculosis and HIV infections

Lymphocyte proliferation and apoptosis in HIV-seropositive and healthy subjects during long-term ingestion of fruit juices or a fruit-vegetable-concentrate rich in polyphenols and antioxidant vitamins

Cloning and characterization of mouse IL-22 binding protein

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