Ex vivo investigation of blood monocyte and platelet behaviour in pigs maintained on an atherogenic diet

Krause, S.; Pohl, A.; Pohl, C.; Fink, Sidney; Rühling, K.; Kätzel, R.; Lengerken, G. von; Maak, Steffen; Lösche, Wolfgang; Till, U

doi:10.1016/s0940-2993(11)80150-8

Cited by 5 publications

(6 citation statements)

References 18 publications

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“…Analysis of monocyte functionality is mostly limited to the analysis of such basic monocyte functions as adhesion, migration, phagocytic activity, binding and endocytosis of low density lipoprotein [ 37 - 39 ]. In pathogenesis, however, monocytes encounter complex signals that include soluble factors affecting general monocyte function in circulation and local factors upon monocyte adhesion to the endothelium and migration into the tissue.…”

Section: Diagnosticsmentioning

confidence: 99%

Macrophages in Immunopathology of Atherosclerosis: A Target for Diagnostics and Therapy

Orekhov

Sobenin²,

Gavrilin³

et al. 2015

CPD

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Immunopathology plays important roles in the development of different life-threatening diseases, such as atherosclerosis and its consequences (acute myocardial infarction and stroke), cancer, chronic inflammatory diseases. Effective modulation of the immune system may significantly increase the efficacy of prevention and therapy efforts. Currently there are no marketed drugs capable of normalizing immune system function in an intrinsic and comprehensive way. Here, we describe a test system designed for complex analysis of monocyte activity in individuals to diagnose immunopathology and monitor treatment efficacy. This cell-based test system may also be useful for screening compounds with an immune-correcting effects. Both diagnostic and screening systems are based on primary culture of human monocytes and/or monocyte-derived macrophages. This is the first step in creating a method for assessment of macrophage activity, which is required for further development of immune-correcting drugs. The existing preliminary data provide the basis for realization of this idea.

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Section: Diagnosticsmentioning

confidence: 99%

Macrophages in Immunopathology of Atherosclerosis: A Target for Diagnostics and Therapy

Orekhov

Sobenin²,

Gavrilin³

et al. 2015

CPD

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“…Although already in early 90s it was demonstrated that monocytes obtained from pigs on atherogenic diet show impaired phagocytic capacity (Krause et al 1992). Another study performed on monocytes from hypolipidemic swine showed that lipid metabolism is altered already in circulating monocytes.…”

Section: Monocytes Changes In Atherosclerosismentioning

confidence: 99%

“…Analysis of monocyte function is largely limited to the analysis of basic monocyte functions of freshly isolated cells: adhesion, migration, phagocytic activity, binding and endocytosis of LDL (Krause et al 1992;Mosig et al 2009;Bell and Gerrity 1992). In the pathogenesis of cardiovascular diseases, however, monocytes encounter complex signals that include soluble factors, affecting general monocyte function in circulation and local factors upon monocytes adhesion to the endotheium and migration into the tissue.…”

Section: Monocyte Response To Challenge As a Diagnostic Markermentioning

confidence: 99%

Monocytes as a diagnostic marker of cardiovascular diseases

Gratchev¹,

Sobenin²,

Orekhov³

et al. 2012

Immunobiology

116

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“…Alguns estudos demonstraram que a dislipidemia causa disfunção da parede de pequenos vasos e também no processo inflamatório e imunológico, o que pode levar à produção alterada de resposta a diferentes injúrias (Ishikawa et al,2004). Scardina, et al (2011) (Stokes et al, 2002); e a hiperlipidemia provoca alteração da função dos leucócitos (Krause et al, 1992); e que essas alterações poderiam acelerar a progressão das doenças periodontais. (Stokes et al, 2002;Krause et al, 1992).…”

Section: Doença Periodontal E Dislipidemiaunclassified

“…Scardina, et al (2011) (Stokes et al, 2002); e a hiperlipidemia provoca alteração da função dos leucócitos (Krause et al, 1992); e que essas alterações poderiam acelerar a progressão das doenças periodontais. (Stokes et al, 2002;Krause et al, 1992).…”

Section: Doença Periodontal E Dislipidemiaunclassified

Efeito da dislipidemia sobre a capacidade de defesa - estudo experimental

Paula¹

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Dyslipidemia is characterized by elevated or altered levels of plasma total cholesterol, LDL, HDL and triglycerides. In many cases, dyslipidemia is associated with obesity, although individuals may present altered levels of cholesterol without being obese. Studies have shown an interrelationship between periodontal disease and dyslipidemia. There are, in the literature studies showing a clear relationship between periodontal disease and dyslipidemia, which is also demonstrated in other situations of inflammation and infection. However, although there are suggestions that the inverse relationship can also be true, some limitations observed in the studies did not allow us to ensure that systemic alteration (dyslipidemia) could be influencing the inflammatory response (periodontal disease). Therefore, the objective of this study was to evaluate the behavior of the gingival epithelium and inflammatory response in wistar rats under conditions of experimental dyslipidemia. Dyslipidemia was induced by increasing the amount of cholesterol in the diet of animals, and local inflammation was induced by the application of LPS (E-coli lipopolissacarídeo). We assessed local factors (inflammatory infiltrate in the gingival area), and systemic aspects (inflammatory mediators levels as IL-6, IL-1β and TNF-α) and the plasma levels of proteins involved in the regulation of inflammatory response (SLPI and leptin). In animals with dyslipidemia histopathological analysis showed an increased level of inflammatory parameters (edema, inflammatory infiltrate, leukocyte margination and hyperemia), particularly in the 4 th week after LPS application. Cytokines and leptin presented higher plasma levels in animals with dyslipidemia, but SLPI, considered a molecule involved in tissue protection, mostly in situations of inflammation or infection, was reduced in these animals compared to their controls. Our results allow us to conclude that dyslipidemia may alter the inflammatory response to infection.

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Ex vivo investigation of blood monocyte and platelet behaviour in pigs maintained on an atherogenic diet

Cited by 5 publications

References 18 publications

Macrophages in Immunopathology of Atherosclerosis: A Target for Diagnostics and Therapy

Macrophages in Immunopathology of Atherosclerosis: A Target for Diagnostics and Therapy

Monocytes as a diagnostic marker of cardiovascular diseases

Efeito da dislipidemia sobre a capacidade de defesa - estudo experimental

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