Ex vivo laser thermoplasty of whole costal cartilages

Sviridov, Аlexander P.; Захаркина, О. Л.; Ignatieva, Natalia; Vorobieva, N. N.; Bagratashvili, Nodar V.; Plyakin, V. A.; Kulik, I. O.; Sarukhanyan, O. O.; Минаев, В. П.; Лунин, В. В.; Баграташвили, В. Н.

doi:10.1002/lsm.22233

Cited by 7 publications

(4 citation statements)

References 21 publications

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“…Thus the most fragile and potentially thermal‐damage‐sensitive surface the cornea is inherently protected from overheating. For cartilage samples, the fact of sub‐surface location of the temperature maximum was theoretically predicted and experimentally demonstrated using an IR camera that visualized axial temperature distribution over one side of a rectangular sample heated from the orthogonal side . For cornea, this fact is demonstrated here for the first time by visualizing the temperature‐induced strain directly in the tissue bulk.…”

Section: Results and Their Interpretationmentioning

confidence: 80%

Optical coherence elastography for strain dynamics measurements in laser correction of cornea shape

Zaitsev

Matveyev

Matveev

et al. 2017

Journal of Biophotonics

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We describe the use of elastographic processing in phase-sensitive optical coherence tomography (OCT) for visualizing dynamics of strain and tissue-shape changes during laser-induced photothermal corneal reshaping, for applications in the emerging field of non-destructive and non-ablative (non-LASIK) laser vision correction. The proposed phase-processing approach based on fairly sparse data acquisition enabled rapid data processing and near-real-time visualization of dynamic strains. The approach avoids conventional phase unwrapping, yet allows for mapping strains even for significantly supra-wavelength inter-frame displacements of scatterers accompanied by multiple phase-wrapping. These developments bode well for real-time feedback systems for controlling the dynamics of corneal deformation with 10-100 ms temporal resolution, and for suitably long-term monitoring of resultant reshaping of the cornea. In ex-vivo experiments with excised rabbit eyes, we demonstrate temporal plastification of cornea that allows shape changes relevant for vision-correction applications without affecting its transparency. We demonstrate OCT's ability to detect achieving of threshold temperatures required for tissue plastification and simultaneously characterize transient and cumulative strain distributions, surface displacements, and scattering tissue properties. Comparison with previously used methods for studying laser-induced reshaping of cartilaginous tissues and numerical simulations is performed.

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Section: Results and Their Interpretationmentioning

confidence: 80%

Optical coherence elastography for strain dynamics measurements in laser correction of cornea shape

Zaitsev

Matveyev

Matveev

et al. 2017

Journal of Biophotonics

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“…In the samples under study, the 1.68‐mm laser radiation is mainly absorbed by water (70%). The effective absorption coefficient amounts to 7 cm –1 and the characteristic absorption depth is around 1.5 mm, which corresponds to the size of the laser spot zone. The size of the specimens heated in the calorimeter furnace was almost the same.…”

Section: Discussionmentioning

confidence: 99%

“… On the other hand, the thermal modification of the connective tissue matrix can lead to targeted alterations in its physicochemical properties, such as mechanical properties, permeability, tissue shrinkage and cartilage reshaping. Clinically, laser energy has been used to thermally modify connective tissue. The applying of moderate‐intensity laser radiation to heat tissue has several advantages over other heat sources: the localization and size of the heating zone and temperature dynamics are well controlled due to variations in wavelength and laser settings.…”

Section: Introductionmentioning

confidence: 99%

Transformation of the dermal collagen framework under laser heating

Ignatieva

Захаркина

Dadasheva

et al. 2019

Journal of Biophotonics

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The aim of this study was to compare between the changes undergone by the dermal collagen framework when heated by IR laser radiation and by traditional means and to reveal the specific features of the dermal matrix modification under moderate IR laser irradiation. Rabbit skin specimens were heated to 50°C, 55°C, 60°C and 65°C in a calorimeter furnace and with a 1.68‐μm fiber Raman laser. The proportion of the degraded collagen macromolecules was determined by differential scanning calorimetry. Changes in the architectonics of the collagen framework were revealed by using standard, phase‐contrast, polarization optical and scanning electron microscopy techniques. The collagen denaturation and dermal matrix amorphization temperature in the case of laser heating proved to be lower by 10°C than that for heating in the calorimeter furnace. The IR laser treatment of the skin was found to cause a specific low‐temperature (45°C‐50°C) transformation of its collagen framework, with some collagen macromolecules remaining intact. The transformation reduces to the splitting of collagen bundles and distortion of the course of collagen fibers. The denaturation of collagen macromolecules in the case of traditional heating takes its course in a threshold manner, so that their pre‐denaturation morphological changes are insignificant.

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“…The technique based on laser reshaping is a painless, bloodless procedure which substantially decrease the time of surgery and allows to avoid the long‐term post‐clinical rehabilitation. As the cartilage is the ideal natural material for implantation, the methods of laser‐assisted fabrication of cartilage implants of controllable shape are being developed and studied . The main goals are the effective relaxation of internal mechanical stress and stability of the new shape with the minimal affection on cartilage cells and matrix.…”

Section: Introductionmentioning

confidence: 99%

Effect of anisotropy and drying of costal cartilage on its optical transmittance in laser reshaping of implants with 1, 2, and 3 mm in thickness

et al. 2016

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The effect of structural anisotropy of costal cartilage reveals itself in the increasing scattering of IR radiation with λ = 1,560 nm passing crosswise the collagen orientation when tissue water content is decreased. The radiation with λ = 1,560 nm is effective to perform the reshaping for cartilage of 1-3 mm in thickness; however, for 3 mm, the residual mechanical stress should be taken into account. Lasers Surg. Med. 48:887-892, 2016. © 2016 Wiley Periodicals, Inc.

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Ex vivo laser thermoplasty of whole costal cartilages

Cited by 7 publications

References 21 publications

Optical coherence elastography for strain dynamics measurements in laser correction of cornea shape

Optical coherence elastography for strain dynamics measurements in laser correction of cornea shape

Transformation of the dermal collagen framework under laser heating

Effect of anisotropy and drying of costal cartilage on its optical transmittance in laser reshaping of implants with 1, 2, and 3 mm in thickness

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