1987
DOI: 10.1515/znb-1987-0410
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Examination of a New Cross-Linker in Monoclonal Antibody Reaction Against the C-Terminus of Tobacco Mosaic Virus Coat Protein

Abstract: The C-terminal tetrapeptide Gly-Pro-Ala-Thr of TMV coat protein was linked to poly- (L-lysine) of 37,300 and 80,000 daltons and to human serum albumin using different ratios of tetrapeptide to carrier. The new hydrazidosuccinyl HSc cross-linker effected the specific amino terminal attachment of the tetrapeptide by azide coupling. The synthetic peptide and its conjugates were controlled by various methods including 13C NMR and CD. Using a conjugate containing 74 tetrapeptide residue… Show more

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Cited by 5 publications
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