Examination of a role for idiotypy in the disease remission of a long-term survivor of adult T cell leukemia treated with anti-Tac antibody

Kingsbury, Gillian A.; Waldmann, Thomas A.; Junghans, Richard P.

doi:10.1038/sj.leu.2401048

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…Our patients generally had a poor response to chemotherapy. Treatment, therefore, remains disappointing, although there is interest in newer therapies, such as monocolonal antibodies and cytotoxic cells directed against viral antigens; these include anti‐Tac and anti‐CD25 antibodies 11,12 . Other therapies under investigation include arsenic trioxide (As2O3) plus α‐interferon, which is thought to have a synergistic effect to inhibit malignant cell growth or induce apoptosis 13 .…”

Section: Discussionmentioning

confidence: 99%

Adult T‐cell leukemia/lymphoma in Taiwan: An analysis of 17 patients and review of the literature

Lee

Chang

et al. 2010

Asia-Pac J Clncl Oncology

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Section: Discussionmentioning

confidence: 99%

Adult T‐cell leukemia/lymphoma in Taiwan: An analysis of 17 patients and review of the literature

Lee

Chang

et al. 2010

Asia-Pac J Clncl Oncology

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“…Myelopoietin is the first chimeric protein to be presented and mutagenized on phage, except perhaps, single-chain Fv antibody fragments (ScFvs, consisting of the variable heavy or V H and variable light or V L domains of antibodies linked by a flexible peptide, [34][35][36][37] ). ScFvs are chimeric proteins, though we view them as a special case.…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Phage display mutagenesis of the chimeric dual cytokine receptor agonist myelopoietin

Ibdah²,

Valkenburgh

et al. 2001

Leukemia

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Myelopoietins comprise a class of chimeric cytokine receptor agonists consisting of an hIL-3 (human interleukin-3) receptor agonist and an hG-CSF (human granulocyte colony-stimulating factor) receptor agonist linked head-to-tail at their respective carboxy and amino termini. The combination of an early acting cytokine (hIL-3) with a late acting one (hG-CSF) allows efficient hematopoeitic reconstruction following myeloablative insult, and drives differentiation of non-myelocytic lineages (ie thrombocytic lineages) that are inaccessible using hG-CSF alone, in both preclinical models and clinical settings. A myelopoietin species was displayed and mutagenized on filamentous bacteriophage: both component agonists of myelopoietin were presented in biologically functional conformations as each recognized its corresponding receptor. Five amino acid positions in a short region of the hG-CSF receptor agonist module of myelopoietin that had been identified as important for proliferative activity were mutagenized. Display was used because it allows very 'deep' mutagenesis at selected residues: Ͼ10 5 substitution variants were affinity-screened using the hG-CSF receptor and 130 new, active variants of myelopoietin were identified and characterized. None of the selected variants were significantly more active than the parental myelopoietin species in a hG-CSF-dependent cell proliferation assay, though many were as active. Many of these relatively high-activity variants contained parental amino acids at several positions, suggesting the parental sequence may already be optimal at these positions for the assays used, and potentially accounting for the failure to identify enhanced bioactivity variants. Analysis of substitutions of high-activity variants complements and extends previous alanine scanning, and other genetic and biochemical data for hG-CSF variants. Leukemia (2001) 15, 1277-1285.

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“…The anti-idiotype (anti-Id) phage Fab against mouse anti-Tac antibody (MAT) was cloned by combinatorial phage display techniques from B cells of a patient who was treated with MAT (6). An anti-tetanus toxoid (anti-TT) phage Fab was used as a nonspecific control (gift of D. Burton, Scripps Research Institute, La Jolla, CA).…”

Section: Phage Fab Clonesmentioning

confidence: 99%

Direct Immunoprecipitation of Antigen with Phage Displaying Immunoglobulin Fragment

2000

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Phage libraries may display hormones, receptors, antibody fragments, etc., by fusion to phage envelope proteins. This report describes the direct precipitation of phage-Fab-antigen complexes by polyethylene glycol precipitation, resulting in highly selective and efficient recovery of antigen from complex mixtures without nonspecific protein contamination. The method demonstrates efficiency and specific recovery of phage-Fab-antigen complexes from a background of a complex mixture of unrelated proteins as may occur in the analysis of biological specimens. This simple, fast, and effective method allows isolation and characterization of target antigens, with no need to further process Fab or sFv, and may reasonably be extended to isolate any interacting partner molecule for any displayed protein.

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Examination of a role for idiotypy in the disease remission of a long-term survivor of adult T cell leukemia treated with anti-Tac antibody

Cited by 4 publications

References 32 publications

Adult T‐cell leukemia/lymphoma in Taiwan: An analysis of 17 patients and review of the literature

Adult T‐cell leukemia/lymphoma in Taiwan: An analysis of 17 patients and review of the literature

Phage display mutagenesis of the chimeric dual cytokine receptor agonist myelopoietin

Direct Immunoprecipitation of Antigen with Phage Displaying Immunoglobulin Fragment

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