Examination of conditions for regular internal quality control in identification of microorganisms using MALDI-TOF MS

Sogawa, Kazuyuki; Kiyosuke, Makiko; Hattori, Kéiko; Murata, Syota; Watari, Tomohisa; Kusaba, Koji; Saeki, Yûji; Horiuchi, Kazuki; Yasuda, Kazunari; Uechi, Kohei; Okumura, Hajime; Sekiguchi, Satoshi; Fujinaga, Azumi; Murakami, Masami; Nakayama, Tomohiro

doi:10.4265/bio.26.99

Cited by 3 publications

(7 citation statements)

References 11 publications

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“…The detection limit of the MALDI-TOF MS technique is at least 10 2 –10 4 cells, depending on the bacterial species, even if a small amount of microbial mass is enough to obtain an identification with a good score [ 21 , 22 , 23 , 26 ].…”

Section: Maldi-tof Ms: Principles Of the Proceduresmentioning

confidence: 99%

“…The computer software associated with the machine MALDI Biotyper (Bruker Daltonics GmbH, Bremen, Germany) uses this “score”; a score value of 2.0 is considered a reliable criterion of bacterial species identification, although the spectra obtained from a single strain are not identical in repeated runs because of a method-inherent noise. Score values between 1.7 and 2.0 are considered reliable for genus identification [ 22 , 23 , 24 , 25 , 27 , 28 , 29 , 30 , 31 , 32 ]. The machine MALDI VITEK MS (bio-Mérieux, Mercy L’Étoile, France) produces a confidence value, which can be reported as a percentage up to 99.9%.…”

Section: Maldi-tof Ms: Principles Of the Proceduresmentioning

confidence: 99%

“…If no identification is provided, the isolate is considered unidentified. The Biotyper machine reports the identification of each organism with a numerical “score,” and, according to the manufacturer, scores of ≥2.0 and ≥1.7 represent acceptable probable species- and genus-level identifications, respectively [ 22 , 23 , 24 , 27 , 28 ].…”

Section: Maldi-tof Ms: Principles Of the Proceduresmentioning

confidence: 99%

“…Appropriate pretreatments of the sample before the analysis can make the identification of microorganisms more efficient, rapid, and accurate when used in association with the implementation of the MALDI-TOF MS database with protein spectra of different bacterial strains. There have been numerous studies reporting protocols for the pretreatment of the sample to improve the identification of microorganisms by MALDI-TOF MS, but the effect of the pretreatment on microbial identification is often neglected [ 23 ].…”

Section: Pretreatment Of the Samplesmentioning

confidence: 99%

“… Milestones of the history of the MALDI-TOF MS application in clinical microbiology [ 1 , 2 , 18 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. …”

Section: Figurementioning

confidence: 99%

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MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology Laboratory

Calderaro,

Chezzi

2024

Microorganisms

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Matrix-Assisted Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) in the last decade has revealed itself as a valid support in the workflow in the clinical microbiology laboratory for the identification of bacteria and fungi, demonstrating high reliability and effectiveness in this application. Its use has reduced, by 24 h, the time to obtain a microbiological diagnosis compared to conventional biochemical automatic systems. MALDI-TOF MS application to the detection of pathogens directly in clinical samples was proposed but requires a deeper investigation, whereas its application to positive blood cultures for the identification of microorganisms and the detection of antimicrobial resistance are now the most useful applications. Thanks to its rapidity, accuracy, and low price in reagents and consumables, MALDI-TOF MS has also been applied to different fields of clinical microbiology, such as the detection of antibiotic susceptibility/resistance biomarkers, the identification of aminoacidic sequences and the chemical structure of protein terminal groups, and as an emerging method in microbial typing. Some of these applications are waiting for an extensive evaluation before confirming a transfer to the routine. MALDI-TOF MS has not yet been used for the routine identification of parasites; nevertheless, studies have been reported in the last few years on its use in the identification of intestinal protozoa, Plasmodium falciparum, or ectoparasites. Innovative applications of MALDI-TOF MS to viruses’ identification were also reported, seeking further studies before adapting this tool to the virus’s diagnostic. This mini-review is focused on the MALDI-TOF MS application in the real life of the diagnostic microbiology laboratory.

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Section: Maldi-tof Ms: Principles Of the Proceduresmentioning

confidence: 99%

Section: Maldi-tof Ms: Principles Of the Proceduresmentioning

confidence: 99%

Section: Maldi-tof Ms: Principles Of the Proceduresmentioning

confidence: 99%

Section: Pretreatment Of the Samplesmentioning

confidence: 99%

“… Milestones of the history of the MALDI-TOF MS application in clinical microbiology [ 1 , 2 , 18 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. …”

Section: Figurementioning

confidence: 99%

See 3 more Smart Citations

MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology Laboratory

Calderaro,

Chezzi

2024

Microorganisms

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Examination of Conditions for External Quality Control in Identification of Microorganisms using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

et al. 2022

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was approved for medical use in 2011 and is currently used as a rapid, accurate and lowcost technique for bacterial identification. External quality control for medical analysis is monitored using tests of the Japanese Association of Medical Technologists and Prefectural Association of Clinical Laboratory Technologists and through user surveys of reagent and equipment manufacturers. However, external quality control of bacterial typing using MS is not performed. Therefore, we examined procedures for evaluating quality control of bacterial typing using an identification reliability index at 38 facilities.

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External quality control survey on identification of microorganisms using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

SOGAWA,

FUJINAGA,

OKUMURA

et al. 2024

Journal of Microorganism Control

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Examination of conditions for regular internal quality control in identification of microorganisms using MALDI-TOF MS

Cited by 3 publications

References 11 publications

MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology Laboratory

MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology Laboratory

Examination of Conditions for External Quality Control in Identification of Microorganisms using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

External quality control survey on identification of microorganisms using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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