Examination of Ovine Foetal Fluid for Antibodies to Toxoplasma Gondii by the Dye Test and an Indirect Immunofluorescence Test Specific for Igm

Hunter, Damien S.; Chadwick, Paul; Balfour, A H; Bridges, J. B.

doi:10.1016/s0007-1935(17)31186-7

Cited by 24 publications

(3 citation statements)

References 5 publications

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“…SL19 also had a positive IHAT titre. The changes in titre may be of importance for SL 8,19, but the IgG blots were identical. The bands were surprisingly well stained in view of the low antibody titres of the sera.…”

Section: Resultsmentioning

confidence: 89%

Toxoplasma infection and systemic lupus erythematosus: analysis of the serological response by immunoblotting.

Noel

Balfour²,

Wilcox³

1993

Journal of Clinical Pathology

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Section: Resultsmentioning

confidence: 89%

Toxoplasma infection and systemic lupus erythematosus: analysis of the serological response by immunoblotting.

Noel

Balfour²,

Wilcox³

1993

Journal of Clinical Pathology

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“…In order to maximise the detection of toxoplasma-mismatched patients, all samples from donors with a titre of 16 or more and from recipients with a titre of 32 or more were provisionally regarded as positive for Z gondii antibody. If a transplant patient was, by these criteria, mismatched for Zgondii, then serum samples from both organ donor and recipient were sent to the Toxoplasma Reference Laboratory, where dye test [4], indirect haemagglutination [3] and Zgondii IgM enzyme-linked immunosorbent assay [6] tests were performed. In the meantime, patients were started on a course of pyrimethamine prophylaxis, which was discontinued if they were found by the Reference Laboratory not to be mismatched for Zgondii.…”

Section: Toxoplasma Gondii Serological Methodsmentioning

confidence: 99%

Efficacy of pyrimethamine for the prevention of donor-acquired Toxoplasma gondii infection in heart and heart-lung transplant patients

et al. 1992

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Seven (11%) of the first 65 patients who received heart transplants at Papworth Hospital were mismatched for Toxoplasma gondii. Of these, four (57%) experienced T. gondii infection and two died. The remaining two had severe symptoms and received anti-T-gondii chemotherapy for a year after transplantation. In an attempt to reduce the impact of donor-acquired T. gondii in our heart transplant recipients, we decided in April 1984 to give prophylactic pyrimethamine to all T. gondii-mismatched patients. In this study, 7 years later, we review the efficacy of this policy. Five of 37 (14%) patients given prophylactic pyrimethamine acquired T. gondii infection; only one was symptomatic, and none died. This compares with 100% symptomatic infection in the pre-1984 patients, who did not receive prophylactic pyrimethamine. We believe that our experience has shown that pyrimethamine is effective in reducing the incidence and severity of primary donor-acquired T. gondii infection in mismatched heart and heart-lung transplant recipients.

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“…2,12 Routine diagnosis and epidemiologic studies of veterinary and medical diseases require the use of diagnostic tests or kits that have been validated for the target population. 9 Several diagnostic tests and kits have been developed for ovine toxoplasmosis, including the direct agglutination test, 18 dye test, 8,15 enzyme-linked immunosorbent assay (ELISA), 18,19,22 indirect hemagglutination test, 4 indirect immunoflourescent antibody test, 19 latex agglutination test, 25 modified agglutination test (MAT), 4 polymerase chain reaction, 16,27 and Western blotting. 6 A diagnosis of ovine toxoplasmosis can also be made through identification of pathologic changes and isolation of the parasite in mice.…”

mentioning

confidence: 99%

Evaluation of Kinetics and Single-Read Enzyme-Linked Immunoassays for Detection ofToxoplasma GondiiAntibodies in Sheep

et al. 2002

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Abstract.A kinetics enzyme-linked immunosorbent assay (ELISA) and a single-read ELISA for the detection of ovine anti-Toxoplasma gondii IgG were developed and optimized. During the kinetics assay, 3 optical densities were obtained for each serum sample at intervals of 45 seconds, and the results were presented as average slopes (replicates of 2) of the reaction rate between bound enzyme and substrate solution. The kinetics ELISA was stopped 5 minutes after dispensing the substrate to constitute the single-read ELISA, and the results were presented as average optical densities for duplicates of each sample. Performance of the assays was evaluated using the modified agglutination test (MAT) as the ''gold standard.'' There was a high level of agreement between both ELISAs and the MAT, as measured by Pearson correlation coefficients, kappa statistics, and the area under the receiver operating characteristics curves. The single-read ELISA was as accurate as the kinetics ELISA, with a sensitivity of 89% and a specificity of 96%.

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Examination of Ovine Foetal Fluid for Antibodies to Toxoplasma Gondii by the Dye Test and an Indirect Immunofluorescence Test Specific for Igm

Cited by 24 publications

References 5 publications

Toxoplasma infection and systemic lupus erythematosus: analysis of the serological response by immunoblotting.

Toxoplasma infection and systemic lupus erythematosus: analysis of the serological response by immunoblotting.

Efficacy of pyrimethamine for the prevention of donor-acquired Toxoplasma gondii infection in heart and heart-lung transplant patients

Evaluation of Kinetics and Single-Read Enzyme-Linked Immunoassays for Detection ofToxoplasma GondiiAntibodies in Sheep

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