Examination of sensorimotor performance following middle cerebral artery occlusion in rats

Reglődi, Dóra; Tamás, Andrea; Lengvári, Istvàn

doi:10.1016/s0361-9230(02)00962-0

Cited by 110 publications

(58 citation statements)

References 51 publications

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“…However, G-CSF treatment increased the rate at which MCAO animals gained weight. Other studies have reported similar weight losses after MCAO (Reglodi et al, 2003) or sham surgery (Barone et al, 1992). Middle cerebral artery occlusion, 60 mins, in Figure 4 Granulocyte colony-stimulating factor treatment improves cognitive performance.…”

Section: Discussionmentioning

confidence: 64%

“…In these studies, 50 or 60 mg/kg G-CSF was administered via a single subcutaneous injection, and their findings formed the basis for the present study. However, changes in lesion volume do not always represent changes in functional outcome and, while some studies show that lesion size correlates with functional deficit (Rogers et al, 1997), others show it does not (Hattori et al, 2000;Reglodi et al, 2003;Wahl et al, 1992). In the current study, we measured lesion volume at 48 hours, and so it is not possible to rule out the possibility that G-CSF simply delayed the development of the lesion rather than reduced it.…”

Section: Discussionmentioning

confidence: 99%

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G-CSF Reduces Infarct Volume and Improves Functional Outcome after Transient Focal Cerebral Ischemia in Mice

Gibson

Bath

Murphy

2005

J Cereb Blood Flow Metab

156

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Growth factors possess neuroprotective and neurotrophic properties in vitro, but few have been extensively studied in vivo after stroke. In the present study, we investigated the potential functional benefits of granulocyte colony-stimulating factor (G-CSF) administration after focal cerebral ischemia. Male mice underwent 60-minute middle cerebral artery occlusion (MCAO) and received G-CSF (50 lg/kg, subcutaneously) or vehicle (saline) at the onset of reperfusion. Granulocyte colony-stimulating factor-treated mice killed at 48 hours after MCAO revealed a 445% reduction (Po0.05) in lesion volume. In terms of body weight recovery, and in tests of motor (grid test and rotarod) and cognitive ability (water maze), MCAO significantly worsened the outcome in vehicletreated mice as compared with shams (Po0.05). However, G-CSF treatment was beneficial as, compared with vehicle, this significantly improved weight recovery and motor ability. This effect was most apparent on the water maze where G-CSF-treated mice were indistinguishable from shams in terms of acquiring the task. These results indicate long-term beneficial effects of a single dose of G-CSF administered on reperfusion, and illustrate the need to further investigate the mechanisms of G-CSF action.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

G-CSF Reduces Infarct Volume and Improves Functional Outcome after Transient Focal Cerebral Ischemia in Mice

Gibson

Bath

Murphy

2005

J Cereb Blood Flow Metab

156

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“…However, assessment of stroke lesion has been reported to be of limited value in murine MCAO models (Reglodi et al, 2002;Hattori et al, 2000). In murine MCAO models, specific functional and cognitive tests have been shown to better correlate with injury severity up to 21 days (Gibson and Murphy, 2004).…”

Section: Discussionmentioning

confidence: 99%

Effects of Combined Oral Conjugated Estrogens and Medroxyprogesterone Acetate on Brain Infarction Size after Experimental Stroke in Rat

Littleton-Kearney

Klaus

Hurn

2005

J Cereb Blood Flow Metab

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The reason that estrogen is strongly protective in various estrogen-deficient animal models while seemingly detrimental in postmenopausal women remains unclear. It hypothesized that prolonged oral medroxyprogesterone (MPA) plus oral conjugated equine estrogens (CEE) diminishes estrogen ability to reduce stroke damage in the rodent stroke model. To test the hypothesis, we fed ovariectomized rats CEE or MPA, or a combination of CEE and MPA (CEP), before inducing 120 min of reversible focal stroke, using the intraluminal filament model. After 22 h reperfusion, the brains were harvested and infarction volumes were quantified. Treatment with CEE alone or with CEP reduced cortical infarction volume. However, CEP failed to provide ischemic protection in subcortical regions. It was concluded that CEE alone, or with CEP, is neuroprotective in the cortex, but interactive effects between the hormones may counteract CEE beneficial effects in subcortical brain regions.

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“…Furthermore, after ischemic lesion, intracortically transplanted NSCs have been shown to migrate away from the initial graft location and to align themselves with the corpus callosum [44]. These and other studies suggest that NSCs are able to migrate to parts of the brain remote from the original The cylinder rearing test revealed no significant differences in the use of the contralesional (left) forepaw over time in either of the treatment groups, but indicated a trend towards improvement in the saline treated control group, which may be consistent with spontaneous functional recovery [45,46]. The lack of improvement in forepaw preference after NSC or NSC-OEC transplantation is not surprising, considering that any functional benefits as a result of the treatment would be marginal, and thereby difficult to evaluate at 3wpx.…”

Section: Discussionmentioning

confidence: 61%

Effects of Neural Stem Cell and Olfactory Ensheathing Cell Co-transplants on Tissue Remodelling After Transient Focal Cerebral Ischemia in the Adult Rat

et al. 2017

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Effects of neural stem cell and olfactory ensheathing cell cotransplants on tissue remodelling after transient focal cerebral ischemia in the adult rat. Neurochemical Research, 42(6), pp. 1599-1609.There may be differences between this version and the published version. You are advised to consult the publisher's version if you wish to cite from it.http://eprints.gla.ac.uk/136264/ with the host-tissue post-transplantation is poor. In this study, we address this challenge by testing whether co-grafting of NSCs with olfactory ensheathing cells (OECs), a special type of glia with proven neuroprotective, immunomodulatory, and angiogenic effects, can promote graft survival and host tissue remodelling. Transient focal cerebral ischemia was induced in adult rats by a sixty-minute middle cerebral artery occlusion (MCAo) followed by reperfusion. Ischemic lesions were verified by neurological testing and magnetic resonance imaging (MRI). Transplantation into the globus pallidus of NSCs alone or in combination with OECs was performed at two weeks post-MCAo, followed by histological analyses at three weeks post-transplantation. We found evidence of extensive vascular remodelling in the ischemic core as well as evidence of NSC motility away from the graft and into the infarct border in severely lesioned animals co-grafted with OECs. These findings support a possible role of OECs as part of an in situ tissue engineering paradigm for transplant mediated repair of ischemic brain lesions.

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Examination of sensorimotor performance following middle cerebral artery occlusion in rats

Cited by 110 publications

References 51 publications

G-CSF Reduces Infarct Volume and Improves Functional Outcome after Transient Focal Cerebral Ischemia in Mice

G-CSF Reduces Infarct Volume and Improves Functional Outcome after Transient Focal Cerebral Ischemia in Mice

Effects of Combined Oral Conjugated Estrogens and Medroxyprogesterone Acetate on Brain Infarction Size after Experimental Stroke in Rat

Effects of Neural Stem Cell and Olfactory Ensheathing Cell Co-transplants on Tissue Remodelling After Transient Focal Cerebral Ischemia in the Adult Rat

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