, "Examiner's finger-mounted nearinfrared spectroscopy is feasible to analyze cerebral and skeletal muscle oxygenation in conscious Chihuahuas," J. Biomed. Opt. 22(2), 026006 (2017), doi: 10.1117/1.JBO.22.2.026006. Abstract. To measure regional saturation of oxygen (rSO 2 ) of hemoglobin and total hemoglobin index (HbI) in the brain (through the molera of the head) and skeletal muscle (musculus gracilis) of conscious Chihuahua dogs using an examiner's finger-mounted near-infrared spectroscopy (NIRS) device, Toccare, we investigated brain and skeletal muscle NIRS in 48 Chihuahuas without severe disease. To measure rSO 2 and total HbI, a Toccare probe was placed on the molera of the head and musculus gracilis of each dog for real-time recording. Stable NIRS values were obtained within 10 s. We also examined the effect of anesthesia on rSO 2 and total HbI of a Chihuahua. Cerebral rSO 2 values (59% AE 7%) were significantly lower than those obtained at femoral regions (67% AE 6%), whereas total HbI values in the brain (0.38 AE 0.09) were significantly higher than those of the musculus gracilis (0.20 AE 0.05). Sedation with a combination of medetomidine and ketamine decreased cerebral rSO 2 along with a corresponding reduction in heart rate. Sevoflurane anesthesia with 100% O 2 maintained rSO 2 in the brain with an even lower heart rate. In conclusions, we measured brain and skeletal muscle rSO 2 of hemoglobin in conscious Chihuahuas using a newly developed NIRS device, Toccare, and found that changes in cerebral oxygenation levels were associated with administration of anesthetics. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.