Examining the Potential of Plasma-Assisted Pretreated Wheat Straw for Enzyme Production by Trichoderma reesei

Rodriguez-Gomez, Divanery; Lehmann, Linda Olkjær; Schultz-Jensen, Nadja; Bjerre, Anne Belinda; Hobley, Timothy John

doi:10.1007/s12010-012-9631-x

Cited by 29 publications

(7 citation statements)

References 33 publications

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“…Despite its relevance for application, there is less data available on suitability of “real” substrates for enzyme production. Studies have investigated the use of sugar cane bagasse ( n = 3) [88, 103, 107], herbaceous straw (wheat and rice straw, switch grass, corn stover, n = 10) [13, 15, 18, 76, 87, 99, 105, 108–110], and woody biomass ( n = 4) [15, 90, 93, 105]. Dependent on pretreatment conditions, agricultural residues contain up to 25% hemicellulose, mainly xylan with few substituents.…”

Section: Meta-analysis Of Enzyme Production By T Reesei Cultivated Omentioning

confidence: 99%

The influence of feedstock characteristics on enzyme production in Trichoderma reesei: a review on productivity, gene regulation and secretion profiles

Novy

Nielsen

Schink

et al. 2019

Biotechnol Biofuels

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Biorefineries, designed for the production of lignocellulose-based chemicals and fuels, are receiving increasing attention from the public, governments, and industries. A major obstacle for biorefineries to advance to commercial scale is the high cost of the enzymes required to derive the fermentable sugars from the feedstock used. As summarized in this review, techno-economic studies suggest co-localization and integration of enzyme manufacturing with the cellulosic biorefinery as the most promising alternative to alleviate this problem. Thus, cultivation of Trichoderma reesei, the principal producer of lignocellulolytic enzymes, on the lignocellulosic biomass processed on-site can reduce the cost of enzyme manufacturing. Further, due to a complex gene regulation machinery, the fungus can adjust the gene expression of the lignocellulolytic enzymes towards the characteristics of the feedstock, increasing the hydrolytic efficiency of the produced enzyme cocktail. Despite extensive research over decades, the underlying regulatory mechanisms are not fully elucidated. One aspect that has received relatively little attention in literature is the influence the characteristics of a lignocellulosic substrate, i.e., its chemical and physical composition, has on the produced enzyme mixture. Considering that the fungus is dependent on efficient enzymatic degradation of the lignocellulose for continuous supply of carbon and energy, a relationship between feedstock characteristics and secretome composition can be expected. The aim of this review was to systematically collect, appraise, and aggregate data and integrate results from studies analyzing enzyme production by T. reesei on insoluble cellulosic model substrates and lignocellulosic biomass. The results show that there is a direct effect of the substrate’s complexity (rated by structure, composition of the lignin–carbohydrate complex, and recalcitrance in enzymatic saccharification) on enzyme titers and the composition of specific activities in the secretome. It further shows that process-related factors, such as substrate loading and cultivation set-up, are direct targets for increasing enzyme yields. The literature on transcriptome and secretome composition further supports the proposed influence of substrate-related factors on the expression of lignocellulolytic enzymes. This review provides insights into the interrelation between the characteristics of the substrate and the enzyme production by T. reesei, which may help to advance integrated enzyme manufacturing of substrate-specific enzymes cocktails at scale.

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Section: Meta-analysis Of Enzyme Production By T Reesei Cultivated Omentioning

confidence: 99%

The influence of feedstock characteristics on enzyme production in Trichoderma reesei: a review on productivity, gene regulation and secretion profiles

Novy

Nielsen

Schink

et al. 2019

Biotechnol Biofuels

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“…The ethanol yield was relatively low and the process economics has the potential to be improved via the recovery and use of lignin byproducts and hemicellulose, as well as developing schemes to reduce ozone consumption for similar yields. Aside high delignification and carbohydrate preservation, pretreated substrates have shown potential for use in the production of enzymes by fungi such as Trichoderma reesei, with lower titres of cellulases and higher amount of xylanases recorded from autoclave sterilization of pretreated materials, compared to nonsterilized substrates with antibiotics added [215].…”

Section: Ozonolysismentioning

confidence: 99%

Chemical Pretreatment Methods for the Production of Cellulosic Ethanol: Technologies and Innovations

Bensah

Mensah

2013

International Journal of Chemical Engineering

277

105

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Pretreatment of lignocellulose has received considerable research globally due to its influence on the technical, economic and environmental sustainability of cellulosic ethanol production. Some of the most promising pretreatment methods require the application of chemicals such as acids, alkali, salts, oxidants, and solvents. Thus, advances in research have enabled the development and integration of chemical-based pretreatment into proprietary ethanol production technologies in several pilot and demonstration plants globally, with potential to scale-up to commercial levels. This paper reviews known and emerging chemical pretreatment methods, highlighting recent findings and process innovations developed to offset inherent challenges via a range of interventions, notably, the combination of chemical pretreatment with other methods to improve carbohydrate preservation, reduce formation of degradation products, achieve high sugar yields at mild reaction conditions, reduce solvent loads and enzyme dose, reduce waste generation, and improve recovery of biomass components in pure forms. The use of chemicals such as ionic liquids, NMMO, and sulphite are promising once challenges in solvent recovery are overcome. For developing countries, alkalibased methods are relatively easy to deploy in decentralized, low-tech systems owing to advantages such as the requirement of simple reactors and the ease of operation.

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“…Biomass type and carbonization conditions are important parameters that affect the pore size and chemical properties of biochar. The world annual production of wheat straw is about 529 million tons/year [18], and it contains high values of cellulose (34-40%), hemicellulose (20-25%) and lignin (20%) [19]. The low cost and abundance of organic functional groups in the wheat straw make it a potential adsorbent for the binding of heavy metals after carbonization.…”

Section: Introductionmentioning

confidence: 99%

Remediation of Emerging Heavy Metals from Water Using Natural Adsorbent: Adsorption Performance and Mechanistic Insights

et al. 2021

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The presence of potentially toxic metals in water causes a strong impact on environment and human health. In this study, activated biochar was produced by using chemical oxidation method from wheat straw as natural adsorbent and was employed for heavy metals competitive remediation. The morphology, structure, and chemical properties of biochar before and after adsorption were characterized by FTIR, XRD, SEM and EDX mapping techniques. The competitive adsorption efficiency of adsorbent for divalent cadmium (Cd) and lead (Pb) from contaminated water was investigated by using wide range of several initial metal concentration, contact time and pH. Maximum adsorption of Cd(II) and Pb(II) was found in the pH range of 6–8. The adsorption capacity for Cd(II) and Pb(II) was 8.85 and 9.03 mg/g, respectively. Thermodynamics parameters and kinetic models were applied to adsorption data. The isotherm data followed Langmuir model, corresponding to monolayer adsorption of the two ions in the contaminated water. The kinetic data followed the pseudo 2nd order kinetics model, which authenticates the chemisorption nature. The thermodynamic study indicated that Cd adsorption is a spontaneous exothermic process while Pb adsorption is an endothermic process. Mineral precipitation, surface complexation, and cation-π interactions are the major remediation strategies for Cd(II) and Pb(II).

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Examining the Potential of Plasma-Assisted Pretreated Wheat Straw for Enzyme Production by Trichoderma reesei

Cited by 29 publications

References 33 publications

The influence of feedstock characteristics on enzyme production in Trichoderma reesei: a review on productivity, gene regulation and secretion profiles

The influence of feedstock characteristics on enzyme production in Trichoderma reesei: a review on productivity, gene regulation and secretion profiles

Chemical Pretreatment Methods for the Production of Cellulosic Ethanol: Technologies and Innovations

Remediation of Emerging Heavy Metals from Water Using Natural Adsorbent: Adsorption Performance and Mechanistic Insights

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