Exchange of Lipids Between Spermatozoa and Seminal Plasma in Normal and Pathological Human Semen

Huacuja, L; Delgado, N. M.; Calzada, L; Wens, A.; Reyes, R.; Pedrón, Nieves; Rosado, Adolfo

doi:10.3109/01485018108999329

Cited by 30 publications

(17 citation statements)

References 23 publications

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“…The decreased phospholipid values in spermatozoa thus suggest that there is a change in the phospholipid component of the sperm membrane which plays a major role in the fertilizing ability of spermatozoa. An aberrant phospholipid level in spermatozoa has also been reported previously in oligoasthenospermic and asthenozoospermic infertile patients (Huacuja et al, 1981). Phospholipids along with cholesterol play a significant role in maintaining the cohesiveness of sperm membrane structure and their ability to withstand physical and physiological stress (see Philips, 1972).…”

Section: Discussionmentioning

confidence: 69%

Effect of chronic alcoholism on semen—studies on lipid profiles

et al. 1993

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The effect of chronic alcoholism on various seminal parameters (sperm concentration, rate of forward motility, percentage of abnormal spermatozoa, lipid profiles of seminal plasma and spermatozoa) was studied together with the serum levels of testosterone and oestradiol. In chronic alcoholics there was a marked reduction in sperm concentration and in the rate of their forward motility, and increase in the number of spermatozoa with morphological abnormalities when compared to age-matched normal fertile subjects. Serum levels of testosterone were decreased while oestradiol levels were increased in chronic alcoholic men. Studies of lipid profiles showed a marked decrease in the total phospholipid concentration in spermatozoa, primarily in sphingomyelin, phosphatidyl choline and ethanolamine fractions. The cholesterol:phospholipid ratio in spermatozoa was increased in alcoholics. In the seminal plasma of chronic alcoholics, there was a decrease in total lipid, in glyceride glycerol and in free and esterified cholesterol. Of the phospholipid classes, sphingomyelin and phosphatidyl ethanolamine showed a significant reduction. In general, the present study provides evidence for the adverse effects of chronic alcoholism on serum hormones, sperm count, morphology, motility and seminal lipid profiles. These may be responsible for the fertility disorders common in chronic alcoholics.

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Section: Discussionmentioning

confidence: 69%

Effect of chronic alcoholism on semen—studies on lipid profiles

et al. 1993

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“…We used Chol as a molecule that provides rigidness to the membrane and polyunsaturated acids as agents promoting membrane fluidity. Sebastian et al (1987) and Huacuja et al (1981) suggested that there is a positive correlation between seminal PL and fertility and a negative correlation between seminal neutral lipids (i.e., Chol) and fertility. However little work is available on the pattern of different classes of lipids, their fractions in the seminal plasma (SP), and their influence on mammalian spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

Cholesterol and polyunsaturated acid enriched diet: Effect on kinetics of the acrosome reaction in rabbit spermatozoa

Diaz‐Fontdevila

Bustos‐Obregón

1993

Molecular Reproduction Devel

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In this study we examined the effect of cholesterol (Diet 2), cholesterol and fish oil (FO) polyunsaturated acid (Diet 3), and polyunsaturated acid (Diet 4) enriched diets upon the acrosome reaction (AR) of New Zealand White rabbit spermatozoa. Male rabbits fed with cholesterol alone or with FO increased their cholesterol and LDL-cholesterol serum levels after 15 days of diet. Ten semen samples were obtained after 2 months of diet. Our results suggest that hypercholesterolemia and hypertriglyceridemia in male rabbits could produce a decreased capacity of sperm AR after 4 h (0%, 0%, and 60% lower than the control), 6 h (0%, 68%, and 44%), or 8 h (58%, 52% and 32%) of incubation in capacitating medium. Another set of experiments were made with lysophosphatidylcholine (LPC), 80 micrograms/ml, and the same pattern of AR was seen. Nevertheless, the high cholesterol and total lipids (TL) levels in serum did not affect the cholesterol levels in seminal plasma (SP) but affect the SP total lipids. The diminished capacity of rabbit sperm to undergo the AR was not reverted by in vitro incubation with the Shinitsky medium for cholesterol depletion (MDC). These results indirectly suggest that the cholesterol/phospholipid ratio in hypercholesterolemic sperm is similar to that of controls and are in agreement with preliminary studies made in our laboratory that evidenced the same cholesterol/phospholipid ratio in rabbit sperm from hypercholesterolemic animals than from controls.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…The lipid profile in the sperm tail membrane is associated with physical properties that facilitate sperm motility and velocity (Connor et al 1998). Antioxidant (Lenzi et al 2000, Strzeżek et al 2004 and cholesterol contents in the seminal fluids (Huacuja et al 1981, Morales et al 2008, Saez et al 2011, as well as cholesterol acceptors that facilitate cholesterol efflux from the sperm during capacitation (Saez et al 2011), positively affect sperm quality.…”

Section: Introductionmentioning

confidence: 99%

Season-induced variation in lipid composition is associated with semen quality in Holstein bulls

et al. 2013

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Season-induced variation in fatty acid and cholesterol composition in bovine semen has been associated with semen quality. Given the specific roles of the various semen compartments (seminal fluids, sperm head, and sperm tail) in fertilization, we hypothesized that environmental-stress-induced alterations in the lipid composition of a specific compartment might impair semen quality and sperm function. Semen samples were collected from five mature Holstein-Friesian bulls during the summer (August to September) and winter (December to January). Semen was evaluated by computerized sperm-quality analyzer, calibrated for bulls' semen, and centrifuged to separate the spermatozoa from the seminal fluids. The spermatozoal fraction was sonicated to separate the sperm head and tail compartments. Cold lipid extraction was performed with chloroform:methanol (2:1, vol/vol). Lipids were identified and quantified by gas chromatography. Seasonal variation was found in both physiological and structural parameters. The proportion of spermatozoa defined as morphologically normal was higher in the winter, with higher motility, progressive motility, and velocity relative to summer samples. Lipid composition within fractions varied between seasons with prominent impairment in the tail compartment, characterized by high saturated fatty acid, low polyunsaturated fatty acid, and low cholesterol concentrations during the summer. Given the association between alterations in lipid composition and reduced sperm motility and velocity during the summer, it is suggested that lipid composition might serve to predict sperm quality.

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Exchange of Lipids Between Spermatozoa and Seminal Plasma in Normal and Pathological Human Semen

Cited by 30 publications

References 23 publications

Effect of chronic alcoholism on semen—studies on lipid profiles

Effect of chronic alcoholism on semen—studies on lipid profiles

Cholesterol and polyunsaturated acid enriched diet: Effect on kinetics of the acrosome reaction in rabbit spermatozoa

Season-induced variation in lipid composition is associated with semen quality in Holstein bulls

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