Excretion of Lipoteichoic Acid by Group A Streptococci

Alkan, Michael; Beachey, Edwin H.

doi:10.1172/jci108979

Cited by 111 publications

(13 citation statements)

References 19 publications

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“…The main peak of radioactivity (fractions 20 to 30; referred to as peak 1) was observed in the position of LTA, as found by others (1,8), and failed to absorb ultraviolet light at 260 nm. This was followed by a smaller peak (fractions 35 to 45; referred to as peak 2), which has been previously reported to be deacylated LTA (1,8). We also observed that treatment of peak 1 material with methanolic KOH (7) quantitatively converted it to a form which eluted exclusively in the position of peak 2.…”

Section: Resultssupporting

confidence: 71%

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Oxacillin-Induced Lysis of Staphylococcus aureus

Raynor

Scott

Best

1979

Antimicrob Agents Chemother

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Six clinical isolates of Staphylococcus aureus were compared for their relative susceptibilities to the killing effects of oxacillin. Three of the strains had minimum bactericidal concentrations which were >10 times the minimum bacteriostatic concentration for this antibiotic and were designated tolerant (Tol+). The other strains had minimum bactericidal concentrations which were comparable to the minimum bacteriostatic concentration (Tol-)

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Section: Resultssupporting

confidence: 71%

“…Fractions (3 ml) were collected at a rate of 15 ml/h and analyzed for radioactivity, phosphate (6), and nucleic acids (absorption at 260 nm). This procedure has previously been employed by others to isolate and characterize LTA from other bacteria (1,8).…”

mentioning

confidence: 99%

Oxacillin-Induced Lysis of Staphylococcus aureus

Raynor

Scott

Best

1979

Antimicrob Agents Chemother

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“…These components appear to be effective not only on the intact bacterial surface but also in soluble form. Both the group-and typespecific antigens are released spontaneously by GBS during infection (3,8), while LTA may be abundantly released by the effects of ␤-lactam antibiotics (1). Although the exact role of these agents is still unclear, it is reasonable to hypothesize that they may act alone or synergistically to produce cytokine-mediated pathophysiologic changes in GBS disease.…”

Section: Discussionmentioning

confidence: 99%

Soluble antigens from group B streptococci induce cytokine production in human blood cultures

et al. 1997

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Group B streptococcal antigens stimulated tumor necrosis factor alpha (TNF-␣), interleukin-1 (IL-1), and IL-6 production in human blood cultures in a concentration-and time-dependent fashion. The minimal concentrations of type-specific polysaccharides, lipoteichoic acid, and group-specific polysaccharide required to produce these effects were, respectively, 0.01, 1, and 10 g/ml. Cell separation experiments indicated that monocytes were the cell type mainly responsible for cytokine production. Time course studies indicated that TNF-␣ was released before the other cytokines. TNF-␣, however, did not appear to directly induce IL-1␤, as shown by blockade experiments with anti-TNF-␣ antibodies. IL-6 levels were moderately but significantly decreased by anti-TNF-␣. These data indicate that several products from group B streptococci are able to directly stimulate human monocytes to release TNF-␣, IL-1␤, and IL-6. These findings may be clinically relevant, since proinflammatory cytokines can mediate pathophysiologic changes during sepsis.

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“…Concomitantly, the treated organisms lost their adhesive properties. 19 ) Streptomycin suppressed the formation and expression of the mannose-specific ligand in E. coli, probably by acting on the bacterial ribosome to induce misreading of mRNA, which often led to abnormal protein synthesis. 20 ) We tested the mannose-resistant hemagglutination inactiṽ ation activity of several well-known antibiotics.…”

Section: Biological Propertiesmentioning

confidence: 99%