Exercise-induced piezoelectric stimulation for cartilage regeneration in rabbits

Liu, Yang; Dzidotor, Godwin K.; Le, Thinh T.; Morgan, Kristin D.; Curry, Eli; Das, Ritopa; McClinton, Aneesah; Eisenberg, Ellen; Apuzzo, Lorraine N.; Tran, Khanh T. M.; Prasad, Pooja; Flanagan, Tyler J.; Lee, Seok‐Woo; Kan, Ho-Man; Chorsi, Meysam T.; Lo, Kevin W.‐H.; Laurencin, Cato T.; Vinikoor, Tra

doi:10.1126/scitranslmed.abi7282

Cited by 156 publications

(97 citation statements)

References 67 publications

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“…For instance, we previously manufactured a piezoelectric zinc oxide nanogenerator scaffold for enhancing motor recovery and neural function, and implantation of zinc oxide/PCL incorporated treadmill practice provided biomimetic microenvironment and promoted nerve regeneration 18 . A similar design was also applied in the area of articular cartilage repair through PLLA nanofiber-induced piezoelectric effect 174 , further supporting the wide in vivo application of piezoelectric scaffolds. In addition, the study carried out by Chen et al .…”

Section: Biomechanics-based Tissue Engineering Strategies For Pni Man...mentioning

confidence: 74%

Biomechanical microenvironment in peripheral nerve regeneration: from pathophysiological understanding to tissue engineering development

Kong¹,

Gao²,

Qian³

et al. 2022

Theranostics

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Peripheral nerve injury (PNI) caused by trauma, chronic disease and other factors may lead to partial or complete loss of sensory, motor and autonomic functions, as well as neuropathic pain. Biological activities are always accompanied by mechanical stimulation, and biomechanical microenvironmental homeostasis plays a complicated role in tissue repair and regeneration. Recent studies have focused on the effects of biomechanical microenvironment on peripheral nervous system development and function maintenance, as well as neural regrowth following PNI. For example, biomechanical factors-induced cluster gene expression changes contribute to formation of peripheral nerve structure and maintenance of physiological function. In addition, extracellular matrix and cell responses to biomechanical microenvironment alterations after PNI directly trigger a series of cascades for the well-organized peripheral nerve regeneration (PNR) process, where cell adhesion molecules, cytoskeletons and mechanically gated ion channels serve as mechanosensitive units, mechanical effector including focal adhesion kinase (FAK) and yes-associated protein (YAP)/transcriptional coactivator with PDZ-binding motif (TAZ) as mechanotransduction elements. With the rapid development of tissue engineering techniques, a substantial number of PNR strategies such as aligned nerve guidance conduits, three-dimensional topological designs and piezoelectric scaffolds emerge expected to improve the neural biomechanical microenvironment in case of PNI. These tissue engineering nerve grafts display optimized mechanical properties and outstanding mechanomodulatory effects, but a few bottlenecks restrict their application scenes. In this review, the current understanding in biomechanical microenvironment homeostasis associated with peripheral nerve function and PNR is integrated, where we proposed the importance of balances of mechanosensitive elements, cytoskeletal structures, mechanotransduction cascades, and extracellular matrix components; a wide variety of promising tissue engineering strategies based on biomechanical modulation are introduced with some suggestions and prospects for future directions.

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Section: Biomechanics-based Tissue Engineering Strategies For Pni Man...mentioning

confidence: 74%

Biomechanical microenvironment in peripheral nerve regeneration: from pathophysiological understanding to tissue engineering development

Kong¹,

Gao²,

Qian³

et al. 2022

Theranostics

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“…This batteryless electrostimulation could promote protein adsorption and cell migration or recruitment, as well as induce endogenous TGF-β, thereby improving cartilage formation and cartilage regeneration. Rabbits with severe osteochondral defects regenerated hyaline cartilage and achieved complete cartilage healing after 1 to 2 months of self-driven electrical stimulation therapy [93]. This in situ electrical stimulation based on PENGs paves the way for smart scaffold design and future bioelectronic therapies.…”

Section: Cell Traction-driven Pengs For Cell Modulationmentioning

confidence: 92%

“…Both TENGs and PENGs have the advantages of small size, flexibility and good biocompatibility. To date, many wearable and implantable EMNGs have been reported that can efficiently harvest mechanical energy from living organisms, such as from joint motion, heartbeat and respiration, which have laid the foundation for further bioelectronic implants, especially for bones, hearts and muscles with abundant mechanical activities [93,95]. With the current progress and huge development space, EMNGs show tremendous potential for cell modulation and biomedical therapeutics.…”

Section: Summary and Perspectivesmentioning

confidence: 99%

Electromechanical Nanogenerators for Cell Modulation

Liu

Wang

2022

Nanoenergy Advances

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Bioelectricity is an indispensable part of organisms and plays a vital role in cell modulation and tissue/organ development. The development of convenient and bio-safe electrical stimulation equipment to simulate endogenous bioelectricity for cell function modulation is of great significance for its clinical transformation. In this review, we introduce the advantages of an electromechanical nanogenerator (EMNG) as a source of electrical stimulation in the biomedical field and systematically overview recent advances in EMNGs for cell modulation, mainly including cell adhesion, migration, proliferation and differentiation. Finally, we emphasize the significance of self-powered and biomimetic electrostimulation in cell modulation and discuss its challenges and future prospects in both basic research and clinical translation.

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“…Mechanical force from the organism itself (e.g., joint bending and body motions during walking) is a good source to activate piezoelectric polymeric nanofibers in vivo. Piezoelectric nanofibrous webs have been implanted onto the tendon to scavenge mechanical force from joint bending [62,84] or transferred into subcutaneous skin to sense motion force from walking and exercise [85]. It was demonstrated that a 6 μA of current can be detected under cyclic pulling of the mouse leg when the PVDF-TrFE nanofibrous web was placed in the subcutaneous thigh region [85].…”

Section: Cell Traction Forcementioning

confidence: 99%

Electrical Stimulation Enabled via Electrospun Piezoelectric Polymeric Nanofibers for Tissue Regeneration

2022

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Electrical stimulation has demonstrated great effectiveness in the modulation of cell fate in vitro and regeneration therapy in vivo. Conventionally, the employment of electrical signal comes with the electrodes, battery, and connectors in an invasive fashion. This tedious procedure and possible infection hinder the translation of electrical stimulation technologies in regenerative therapy. Given electromechanical coupling and flexibility, piezoelectric polymers can overcome these limitations as they can serve as a self-powered stimulator via scavenging mechanical force from the organism and external stimuli wirelessly. Wireless electrical cue mediated by electrospun piezoelectric polymeric nanofibers constitutes a promising paradigm allowing the generation of localized electrical stimulation both in a noninvasive manner and at cell level. Recently, numerous studies based on electrospun piezoelectric nanofibers have been carried out in electrically regenerative therapy. In this review, brief introduction of piezoelectric polymer and electrospinning technology is elucidated first. Afterward, we highlight the activating strategies (e.g., cell traction, physiological activity, and ultrasound) of piezoelectric stimulation and the interaction of piezoelectric cue with nonelectrically/electrically excitable cells in regeneration medicine. Then, quantitative comparison of the electrical stimulation effects using various activating strategies on specific cell behavior and various cell types is outlined. Followingly, this review explores the present challenges in electrospun nanofiber-based piezoelectric stimulation for regeneration therapy and summarizes the methodologies which may be contributed to future efforts in this field for the reality of this technology in the clinical scene. In the end, a summary of this review and future perspectives toward electrospun nanofiber-based piezoelectric stimulation in tissue regeneration are elucidated.

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Exercise-induced piezoelectric stimulation for cartilage regeneration in rabbits

Abstract: A biodegradable piezoelectric scaffold excited by exercise promotes chondrogenesis and cartilage regeneration in rabbit osteochondral defects.

Cited by 156 publications

References 67 publications

Biomechanical microenvironment in peripheral nerve regeneration: from pathophysiological understanding to tissue engineering development

Biomechanical microenvironment in peripheral nerve regeneration: from pathophysiological understanding to tissue engineering development

Electromechanical Nanogenerators for Cell Modulation

Electrical Stimulation Enabled via Electrospun Piezoelectric Polymeric Nanofibers for Tissue Regeneration

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