Study design: A cross-sectional study with comparison group. Objectives: To examine the effect of rugby training on the blood antioxidant capacity in able-bodied and wheelchair rugby players with tetraplegia. Setting: Poland. Methods: Four groups of subjects participated in the study: sedentary able-bodied males (group SA, n¼19), sedentary males with tetraplegia (group ST, n¼10), able-bodied rugby players (group RA, n¼22) and wheelchair rugby players with tetraplegia (group RT, n¼14). The activities of superoxide dismutase (SOD), glutathione reductase (GR) and catalase (CAT) were determined in erythrocyte hemolysates, whereas glutathione peroxidase (GPX) activity was determined in whole-blood hemolysates. Concentrations of total antioxidant status (TAS) was determined in plasma. Results: SOD activity was significantly higher in the group SA compared with group ST and group RA. No significant differences occurred within the tetraplegic groups: RT and ST. Resting CAT and GPX activities were significantly higher in both the groups of rugby players than in the respective group of sedentary males. There were no differences in GR activity among all the studied groups. Plasma TAS concentration was higher in both the groups of able-bodied males compared with the respective groups of tetraplegics. The present study is the first to conduct an evaluation of wheelchair rugby training-induced adaptations to oxidative stress in individuals with tetraplegia. Conclusion: Adaptive response to training was similar in both able-bodied and wheelchair rugby players, and it was characterized by increased erythrocyte CAT and GPX activities in resting conditions improving resistance to oxidative stress.