Exercise reduced the formation of new adipocytes in the adipose tissue of mice in vivo

Abstract

Exercise has beneficial effects on metabolism and health. Although the skeletal muscle has been a primary focus, exercise also mediates robust adaptations in white adipose tissue. To determine if exercise affects in vivo adipocyte formation, fifty-two, sixteen-week-old C57BL/6J mice were allowed access to unlocked running wheels [Exercise (EX) group; n = 13 males, n = 13 females] or to locked wheels [Sedentary (SED) group; n = 13 males, n = 13 females] for 4-weeks. In vivo adipocyte formation was assessed by the incorporation of deuterium (2H) into the DNA of newly formed adipocytes in the inguinal and gonadal adipose depots. A two-way ANOVA revealed that exercise significantly decreased new adipocyte formation in the adipose tissue of mice in the EX group relative to the SED group (activity effect; P = 0.02). This reduction was observed in male and female mice (activity effect; P = 0.03). Independent analysis of the depots showed a significant reduction in adipocyte formation in the inguinal (P = 0.05) but not in the gonadal (P = 0.18) of the EX group. We report for the first time that exercise significantly reduced in vivo adipocyte formation in the adipose tissue of EX mice using a physiologic metabolic 2H2O-labeling protocol.

Effects of Moderate Intensity Aerobic Exercise to FSTL-1 Regulation in Atherosclerosis: A Systematic Review

“…43 Thus, physical exercises elevate FSTL-1 release from skeletal muscle and prevent metabolism disruption associated with obesity by attenuating adipocyte formation and hepatic steatosis. 44,45 Conclusion FSTL-1 is a relatively new and less studied myokines but probably holds a key role in preventing atherosclerosis progression. Moderate intensity aerobic exercise can be the cornerstone for the FSTL-1-related mechanism leading to the reduction of arterial stiffness, vascular inflammation oxidative stress, and endothelial dysfunction.…”

“…43 Thus, physical exercises elevate FSTL-1 release from skeletal muscle and prevent metabolism disruption associated with obesity by attenuating adipocyte formation and hepatic steatosis. 44 45…”

Scientific Challenges on Theory of Fat Burning by Exercise

“…Approximately 8.4% of subcutaneous abdominal adipocytes are renewed annually with an average half-life of 8.3 years in human adults ( Spalding et al, 2008 ). Abdominal fat mass is determined by the balance of fat cell death and regeneration of adipose tissue, which is influenced by exercise ( Allerton et al, 2021 ). Acute adrenergic stimulation has been reported to induce fat cell death ( Kim et al, 2010 ).…”

Efeitos do treinamento físico intervalado de alta intensidade (HIIT) nas características morfológicas e funcionais do tecido adiposo branco e marrom

“…Outros dados na literatura sugerem que camundongos com livre acesso a roda de corrida podem apresentar redução da adipogênese no TAB-SC, mas não no TAB-PE 137 . Em outro estudo, ratos com livre acesso a roda de corrida não apresentam diferenças no número de adipócitos 138 .…”

“…Tendo em vista essas evidências, o efeito do HIIT no número de adipócitos observado no presente trabalho tem que ser visto com cautela. Primeiramente, porque a metodologia utilizada foi diferente de outros trabalhos [137][138][139][140] . Em segundo lugar, porque marcadores da adipogênese não foram avaliados.…”