Exhalation of H2O2 and thiobarbituric acid reactive substances (TBARs) by healthy subjects

Nowak, Dariusz; Kałucka, Sylwia; Białasiewicz, Piotr; Król, Maciej

doi:10.1016/s0891-5849(00)00457-3

Cited by 152 publications

(150 citation statements)

References 29 publications

Supporting

Mentioning

133

Contrasting

Unclassified

Order By: Relevance

“…EBC H 2 O 2 [ 70 ] and exhaled NO levels [ 71 ] appear to correlate positively with age. Polar VOCs, including aldehydes, appear to be affected by gender, with increased levels in males suggesting metabolic gender differences [ 72 ].…”

Section: Age and Gendermentioning

confidence: 84%

“…There are conflicting data on effects of gender on EBC volume [ 69 ] and H 2 O 2 levels [ 70 ]. EBC H 2 O 2 [ 70 ] and exhaled NO levels [ 71 ] appear to correlate positively with age.…”

Section: Age and Gendermentioning

confidence: 98%

“…In general, EBC concentrations of NO-related compounds [ 80 ], aldehydes [ 81 , 82 ] and total protein may be elevated or remain unchanged with smoking [ 83 ]. Levels of 8-isoprostane, prostaglandin E 2 , leukotriene B 4 [ 84 , 85 ], H 2 O 2 [ 70 ] and TNF-α [ 80 ] increased, whilst levels of IL-1 β [ 80 ], glutathione (an important antioxidant) [ 83 ] and pH [ 76 ] decreased minutes after cigarette smoking. Similarly, increased levels of oxidative markers, including MDA, 8-hydroxydeoxyguanosine, superoxide dismutase and glutathione peroxidise, were identified in the EBC of smokers compared with non-smokers [ 86 ].…”

Section: Smokingmentioning

confidence: 99%

“…One inhalation of salbutamol or ipratropium had no effect on EBC H 2 O 2 and thiobarbituric acid reactive substances (TBARs) concentrations in healthy subjects [ 70 ]. EBC H 2 O 2 and FeNO are decreased in asthmatics following inhaled corticosteroid treatment [ 92 , 93 ], although budesonide administration did not affect EBC nitrite levels in healthy subjects even after inducing an inflammatory response with ozone exposure [ 94 ].…”

Section: Medicationmentioning

confidence: 99%

See 3 more Smart Citations

Exhaled breath condensate: a comprehensive update

Ahmadzai

Huang²,

Hettiarachchi³

et al. 2013

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Since the late 1990s, a surge in interest in the analysis of exhaled breath condensate (EBC) resulted in the American Thoracic Society and European Respiratory Society (ATS/ERS) organising a Task Force in 2001 to develop guidelines on EBC collection and measurement of biomarkers. This Task Force published their guidelines in 2005 based on literature and expert opinions at that time, and multiple shortcomings and knowledge deficits were also identified. The clinical application of EBC collection and its biomarkers are currently still limited by several of these knowledge gaps, hence further guidelines for standardisation are required to ensure external validity. Using related articles produced since the publication of the ATS/ERS Task Force report, this paper attempts to provide a comprehensive update to the original guideline and review the methodological shortcomings identified. This review can hopefully serve as a yardstick for future studies involving this emerging clinical tool.

show abstract

Section: Age and Gendermentioning

confidence: 84%

“…There are conflicting data on effects of gender on EBC volume [ 69 ] and H 2 O 2 levels [ 70 ]. EBC H 2 O 2 [ 70 ] and exhaled NO levels [ 71 ] appear to correlate positively with age.…”

Section: Age and Gendermentioning

confidence: 98%

Section: Smokingmentioning

confidence: 99%

Section: Medicationmentioning

confidence: 99%

See 2 more Smart Citations

Exhaled breath condensate: a comprehensive update

Ahmadzai

Huang²,

Hettiarachchi³

et al. 2013

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…Most of the methods used for hydrogen peroxide detection in the EBC of patients or healthy subjects are based on fluorescence detection. [19][20][21] These are based on the horseradish peroxidase catalyzed reaction of hydrogen peroxide with a suitable substrate to form a fluorescent dimmer. As far as chemiluminescence is concerned, only one method has been developed for hydrogen peroxide detection in EBC.…”

mentioning

confidence: 99%

Hydrogen peroxide assessment in exhaled breath condensate: condensing equipment-rapid flow injection chemiluminescence method

Vasiliou¹,

Makarovska²,

Pneumatikos³

et al. 2007

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Diversas biomoléculas analisadas na Condensação Respiratória Exalada (CRE) podem ser usadas como biomarcadoras, fornecendo informação clínica útil em doenças inflamatórias pulmonares. Peróxido de hidrogênio é um biomarcador especial, pois pode estar relacionado diretamente ao stress oxidativo gerado no corpo humano. Métodos para peróxido de hidrogênio em CRE são essenciais para um grande número de estudos, desde básicos até diagnósticos de doenças. Os métodos usados atualmente não são automatizados, são demorados e cansativos. Além disso, a maioria dos equipamentos de condensação não é portátil. Assim, desenvolvemos um equipamento portátil como uma alternativa extra e um método totalmente automático de injeção em fluxo (FI) com detecção por quimioluminescência para determinação de peróxido de hidrogênio. O equipamento proposto proporciona uma eficiência de coleta superior a 86%, permitindo coletar 2-3 mL de amostra em 20 min. O método envolve uma mistura em fluxo de solução de luminol como carregador da amostra com uma solução de cobalto(II). A reação de CRE com luminol na presença de cobalto(II) como catalisador produz luz proporcional à concentração de peróxido de hidrogênio. As características do método são limite de detecção de 10 nmol L -1 e boa linearidade (r= 0,997). Precisão e recuperações são melhores do que 6,0% RSD e 64,9%, respectivamente. A comparação com o método fluorimétrico manual, comumente usado para CRE, mostrou diferença não significativa, P > 0,001, enquanto a correlação entre os métodos é alta, r=0,94. O método proporciona automatização no manuseio avaliando 180 amostras h -1 e baixo limite de detecção.Several biomolecules in Exhaled Breath Condensate (EBC) can be used as breath biomarkers providing useful clinical information concerning inflammatory lung diseases. Hydrogen peroxide has a special place among biomarkers as it can be directly linked to oxidative stress built up in the human body. Methods for hydrogen peroxide in EBC are essential for a vast number of studies spanning from basic studies to disease diagnosis. Methods currently in use are not automated, timeconsuming and tedious. Moreover, most condensing equipment in use is not portable. To address these, we developed portable condensing equipment as an extra alternative and a fully automated Flow Injection (FI) -Chemiluminescence method for hydrogen peroxide determination. The proposed condensing equipment provides collection efficiency better than 86% allowing the collection of 2-3 mL sample in 20 min. The method involves mixing a luminol solution stream that is used as sample carrier with a cobalt(II) stream. Reaction of EBC with luminol in the presence of cobalt(II) catalyst produces light proportional to hydrogen peroxide concentration. The automated FI method features a 10 nmol L -1 detection limit and good linearity (r= 0.997). Precision and recoveries are better than 6.0% RSD and 64.9%, respectively. Comparison with the, commonly used for EBC, manual fluorimetric method showed no significant difference, P > 0.001, whil...

show abstract

Curcumin reduces oxidative and nitrative DNA damage through balancing of oxidant–antioxidant status in hamsters infected with Opisthorchis viverrini

Pinlaor

Yongvanit

Prakobwong

et al. 2009

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Opisthorchis viverrini (OV) infection is endemic in northeastern Thailand. We have previously reported that OV infection induces oxidative and nitrative DNA damage via chronic inflammation, which contributes to the disease and cholangiocarcinogenesis. Here, we examined the effect of curcumin, an antioxidant, on pathogenesis in OV-infected hamsters. DNA lesions were detected by double immunofluorescence and the hepatic expression of oxidant-generating and antioxidant genes was assessed by quantitative RT-PCR analysis. Dietary 1.0% curcumin significantly decreased OV-induced accumulation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), an oxidative DNA lesion, and 8-nitroguanine, a nitrative DNA lesion, in the nucleus of bile duct epithelial and inflammatory cells. Expression of oxidant-generating genes (inducible nitric oxide synthase; iNOS, its nuclear transcriptional factor, NF-kappaB, and cyclooxygenase-2), and plasma levels of nitrate, malondialdehyde, and alanine aminotransferase, were also decreased in curcumin-treated group. In contrast, curcumin increased the mRNA expression of antioxidant enzymes (Mn-superoxide dismutase and catalase), and ferric-reducing anti-oxidant power in the plasma. In conclusion, curcumin reduced oxidative and nitrative DNA damage by suppression of oxidant-generating genes and enhancement of antioxidant genes, leading to inhibition of oxidative and nitrative stress. Therefore, curcumin may be used as a chemopreventive agent to reduce the severity of OV-associated diseases and the risk of cholangiocarcinoma (CCA).

show abstract

Exhalation of H2O2 and thiobarbituric acid reactive substances (TBARs) by healthy subjects

Cited by 152 publications

References 29 publications

Exhaled breath condensate: a comprehensive update

Exhaled breath condensate: a comprehensive update

Hydrogen peroxide assessment in exhaled breath condensate: condensing equipment-rapid flow injection chemiluminescence method

Curcumin reduces oxidative and nitrative DNA damage through balancing of oxidant–antioxidant status in hamsters infected with Opisthorchis viverrini

Contact Info

Product

Resources

About