Exhaled cysteinyl-leukotrienes and 8-isoprostane in patients with asthma and their relation to clinical severity

Samitas, Konstantinos; Chorianopoulos, D; Vittorakis, Stelios; Ζervas, Εleftherios; Economidou, Erasmia; Papatheodorou, George; Loukides, Stelios; Gaga, Mina

doi:10.1016/j.rmed.2008.11.009

Cited by 76 publications

(67 citation statements)

References 35 publications

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“…Our data confirm a close link between increased oxidative stress and airflow limitation in asthmatic children. This finding is in keeping with previous data reported by our own [7] and other groups [6,10,23], supporting the existence of a correlation between oxidative stress and asthma severity. In a recent study, FITZPATRICK et al [24] demonstrated an imbalance between oxidants and antioxidants, with reduced GSH (reduced glutathione) and increased GSSG (oxidised glutathione) levels in bronchoalveolar lavage of children with severe refractory asthma, supporting a key role for oxidative stress in the pathogenesis of severe asthma.…”

Section: Discussionsupporting

confidence: 93%

“…In many studies, an immunoenzymatic technique (enzymatic immunoassay; EIA) has been used to measure 8-isoprostane in EBC [7][8][9][10]. Though the EIA approach is known to have some weaknesses in the analysis of EBC (inasmuch as the assay works close to its detection limit), to our knowledge no published studies have compared EIA with a reference analytical method based on mass spectrometry in the study of EBC.…”

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confidence: 99%

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EIA and GC/MS analysis of 8-isoprostane in EBC of children with problematic asthma

Carraro

Cogo²,

Isak

et al. 2009

Eur Respir J

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Asthmatic airways are characterised by enhanced oxidative stress, which can be studied by measuring biomarkers, such as 8-isoprostane. The aims of the present study were: 1) to measure the concentrations of 8-isoprostane in exhaled breath condensate (EBC) and urine of children with problematic and well-controlled asthma; 2) to compare the concentrations of 8-isoprostane measured by gas chromatographic/negative ion chemical ionisation mass spectrometry (GC/NICI-MS) and by an enzymatic immunoassay (EIA).We recruited 20 asthmatic allergic children, 13 with well-controlled asthma and seven with problematic asthma. They underwent exhaled nitric oxide measurements and spirometry, and both EBC and urine samples were collected. 8-isoprostane was measured in EBC by GC/NICI-MS and EIA.8-isoprostane concentrations in EBC were significantly higher in children with problematic asthma than in children with well-controlled asthma (p50.01). An acceptable reproducibility emerged between GC/NICI-MS and EIA (coefficient of reproducibility 11.5 pg?mL -1 ). 8-isoprostane levels measured in urine did not correlate with those measured in EBC.We showed that 8-isoprostane in EBC was significantly increased in children with problematic asthma, suggesting a role for oxidative stress in this asthma phenotype. In addition we found an acceptable reproducibility of EIA compared to GC/NICI-MS, even if the latter method had higher accuracy.

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

EIA and GC/MS analysis of 8-isoprostane in EBC of children with problematic asthma

Carraro

Cogo²,

Isak

et al. 2009

Eur Respir J

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show abstract

“…Exhaled breath condensate (EBC) has been increasingly used as a new and non-invasive method to study airway inflammation [2][3][4][5]. EBC has the advantage of being non-invasive and also directly samples mediators from the respiratory tract, thus giving a more direct approach to measuring inflammatory mediators in lung diseases.…”

Section: Introductionmentioning

confidence: 99%

“…EBC contains large number of mediators and their concentrations differ from those found in healthy subjects in several airway diseases, including asthma [2,3,5], chronic obstructive pulmonary disease (COPD) [4], cystic fibrosis (CF) [6,7] and bronchiectasis [8]. Although a research tool at present, EBC may become useful in the diagnosis of patients with various pulmonary diseases in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Correlation between Eicosanoids in Bronchoalveolar Lavage Fluid and in Exhaled Breath Condensate

et al. 2011

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Abstract. Exhaled breath condensate (EBC) has been increasingly used as a new and non-invasive method to study airway inflammation. In this study we have compared the concentrations of lipid mediators in EBC with concentrations in bronchoalveolar lavage fluid (BALF). We included 37 patients undergoing bronchoscopy (12 sarcoidosis, 12 COPD, 6 lung cancer, 5 chronic cough, 1 Wegener's granulomatosis, 1 sclerodermia). Patients were not allowed to have exacerbation or any change in concomitant medication for at least 4 weeks prior to the study. In all patients, EBC was collected immediately prior to the bronchoscopy. The levels of cys-LTs, LTB4, 8-isoprostane were significantly higher in BALF compared to EBC (p < 0.0001, p < 0.001, p < 0.0001 for cys-LTs, LTB4, 8-isoprostane respectively). Moreover, there was a strong positive correlation between both leukotriene B4 and 8-isoprostane in BALF and EBC (r = 0.53 and r = 0.79, p < 0.01, respectively) in patients with sarcoidosis and COPD but there was no correlation between eicosanoids BALF and EBC in patients with chronic cough and lung cancer. This is the first study to compare EBC and BALF in different lung diseases which demonstrated significant correlations between the levels of eicosanoids in BALF and EBC in patients with COPD and sarcoidosis. EBC may be useful in measuring inflammation in several inflammatory lung diseases.

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“…The collection of EBC is performed by cooling exhaled air during spontaneous respiration (Kostikas et al 2008 This new approach -using genotyping to identify a subset of patients with asthma who respond to a specifi c therapy -can have far-reaching implications in terms of asthma outcomes. higher concentrations of leukotrienes in EBC than mild asthmatics and controls (Samitas et al 2009). Metabolomic analysis identifies potential biomarkers for a disease by analyzing the products of cell metabolism using mass spectrometry or high-resolution proton nuclear magnetic resonance spectroscopy (¹H-NMR).…”

Section: Can Exhaled Breath Help?mentioning

confidence: 99%

Asthma patients with specific genotypes identified for fish oil treatment trial

Allayee¹,

Davis²,

Fortenko³

et al. 2011

Cal Ag

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The lifetime prevalence of asthma in California is nearly 20%, and better therapies are needed to manage this common chronic disease. Fish oils containing omega-3 fatty acids are considered a potential therapy for asthma and other inflammatory diseases. Fish oil inhibits the production of arachidonic acid 5-lipoxygenase (ALOX5), an enzyme that exacerbates the lung inflammation that causes asthma. We discuss the genetics of asthma and our preliminary results using a strategy to identify the subgroup of patients who may respond well to treatment with fish oil. These findings, and others, suggest that certain gene polymorphisms of the ALOX5 gene predispose patients to the increased production of inflammatory leukotrienes. Our clinical trials will test the hypothesis that patients with moderate to severe asthma, and with specific high-risk ALOX5 gene sequence variations, will have fewer asthma symptoms when treated with fish oil. The strategy is to decrease the total burden of leukotriene production by supplementing with omega-3 polyunsaturated fatty acids. These studies will also help determine whether genotyping or metabolic profiling (for example, with exhaled breath condensate) can help establish "personalized medicine" for asthma.

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Exhaled cysteinyl-leukotrienes and 8-isoprostane in patients with asthma and their relation to clinical severity

Abstract: 8-isoprostane and cys-LT are detectable in EBC of healthy subjects and their levels progressively increase in asthmatic patients according to disease severity. The correlation found between these two lipid mediators indicating a link between oxidative stress and airway inflammation.

Cited by 76 publications

References 35 publications

EIA and GC/MS analysis of 8-isoprostane in EBC of children with problematic asthma

EIA and GC/MS analysis of 8-isoprostane in EBC of children with problematic asthma

Correlation between Eicosanoids in Bronchoalveolar Lavage Fluid and in Exhaled Breath Condensate

Asthma patients with specific genotypes identified for fish oil treatment trial

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