Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin

Prosser, Derek C.; Drivas, Theodore G.; Maldonado-Báez, Lymarie; Wendland, Beverly

doi:10.1083/jcb.201104045

Cited by 71 publications

(125 citation statements)

References 72 publications

(111 reference statements)

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“…Dynamin and RhoA-dependent raft endocytosis has been reported for IL2 internalization in T cells and the compensatory endocytosis of bladder umbrella cells (Khandelwal et al, 2010;Lamaze et al, 2001). Existence of a non-clathrin, RhoA-dependent endocytic route in yeast suggests that this pathway may represent a conserved endocytic pathway (Prosser et al, 2011). The demonstration here that AMF endocytosis is Rac1-, and not RhoA-, dependent defines the dynamin-dependent, Rac1-PI3K raft endocytic route followed by AMF to the ER as a distinct raft endocytosis pathway.…”

Section: Amf Inhibits Gp78-dependent Mitofusin Degradation and Mitochmentioning

confidence: 99%

Raft endocytosis of autocrine motility factor regulates mitochondrial dynamics via rac1 signaling and the gp78 ubiquitin ligase

Shankar

Kojic

St-Pierre

et al. 2013

Journal of Cell Science

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SummaryGp78 is a cell surface receptor that also functions as an E3 ubiquitin ligase in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway. The Gp78 ligand, the glycolytic enzyme phosphoglucose isomerase (PGI; also called autocrine motility factor, AMF), functions as a cytokine upon secretion by tumor cells. AMF is internalized through a PI3K-and dynamin-dependent raft endocytic pathway to the smooth ER; however, the relationship between AMF and Gp78 ubiquitin ligase activity remains unclear. AMF uptake to the smooth ER is inhibited by the dynamin inhibitor, dynasore, is reduced in Gp78 knockdown cells and induces the dynamin-dependent downregulation of its cell surface receptor. AMF uptake is Rac1-dependent and is inhibited by expression of dominant-negative Rac1 and the Rac1 inhibitor NSC23766, and is therefore distinct from Cdc42-and RhoA-dependent raft endocytic pathways. AMF stimulates Rac1 activation, but this is reduced by dynasore treatment and is absent in Gp78-knockdown cells; therefore, AMF activities require Gp78-mediated endocytosis. AMF also prevents Gp78-induced degradation of the mitochondrial fusion proteins, mitofusin 1 and 2 in a dynamin-, Rac1-and phosphoinositide 3-kinase (PI3K)-dependent manner. Gp78 induces mitochondrial clustering and fission in a manner dependent on GP78 ubiquitin ligase activity, and this is also reversed by uptake of AMF. The raft-dependent endocytosis of AMF, therefore, promotes Rac1-PI3K signaling that feeds back to promote AMF endocytosis and also inhibits the ability of Gp78 to target the mitofusins for degradation, thereby preventing Gp78-dependent mitochondrial fission. Through regulation of an ER-localized ubiquitin ligase, the raft-dependent endocytosis of AMF represents an extracellular regulator of mitochondrial fusion and dynamics.

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Section: Amf Inhibits Gp78-dependent Mitofusin Degradation and Mitochmentioning

confidence: 99%

Raft endocytosis of autocrine motility factor regulates mitochondrial dynamics via rac1 signaling and the gp78 ubiquitin ligase

Shankar

Kojic

St-Pierre

et al. 2013

Journal of Cell Science

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“…To do this, we used a previously established assay of clathrin-dependent endocytosis based on the clathrin adaptor-mediated internalization of Mup1-GFP, a methionine permease (15,18). In control cells (WT and ⌬4-pENT1), within 30 min of glucose starvation Mup1-GFP cell surface levels decreased, and Mup1-GFP appeared in internal structures ( Fig.…”

Section: Plasma Membrane To Vacuole Traffic Is Required For Cell Survmentioning

confidence: 99%

Glucose Starvation Inhibits Autophagy via Vacuolar Hydrolysis and Induces Plasma Membrane Internalization by Down-regulating Recycling

Lang

Martínez-Márquez

Prosser

et al. 2014

Journal of Biological Chemistry

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107

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Background: During energy starvation, cells utilize intracellular resources for survival; however, the resources used during glucose starvation are unknown. Results: In glucose-starved yeast, vacuolar hydrolysis and endocytosis promote survival whereas autophagy is dispensable. Conclusion: Vacuolar hydrolysis blocks autophagy and provides resources for survival during glucose starvation. Significance: This new survival mechanism could protect cells from starvation in many situations.

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“…The major route into the cell is mediated by clathrin, although it is interesting to note that a clathrin-independent endocytosis (CIE) pathway in S. cerevisiae has been found (Prosser et al, 2011) and is involved in a specific form of α-arrestin-regulated receptor internalization (Prosser et al, 2015). Budding yeast is amenable to molecular genetic manipulations, making it possible to genetically tag proteins of interest with fluorescent proteins in order to study their localization and dynamics at endogenous levels.…”

Section: Introductionmentioning

confidence: 99%

Clathrin-mediated endocytosis in budding yeast at a glance

Drubin

Sun

2016

Journal of Cell Science

115

105

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Clathrin-mediated endocytosis is an essential cellular process that involves the concerted assembly and disassembly of many different proteins at the plasma membrane. In yeast, live-cell imaging has shown that the spatiotemporal dynamics of these proteins is highly stereotypical. Recent work has focused on determining how the timing and functions of endocytic proteins are regulated. In this Cell Science at a Glance article and accompanying poster, we review our current knowledge of the timeline of endocytic site maturation and discuss recent works focusing on how phosphorylation, ubiquitylation and lipids regulate various aspects of the process.

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Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin

Abstract: Much like mammalian cells, yeast contain a Rho-dependent pathway for endocytosis in addition to canonical clathrin-dependent endocytosis.

Cited by 71 publications

References 72 publications

Raft endocytosis of autocrine motility factor regulates mitochondrial dynamics via rac1 signaling and the gp78 ubiquitin ligase

Raft endocytosis of autocrine motility factor regulates mitochondrial dynamics via rac1 signaling and the gp78 ubiquitin ligase

Glucose Starvation Inhibits Autophagy via Vacuolar Hydrolysis and Induces Plasma Membrane Internalization by Down-regulating Recycling

Clathrin-mediated endocytosis in budding yeast at a glance

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