1982
DOI: 10.1016/0309-1651(82)90039-x
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Exit of human diploid cells from low serum quiescence

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Cited by 14 publications
(5 citation statements)
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“…It would be interesting to perform similar studies in cells which can be density arrested even in the presence of optimal concentrations of growth factors. A kinetic study using such a system has been reported by Duncan et al (1982). These investigators showed that normal diploid fibroblasts maintained and refed twice weekly with 0.5% FBS (and therefore density dependent growth arrested but not completely growth factor deprived) for 21 days did not exhibit a delay in entry into S upon stimulation, whereas cells which were maintained in their original plating medium (and therefore density arrested and growth factor deprived) did exhibit a delay.…”
Section: Discussionmentioning
confidence: 99%
“…It would be interesting to perform similar studies in cells which can be density arrested even in the presence of optimal concentrations of growth factors. A kinetic study using such a system has been reported by Duncan et al (1982). These investigators showed that normal diploid fibroblasts maintained and refed twice weekly with 0.5% FBS (and therefore density dependent growth arrested but not completely growth factor deprived) for 21 days did not exhibit a delay in entry into S upon stimulation, whereas cells which were maintained in their original plating medium (and therefore density arrested and growth factor deprived) did exhibit a delay.…”
Section: Discussionmentioning
confidence: 99%
“…The recovery of some cells to greater than control values of precursor incorporation is due to a n "overshoot" and has been previously described deprivation. Recently, the findings of Augenlicht and Baserga (1974) were not reproduced by Duncan et al (1982) utilizing a slightly different experimental protocol involving weekly refeeding of the serum-deprived cultures. Our results are more consistent with the notion that qualitative differences do exist between cultures that are maintained for prolonged (greater than 3week) periods in quiescence, and those that have been noncycling for shorter periods of time.…”
Section: Discussionmentioning
confidence: 99%
“…days after subcultivation will yield the variant ratios for S phase cells, while inclusion of HU at this time point will inhibit DNA synthesis and yield basal or G1 variant ratios. Confluent populations have been described as being in either the G1 or the GO state of the cell cycle (Augenlicht and Baserga, 19743, while quiescent populations incubated with 0.5% serum are described as being in GO (Duncan et al, 1982).…”
Section: Discussionmentioning
confidence: 99%