EXOC1 regulates cell morphology of spermatogonia and spermatocytes in mice

Abstract

31Spermatogenesis requires high regulation of germ cell morphology. The 32 spermatogonia regulates its differentiation state by its own migration. The male 33 germ cells differentiate and mature with the formation of syncytia, failure of 34 forming the appropriate syncytia results in the arrest of spermatogenesis at the 35 spermatocyte stage. However, the detailed molecular mechanisms of male germ 36 cell morphological regulation are unknown. Here, we found that EXOC1 is 37 important for the pseudopod formation of spermatogonia and spermatocyte 38 syncytia in mice. We found that while EXOC1 contributes to the inactivation of 39Rac1 in the pseudopod formation of spermatogonia, in spermatocyte syncytium 40 formation, EXOC1 and SNAP23 cooperate with STX2. Our results showed that 41 EXOC1 functions in concert with various cell morphology regulators in 42 spermatogenesis. Since EXOC1 is known to bind to several cell morphogenesis 43 factors, this study is expected to be the starting point for the discovery of many 44 morphological regulators of male germ cells.

Multiplex genotyping method to validate the multiallelic genome editing outcomes using machine learning-assisted long-read sequencing

“…S14c,d). Furthermore, we generated KO mice for the Stx2 gene using the two-cut strategy to cleave 727 bp, including exon 5 of Stx2 (42). DAJIN reported that 13 of the 29 founder mice (BC01, BC03, BC04, BC05, BC07, BC09, BC14, BC15, BC20, BC21, BC22, BC23, and BC24) had the 'Mutated Del' allele (Additional file 2: Fig.…”