Exocytosis of nanoparticles from cells: Role in cellular retention and toxicity

Sakhtianchi, Ramin; Minchin, Rodney F.; Lee, Ki‐Bum; Alkilany, Alaaldin M.; Serpooshan, Vahid; Mahmoudi, Morteza

doi:10.1016/j.cis.2013.10.013

Cited by 231 publications

(215 citation statements)

References 166 publications

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“…Transient loading of cells requires the release of the NPs, as we have described using the physics model. NP release has been reported before in multiple cell types and reviewed by Sakhtianchi et al and Oh et al 32,25 Internalized NPs are often delivered in early endosomes, which are considered as the main sorting station of the endocytic machinery. 33,34 Although the majority of the NPs reside in the endolysosomal pathways, a fraction of the NPs is capable to escape to recycling endosomes and is eventually released by cells.…”

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confidence: 99%

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Transient loading of CD34<sup>+</sup> hematopoietic progenitor cells with polystyrene nanoparticles

Deville¹,

Hadiwikarta²,

Smisdom³

et al. 2017

IJN

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confidence: 99%

“…39 Also NPs that are present in the cytoplasm (eg, NP uptake by diffusion or endolysosomal escape of NPs) can leave the cell through re-entry to the vesicular system or via unspecific mechanisms. 32,37 Based on these reports, it is conceivable to have also an active release in HPCs.…”

mentioning

confidence: 99%

Transient loading of CD34<sup>+</sup> hematopoietic progenitor cells with polystyrene nanoparticles

Deville¹,

Hadiwikarta²,

Smisdom³

et al. 2017

IJN

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“…Only a slight increase of solution DOX level is observed upon incubating the FSFSMs at pH 8 between 0 and 21 h, reflecting the exocytosis of sperm cells. [44] In contrast, the DOX concentration increases dramatically during the first 4 h in acidic environment and reaches a plateau afterward, indicating the immediate sperm death triggered by the pH change. At lower pH, the hydrogen bonding between DOX and amine-functionalized Fe 2 O 3 NPs breaks apart due to the protonation of the amine group, contributing to the increase of the DOX solution concentration.…”

Section: Doi: 101002/adbi201700160mentioning

confidence: 99%

Chemotactic Guidance of Synthetic Organic/Inorganic Payloads Functionalized Sperm Micromotors

et al. 2017

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The preparation and operation of free swimming functionalized sperm micromotors (FSFSMs) as intelligent self‐guided biomotors with intrinsic chemotactic motile behavior are reported. The natural sperm biomotors are functionalized with a wide variety of synthetic nanoscale payloads, such as CdSe/ZnS quantum dots, doxorubicin hydrochloride drug coated iron‐oxide nanoparticles, and fluorescein isothiocyanate‐modified Pt nanoparticles via endocytosis. The FSFSMs display efficient self‐propulsion in various biological and environmental media with controllable swarming behavior upon exposure to a chemical attractant. As a new class of environmentally responsive smart biomotors, the control of the FSFSM speed is achieved by varying the solution osmolarity that leads to different flagellar lengths. High drug loading capacity and responsive release kinetics are obtained with such sperm biomotors. The transport of synthetic cargo can be guided by the intrinsic chemotaxis of the FSFSMs. The chemotactic characteristics, speed control mechanism, and responsive payload release of the FSFSMs are investigated. Such use of free swimming functionalized sperm cells as intelligent microscale biomotors offers considerable potential for diverse biomedical and environmental applications.

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“…It was previously shown that different nanoparticle properties, such as size, shape, material, and surface coating, as well as cell type, interaction with other cells, and cellular environment, influence nanoparticle uptake and cellular behavior [4][5][6][7][8]. Therefore, understanding the interactions between nanoparticles and cells within a physiological cell environment is important for the cellular uptake of drug and drug delivery systems.…”

Section: Introductionmentioning

confidence: 99%

Effects of Biomimetic Shear Stress by Self-Assembled Gelatin Oleic Nanoparticles Containing Paclitaxel on the Behavior of Cancer Cell Lines in a Microfluidic System

Kang¹,

Tran²,

Oh³

et al. 2017

Preprint

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Fluid flow in human body is generally known to influence a variety of cellular behaviors. Different nanoparticle properties as well as cell type, interaction with other cells and cellular environments also show significant effect on nanoparticle uptake and drug efficacy. The aim of this study was to evaluate the effect of shear stress on cellular behaviors of biocompatible and biodegradable nanoparticles to cancer cells (A549 cell lines) in a biomimetic microfluidic system. We prepared a gelatin-oleic conjugate (GOC) as an amphiphilic biomaterial to prepare self-assembled gelatin-oleic nanoparticles (GON). Coumarin-6 and paclitaxel were used as the fluorescence marker and model drug, respectively, and were loaded into GONs by incubation (C-GONs; PTX-GONs). Additionally, we evaluated the cellular uptake of fluorescence labeled C-GONs and the drug efficacy of PTX-GONs. The cellular uptake of C-GONs by A549 cells in the absence of shear stress revealed that the mean fluorescence intensity was slightly decreased compared to that in the presence of shear stress. The results also indicated that negatively charged PTX-GONs had a lower cancer killing effect under dynamic conditions than that under static conditions. It also suggested that fluidic shear stress did not significantly affect drug uptake and efficiency in case of PTX-GONs. The cellular interactions between nanoparticles and cells in drug delivery should be carefully examined according to the physicochemical properties of nanoparticles such as the type of materials, size and mainly surface charge in a biomimetic microfluidic condition.

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Exocytosis of nanoparticles from cells: Role in cellular retention and toxicity

Cited by 231 publications

References 166 publications

Transient loading of CD34<sup>+</sup> hematopoietic progenitor cells with polystyrene nanoparticles

Transient loading of CD34<sup>+</sup> hematopoietic progenitor cells with polystyrene nanoparticles

Chemotactic Guidance of Synthetic Organic/Inorganic Payloads Functionalized Sperm Micromotors

Effects of Biomimetic Shear Stress by Self-Assembled Gelatin Oleic Nanoparticles Containing Paclitaxel on the Behavior of Cancer Cell Lines in a Microfluidic System

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Exocytosis of nanoparticles from cells: Role in cellular retention and toxicity

Cited by 231 publications

References 166 publications

Transient loading of CD34<sup>+</sup>&nbsp;hematopoietic progenitor cells with polystyrene nanoparticles

Transient loading of CD34<sup>+</sup>&nbsp;hematopoietic progenitor cells with polystyrene nanoparticles

Chemotactic Guidance of Synthetic Organic/Inorganic Payloads Functionalized Sperm Micromotors

Effects of Biomimetic Shear Stress by Self-Assembled Gelatin Oleic Nanoparticles Containing Paclitaxel on the Behavior of Cancer Cell Lines in a Microfluidic System

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Transient loading of CD34<sup>+</sup> hematopoietic progenitor cells with polystyrene nanoparticles

Transient loading of CD34<sup>+</sup> hematopoietic progenitor cells with polystyrene nanoparticles