Exogenous 24-epibrassinolide alleviates chilling injury in peach fruit through modulating PpGATA12-mediated sucrose and energy metabolisms

Hu, Shunqing; Hou, Yuanyuan; Zhao, Liangyi; Zheng, Yonghua; Jin, Peng

doi:10.1016/j.foodchem.2022.133996

Cited by 35 publications

(6 citation statements)

References 36 publications

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“…Additionally, when exogenous EBR and JA treatments were applied to grape berries, the expression levels of the genes involved in lipid metabolism (DNL, VIT_13s0019g04580 and PK, VIT_08s0007g04170 at DAA 30 and 80), amino acid metabolism (PEPCase, VIT_19s0014g01390 at DAA 50 to 80), and tricarboxylic acid regulation (CSY, VIT_12s0142g00610 at DAA 80) were significantly higher. Hu et al (2022) also discovered that the PpGATA12 gene was greatly influenced by exogenous EBR and regulated peach fruit's energy and sucrose metabolisms. And exogenous MeJA increased soluble solids in tomato fruit by activating sucrose phosphate synthase (SPS) and sucrose synthase (SUS) activities (Li et al, 2021b).…”

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confidence: 99%

Improving berry quality and antioxidant ability in ‘Ruidu Hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones

Javed²,

Wu³

et al. 2022

Front. Plant Sci.

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Grape berries contain a variety of metabolites, such as anthocyanins, sugars, fatty acids, and antioxidants. Endogenous phytohormones strongly influence these metabolites, which regulate berry quality improvement. In this study, we evaluated the effects of 2,4-epibrassinolide (EBR, brassinolide (BR)-like growth regulator), jasmonic acid (JA), and their signaling inhibitors brassinazole (Brz), and sodium diethyldithiocarbamate (DIECA) on berry quality and antioxidant ability. Overall, the pre-harvest application of 0.5 mg L-1 EBR and 100 μmol L-1 JA significantly influences the quality of the grape berry. Results showed that EBR was superior to other treatments at enhancing the content of different metabolites, including anthocyanins, fructose, glucose, and a variety of fatty acids, in grapes. EBR and JA also enhanced the synthesis of gibberellin3 (GA3), cytokinin (CTK), salicylic acid (SA), JA, methyl jasmonate (MeJA), BR, and abscisic acid (ABA), while inhibiting the synthesis of auxin (IAA). Most genes related to BR/JA and anthocyanins/sugars/fatty acids biosynthesis were up-regulated. The effects of Brz and DIECA on the grape berry quality were totally reversed throughout the study, as shown by EBR and JA. According to correlation analysis, EBR and JA have a beneficial positive interaction that promotes the formation of strong coherences in grape berries between ABA/IAA/ZT-fruit expansion, BR/JA/MeJA/GA3/ZR-biochemical characteristics development, JA/MeJA/ABA/GA3/SA/ZR-antioxidant capacity enhancement, and JA/MeJA/IAA/GA3/ZT/ZR-fatty acids accumulation. In this regard, we concluded that preharvest exogenous 0.5 mg L-1 EBR and 100 μmol L-1 JA is a successful way to improve grape berry quality.

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confidence: 99%

Improving berry quality and antioxidant ability in ‘Ruidu Hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones

Javed²,

Wu³

et al. 2022

Front. Plant Sci.

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“…Energy deficits can impair the integrity of cell membranes and lead to a decline in the post-harvest quality of horticultural products (Wang et al, 2020c;Li et al, 2021a). ATP and ADP contents in loquats (Jin et al, 2015), kiwifruit (Wang et al, 2020b), and peaches (Hu et al, 2023), have been reported to drop rapidly and remain at low levels during cold storage. In this study, ATP, ADP, and energy charge declined rapidly in cucumber fruits during cold storage, and the symptoms of fruit CI were aggravated.…”

Section: Discussionmentioning

confidence: 99%

“…CCO provides energy to cells mainly through oxidative phosphorylation and is a key enzyme in the oxidative phosphorylation process in the mitochondrial respiratory chain (Millar et al, 1995). Cold stress affects the production of ATP by affecting mitochondrial structure and the activity of these enzymes, and the loss of ATP impairs metabolism and destroys cell structure; this further aggravates CI, resulting in a decline in the quality of horticultural products (Jin et al, 2015;Wang et al, 2020b;Hu et al, 2023). Altogether together, the results of this study demonstrate that fucoidan treatment can maintain mitochondrial function by improving the activities of relevant enzymes in cucumbers during cold storage, thereby ensuring continual energy synthesis and ultimately reducing the occurrence of CI.…”

Section: Discussionmentioning

confidence: 99%

Fucoidan treatment alleviates chilling injury in cucumber by regulating ROS homeostasis and energy metabolism

et al. 2022

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IntroductionChilling injury is a major hindrance to cucumber fruit quality during cold storage.Methods and ResultsIn this study, we evaluated the effects of fucoidan on fruit quality, reactive oxygen species homeostasis, and energy metabolism in cucumbers during cold storage. The results showed that, compared with the control cucumber fruit, fucoidan-treated cucumber fruit exhibited a lower chilling injury index and less weight loss, as well as reduced electrolyte leakage and malondialdehyde content. The most pronounced effects were observed following treatment with fucoidan at 15 g/L, which resulted in increased 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radical scavenging rates and reduced superoxide anion production rate and hydrogen peroxide content. The expression and activity levels of peroxidase, catalase, and superoxide dismutase were enhanced by fucoidan treatment. Further, fucoidan treatment maintained high levels of ascorbic acid and glutathione, and high ratios of ascorbic acid/dehydroascorbate and glutathione/oxidized glutathione. Moreover, fucoidan treatment increased the activities of ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase and their gene expression. Fucoidan treatment significantly delayed the decrease in ATP and ADP, while preventing an increase in AMP content. Finally, fucoidan treatment delayed the decrease of energy charge and the activities and gene expression of H+-ATPase, Ca2+-ATPase, cytochrome c oxidase, and succinate dehydrogenase in cucumber fruits.ConclusionAltogether, our findings indicate that fucoidan can effectively enhance antioxidant capacity and maintain energy metabolism, thereby improving cucumber cold resistance during cold storage.

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“…The reason for chilling injury is the formation of ice crystals in plant tissue leading to cell dehydration, membrane leakage, and respiratory dysfunction [ 26 ]. 2,4-epibrassinolide improved the chilling tolerance of peach fruit by regulating PpCBF5-mediated membrane lipid metabolism and PpGATA12-mediated sucrose and energy metabolisms [ 27 , 28 ]. Chen et al [ 29 ] reported that 2,4-epibrassinolide enhanced the chilling tolerance of loquat fruit by modulating cell walls and membrane fatty acid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Brassinolide Soaking Reduced Nitrite Content and Extended Color Change and Storage Time of Toona sinensis Bud during Low Temperature and Near Freezing-Point Temperature Storage

Guo

et al. 2022

IJMS

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Low temperatures are often used to preserve fruits and vegetables. However, low-temperature storage also causes problems, such as chilling injury, nitrite accumulation, and browning aggravation in plants. This study investigated the effects of brassinolide (BR,1.0 mg L−1) solution soaking, storage temperatures (−2 ± 0.5 °C, 4 ± 0.5 °C, and 20 ± 1 °C), and their combinations on nitrite content, color change, and quality of stored Toona sinensis bud. The results showed that low temperature (LT, 4 ± 0.5 °C) and near freezing-point temperature (NFPT, −2 ± 0.5 °C) storage effectively inhibited the decay of T. sinensis bud compared to room temperature (20 ± 1 °C, the control). The combined treatments of BR with LT or NFPT reduced nitrite content and maintained the color and the contents of vitamin C, carotenoids, saponins, β-sitosterol, polyphenol, anthocyanin, flavonoids, and alkaloids in T. sinensis bud. BR soaking delayed the occurrence of chilling injury during NFPT storage. Meanwhile, BR soaking enhanced the DPPH radical scavenging activity, ABTS activity, and FRAP content by increasing SOD and POD activity and the contents of proline, soluble, and glutathione, thus decreasing MDA and hydrogen peroxide content and the rate of superoxide radical production in T. sinensis bud during NFPT storage. This study provides a valuable strategy for postharvest T. sinensis bud in LT and NFPT storage. BR soaking extended the shelf life during LT storage and maintained a better appearance and nutritional quality during NFPT storage.

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Exogenous 24-epibrassinolide alleviates chilling injury in peach fruit through modulating PpGATA12-mediated sucrose and energy metabolisms

Cited by 35 publications

References 36 publications

Improving berry quality and antioxidant ability in ‘Ruidu Hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones

Improving berry quality and antioxidant ability in ‘Ruidu Hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones

Fucoidan treatment alleviates chilling injury in cucumber by regulating ROS homeostasis and energy metabolism

Brassinolide Soaking Reduced Nitrite Content and Extended Color Change and Storage Time of Toona sinensis Bud during Low Temperature and Near Freezing-Point Temperature Storage

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