Exogenous acquisition of Pseudomonas aeruginosa in intensive care units: a prospective multi-centre study (DYNAPYO study)

Coppry, Maïder; Leroyer, Christophe; Saly, Marion; Venier, A.G.; Slekovec, C.; Bertrand, Xavier; Parer, Sylvie; Alfandari, S.; Cambau, Emmanuelle; Mégarbane, Bruno; Lawrence, Christine; Clair, Bernard; Lepape, Alain; Cassier, Pierre; Trivier, Dominique; Boyer, Alexandre; Boulestreau, H.; Asselineau, Julien; Dubois, Véronique; Thiébaut, Rodolphe; Rogues, A.-M.

doi:10.1016/j.jhin.2019.08.008

Cited by 14 publications

(10 citation statements)

References 38 publications

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“…In ICUs, these infections are generally considered endogenous (i.e., coming from the pre-existing colonization of patients). Some studies, however, showed that the clinical strains of P. aeruginosa were genetically related to the strains found in the patient's room, e.g., in tap water, sinks, and hand wash stations ( Coppry et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…The new Drinking Water Directive (DWD) expected to come into force before December 2020 will introduce a new risk-based approach including an assessment of the possible risks stemming from the domestic distribution systems, including Legionella by the Member State (“domestic distribution risk assessment”). Poorly managed water networks may be vulnerable to microorganisms, including Legionella and Pseudomonas aeruginosa , which can infect hospitalized patients who already have underlying conditions, immunosuppression and use invasive devices ( Coppry et al, 2020 ; Montagna et al, 2016 , 2018 ). Thus, water stagnation in unused buildings (e.g.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of lockdown on the microbiological status of the hospital water network during COVID-19 pandemic

Giglio

Diella

Lopuzzo

et al. 2020

Environmental Research

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The COVID-19 pandemic started in China in early December 2019, and quickly spread around the world. The epidemic gradually started in Italy at the end of February 2020, and by May 31, 2020, 232,664 cases and 33,340 deaths were confirmed. As a result of this pandemic, the Italian Ministerial Decree issued on March 11, 2020, enforced lockdown; therefore, many social, recreational, and cultural centers remained closed for months. In Apulia (southern Italy), all non-urgent hospital activities were suspended, and some wards were closed, with a consequent reduction in the use of the water network and the formation of stagnant water. This situation could enhance the risk of exposure of people to waterborne diseases, including legionellosis. The purpose of this study was to monitor the microbiological quality of the water network (coliforms, E. coli , Enterococci, P. aeruginosa, and Legionella ) in three wards (A, B and C) of a large COVID-19 regional hospital, closed for three months due to the COVID-19 emergency. Our study revealed that all three wards' water network showed higher contamination by Legionella pneumophila sg 1 and sg 6 at T1 (after lockdown) compared to the period before the lockdown (T0). In particular, ward A at T1 showed a median value = 5600 CFU/L (range 0–91,000 CFU/L) vs T0, median value = 75 CFU/L (range 0–5000 CFU/L) ( p-value = 0.014); ward B at T1 showed a median value = 200 CFU/L (range 0–4200 CFU/L) vs T0, median value = 0 CFU/L (range 0–300 CFU/L) ( p-value = 0.016) and ward C at T1 showed a median value = 175 CFU/L (range 0–22,000 CFU/L) vs T0, median value = 0 CFU/L (range 0–340 CFU/L) ( p-value < 0.001). In addition, a statistically significant difference was detected in ward B between the number of positive water samples at T0 vs T1 for L. pneumophila sg 1 and sg 6 (24% vs 80% p-value < 0.001) and for coliforms (0% vs 64% p-value < 0.001). Moreover, a median value of coliform load resulted 3 CFU/100 ml (range 0–14 CFU/100 ml) at T1, showing a statistically significant increase versus T0 (0 CFU/100 ml) ( p-value < 0.001). Our results highlight the need to implement a water safety plan that includes staff training and a more rigorous environmental microbiological surveillance in all hospitals before occupying a closed ward for a longer than one week, according to national and international guidelines.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impact of lockdown on the microbiological status of the hospital water network during COVID-19 pandemic

Giglio

Diella

Lopuzzo

et al. 2020

Environmental Research

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“…Intubated and immunocompromised patients are especially vulnerable to this common pathogen ( 6 , 7 ). When a prospective multicenter study of P. aeruginosa infections in ICUs was conducted in France (the DYNAPYO cohort), 386 out of 1,561 (24.73%) patients who were not infected with P. aeruginosa at admission were found to have acquired the pathogen during their hospitalization ( 8 ). In addition, a Canadian surveillance study of over 8,000 ICU patients between 2007 and 2016 (CANWARD surveillance study) reported that P. aeruginosa accounted for 10.6% of all isolates, thereby ranking it second among microbial taxa ( 9 ).…”

Section: Introductionmentioning

confidence: 99%

Time Series Genomics of Pseudomonas aeruginosa Reveals the Emergence of a Hypermutator Phenotype and Within-Host Evolution in Clinical Inpatients

Liu¹,

Yang²,

Chen³

et al. 2022

Microbiol Spectr

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“…Tap water (Garvey et al., 2016; Gautam, 2021) and medical equipment (Alvarez‐Lerma et al., 2018) are often contaminated with P. aeruginosa which not only has innate resistance to a variety of antibiotics, it also has the propensity to develop drug resistance (Feng et al., 2019; Langendonk et al., 2021). P. aeruginosa accounts for 10%–15% of all nosocomial infections (Bassetti et al., 2018), especially those in intensive care unit (ICU) (Cohen et al., 2017; Coppry et al., 2020). Its pathogenic characteristics cause secondary infections, occurring mostly when the body's immunity is reduced, such as after extensive burns (Karaky et al., 2020) and following surgery (Richards & Marshall, 2018).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Pseudomonas aeruginosa using a DNAzyme‐based sensor

Qin

Fan

et al. 2021

Food Science & Nutrition

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In the present study, a DNAzyme was screened in vitro through the use of a DNA library and crude extracellular mixture (CEM) of Pseudomonas aeruginosa. Following eight rounds of selection, a DNAzyme termed PAE‐1 was obtained, which displayed high rates of cleavage with strong specificity. A fluorescent biosensor was designed for the detection of P. aeruginosa in combination with the DNAzyme. A detection limit as low as 1.2 cfu/ml was observed. Using proteases and filtration, it was determined that the target was a protein with a molecular weight of 10 kDa–50 kDa. The DNAzyme was combined with a polystyrene board to construct a simple indicator plate sensor which produced a color that identified the target within 10 min. The results were reliable when tap water and food samples were tested. The present study provides a novel experimental strategy for the development of sensors based on a DNAzyme to rapidly detect P. aeruginosa in the field.

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Exogenous acquisition of Pseudomonas aeruginosa in intensive care units: a prospective multi-centre study (DYNAPYO study)

Cited by 14 publications

References 38 publications

Impact of lockdown on the microbiological status of the hospital water network during COVID-19 pandemic

Impact of lockdown on the microbiological status of the hospital water network during COVID-19 pandemic

Time Series Genomics of Pseudomonas aeruginosa Reveals the Emergence of a Hypermutator Phenotype and Within-Host Evolution in Clinical Inpatients

Rapid detection of Pseudomonas aeruginosa using a DNAzyme‐based sensor

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