Exogenous Ascorbic Acid Mediated Abiotic Stress Tolerance in Plants

Xu, Yi; Huang, Bingru

doi:10.1007/978-3-319-74057-7_9

Cited by 7 publications

(8 citation statements)

References 89 publications

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“…AsA is one of the most abundant water‐soluble antioxidants in plants and acts as a key regulator in growth and development (Hossain et al, 2018 ), and abiotic (Billah et al, 2017 ; Farooq et al, 2013 ; Kobayakawa & Imai, 2017 ; Wang et al, 2017 ; Xu & Huang, 2018 ) as well as biotic stress tolerance (Boubakri, 2018 ; Egan et al, 2007 ; Fujiwara et al, 2013 ; Li et al, 2016 ). Oxidation of AsA is an important event in the stress‐induced AsA:DHA cycle, catalysed by the enzymes ascorbate oxidase (AO) and APX (Green & Fry, 2005 ; Stevens et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Dehydroascorbate induces plant resistance in rice against root‐knot nematode Meloidogyne graminicola

Chavan

Kesel

Desmedt

et al. 2022

Molecular Plant Pathology

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Ascorbic acid (AsA) is an important antioxidant in plants and regulates various physiological processes. In this study, we show that exogenous treatments with the oxidized form of AsA, that is, dehydroascorbate (DHA), activates induced systemic resistance in rice against the root‐knot nematode Meloidogyne graminicola , and investigate the molecular and biochemical mechanisms underlying this phenotype. Detailed transcriptome analysis on roots of rice plants showed an early and robust transcriptional response on foliar DHA treatment, with induction of several genes related to plant stress responses, immunity, antioxidant activity, and secondary metabolism already at 1 day after treatment. Quantitative and qualitative evaluation of H 2 O 2 levels confirmed the appearance of a reactive oxygen species (ROS) burst on DHA treatment, both at the site of treatment and systemically. Experiments using chemical ROS inhibitors or scavengers confirmed that H 2 O 2 accumulation contributes to DHA‐based induced resistance. Furthermore, hormone measurements in DHA‐treated plants showed a significant systemic accumulation of the defence hormone salicylic acid (SA). The role of the SA pathway in DHA‐based induced resistance was confirmed by nematode infection experiments using an SA‐signalling deficient WRKY45 ‐RNAi line and reverse transcription‐quantitative PCR on SA marker genes. Our results collectively reveal that DHA activates induced systemic resistance in rice against the root‐knot nematode M. graminicola , mediated through the production of ROS and activation of the SA pathway.

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Section: Discussionmentioning

confidence: 99%

Dehydroascorbate induces plant resistance in rice against root‐knot nematode Meloidogyne graminicola

Chavan

Kesel

Desmedt

et al. 2022

Molecular Plant Pathology

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“…Ascorbate can be exogenously applied as a foliage application, seed treatment, and co-treatment for the alleviation of stress-induced damages [181]. Many researchers reported about the supplemental AsA-mediated antioxidant defense regulation in various plant species under different stressors, such as salt stress [182], drought [183], extreme temperature [184], ozone [185], and heavy metal stress [186].…”

Section: Exogenous Use Of Asa and Gsh In Conferring Abiotic Stressmentioning

confidence: 99%

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

et al. 2019

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Reactive oxygen species (ROS) generation is a usual phenomenon in a plant both under a normal and stressed condition. However, under unfavorable or adverse conditions, ROS production exceeds the capacity of the antioxidant defense system. Both non-enzymatic and enzymatic components of the antioxidant defense system either detoxify or scavenge ROS and mitigate their deleterious effects. The Ascorbate-Glutathione (AsA-GSH) pathway, also known as Asada–Halliwell pathway comprises of AsA, GSH, and four enzymes viz. ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase, play a vital role in detoxifying ROS. Apart from ROS detoxification, they also interact with other defense systems in plants and protect the plants from various abiotic stress-induced damages. Several plant studies revealed that the upregulation or overexpression of AsA-GSH pathway enzymes and the enhancement of the AsA and GSH levels conferred plants better tolerance to abiotic stresses by reducing the ROS. In this review, we summarize the recent progress of the research on AsA-GSH pathway in terms of oxidative stress tolerance in plants. We also focus on the defense mechanisms as well as molecular interactions.

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“…L-Ascorbic acid (Asc), the reduced form of vitamin C, is a major non-enzymic antioxidant that plays an important role in improving the fundamental functioning of plants under normal and abiotic stress conditions, including salinity [13]. Ascorbic acid is synthesized naturally in plant cells, and fully developed chloroplasts contain 30-40% of the total AsA.…”

Section: Introductionmentioning

confidence: 99%

“…Ascorbic acid is synthesized naturally in plant cells, and fully developed chloroplasts contain 30-40% of the total AsA. Photosynthesis pigment contents in stress-affected plants were enhanced by Asc treatment because Asc is associated with chloroplast [13]. The Asc is involved in a stress tolerance mechanism involving a H 2 O 2 scavenging cycle in the chloroplast and the AsA-GSH pathway, and it plays a critical role as a "redox buffer" in ROS metabolism [14,15].…”

Section: Introductionmentioning

confidence: 99%

Foliar Application of Ascorbic Acid and Tocopherol in Conferring Salt Tolerance in Rapeseed by Enhancing K+/Na+ Homeostasis, Osmoregulation, Antioxidant Defense, and Glyoxalase System

et al. 2023

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This study explored the role of exogenous α-Toc (0.5 mM) and Asc (1 mM) in alleviating the damaging effects of salt stress in rapeseed plants (Brassica campestris cv. BARI Sarisha-17). Exposure of 21-day-old plants to different levels of salt stress (75 mM and 150 mM NaCl) resulted in the higher accumulation of sodium ions (Na+), reduced potassium ion (K+) levels, lower K+/Na+ ratio, increased oxidative damage, chlorophyll (Chl) breakdown, and disrupted antioxidant and glyoxalase systems. Phenotype responses to salt stress included reductions in plant height, shoot fresh weight, dry weight, number of siliques plant−1, silique length, number of seeds silique−1, 1000-seed weight, and seed yield plant−1. Exogenous α-Toc and Asc applications enhanced the levels of endogenous ascorbate, glutathione (GSH), AsA/dehydroascorbate ratios, GSH/glutathione disulfide, ascorbate peroxidase, monodehydroascorbate reductase, glutathione reductase, glutathione peroxidase, and catalase activities in the salt-stressed plants. Exogenous α-Toc and Asc enhanced antioxidant defense system components and insured better oxidative stress tolerance, as indicated by reduced hydrogen peroxide generation, membrane lipid peroxidation, and electrolyte leakage. Exogenous α-Toc and Asc increased glyoxalase I and glyoxalase II activities in the salt-affected plants. Moreover, they regulated proline levels and increased the leaf relative water content, as well as the Chl level. Exogenous α-Toc and Asc also restored growth and improved yield attributes and seed yield per plants in the salt-affected rapeseed.

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Exogenous Ascorbic Acid Mediated Abiotic Stress Tolerance in Plants

Cited by 7 publications

References 89 publications

Dehydroascorbate induces plant resistance in rice against root‐knot nematode Meloidogyne graminicola

Dehydroascorbate induces plant resistance in rice against root‐knot nematode Meloidogyne graminicola

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

Foliar Application of Ascorbic Acid and Tocopherol in Conferring Salt Tolerance in Rapeseed by Enhancing K+/Na+ Homeostasis, Osmoregulation, Antioxidant Defense, and Glyoxalase System

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