Exogenous ascorbic acid scarcely ameliorates inhibition of photosynthesis in rice leaves by O<sub>3</sub>

Kobayakawa, Hiroki; Imai, Katsu

doi:10.1080/1343943x.2016.1232149

Cited by 10 publications

(4 citation statements)

References 20 publications

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“…AsA is one of the most abundant water‐soluble antioxidants in plants and acts as a key regulator in growth and development (Hossain et al, 2018 ), and abiotic (Billah et al, 2017 ; Farooq et al, 2013 ; Kobayakawa & Imai, 2017 ; Wang et al, 2017 ; Xu & Huang, 2018 ) as well as biotic stress tolerance (Boubakri, 2018 ; Egan et al, 2007 ; Fujiwara et al, 2013 ; Li et al, 2016 ). Oxidation of AsA is an important event in the stress‐induced AsA:DHA cycle, catalysed by the enzymes ascorbate oxidase (AO) and APX (Green & Fry, 2005 ; Stevens et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Dehydroascorbate induces plant resistance in rice against root‐knot nematode Meloidogyne graminicola

Chavan

Kesel

Desmedt

et al. 2022

Molecular Plant Pathology

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Ascorbic acid (AsA) is an important antioxidant in plants and regulates various physiological processes. In this study, we show that exogenous treatments with the oxidized form of AsA, that is, dehydroascorbate (DHA), activates induced systemic resistance in rice against the root‐knot nematode Meloidogyne graminicola , and investigate the molecular and biochemical mechanisms underlying this phenotype. Detailed transcriptome analysis on roots of rice plants showed an early and robust transcriptional response on foliar DHA treatment, with induction of several genes related to plant stress responses, immunity, antioxidant activity, and secondary metabolism already at 1 day after treatment. Quantitative and qualitative evaluation of H 2 O 2 levels confirmed the appearance of a reactive oxygen species (ROS) burst on DHA treatment, both at the site of treatment and systemically. Experiments using chemical ROS inhibitors or scavengers confirmed that H 2 O 2 accumulation contributes to DHA‐based induced resistance. Furthermore, hormone measurements in DHA‐treated plants showed a significant systemic accumulation of the defence hormone salicylic acid (SA). The role of the SA pathway in DHA‐based induced resistance was confirmed by nematode infection experiments using an SA‐signalling deficient WRKY45 ‐RNAi line and reverse transcription‐quantitative PCR on SA marker genes. Our results collectively reveal that DHA activates induced systemic resistance in rice against the root‐knot nematode M. graminicola , mediated through the production of ROS and activation of the SA pathway.

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Section: Discussionmentioning

confidence: 99%

Dehydroascorbate induces plant resistance in rice against root‐knot nematode Meloidogyne graminicola

Chavan

Kesel

Desmedt

et al. 2022

Molecular Plant Pathology

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“…Ascorbate can be exogenously applied as a foliage application, seed treatment, and co-treatment for the alleviation of stress-induced damages [181]. Many researchers reported about the supplemental AsA-mediated antioxidant defense regulation in various plant species under different stressors, such as salt stress [182], drought [183], extreme temperature [184], ozone [185], and heavy metal stress [186].…”

Section: Exogenous Use Of Asa and Gsh In Conferring Abiotic Stressmentioning

confidence: 99%

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

et al. 2019

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Reactive oxygen species (ROS) generation is a usual phenomenon in a plant both under a normal and stressed condition. However, under unfavorable or adverse conditions, ROS production exceeds the capacity of the antioxidant defense system. Both non-enzymatic and enzymatic components of the antioxidant defense system either detoxify or scavenge ROS and mitigate their deleterious effects. The Ascorbate-Glutathione (AsA-GSH) pathway, also known as Asada–Halliwell pathway comprises of AsA, GSH, and four enzymes viz. ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase, play a vital role in detoxifying ROS. Apart from ROS detoxification, they also interact with other defense systems in plants and protect the plants from various abiotic stress-induced damages. Several plant studies revealed that the upregulation or overexpression of AsA-GSH pathway enzymes and the enhancement of the AsA and GSH levels conferred plants better tolerance to abiotic stresses by reducing the ROS. In this review, we summarize the recent progress of the research on AsA-GSH pathway in terms of oxidative stress tolerance in plants. We also focus on the defense mechanisms as well as molecular interactions.

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“…AsA can be absorbed into plants and increase endogenous AsA content. Exogenous application of AsA plays an important role in many plants exposed to different types of abiotic stresses, including salinity [13], drought [14], heat [15], osmotic [16], and ozone [17]. AsA has been used to ameliorate abiotic stresses, and studies that improve plant growth under salt stress are the most common.…”

Section: Introductionmentioning

confidence: 99%

Effects of exogenous ascorbic acid on seed germination and seedling salt-tolerance of alfalfa

2021

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Alfalfa (Medicago sativa L.) is an important legume crop for forage, agriculture, and environment in the world. Ascorbic acid (AsA) plays positive roles in plants. However, its effects on germination and salt-tolerance of alfalfa are unknown. The effects of AsA applications on seed germination and seedling salt-tolerance of alfalfa were investigated. The results revealed that 0.1 and 1 mmol L-1 of exogenous AsA increased germination, amylase, and protease, as well as seedling length, fresh weight (FW), dry weight (DW), and endogenous AsA both in the shoots and roots, except that 1 mmol L-1 AsA reduced the activities of α-amylase, β-amylase and protease on day 3. However, 10 and 100 mmol L-1 AsA inhibited these parameters and even caused serious rot. It indicates that 0.1 mmol L-1 AsA has the optimal effects, whereas 100 mmol L-1 AsA has the worst impacts. Another part of the results showed that 0.1 mmol L-1 AsA not only enhanced stem elongation, FW and DW, but also increased chlorophyll and carotenoids both under non-stress and 150 mmol L-1 NaCl stress. Furthermore, 0.1 mmol L-1 AsA mitigated the damages of membrane permeability, malondialdehyde, and excessive reactive oxygen species (ROS) and ions both in the shoots and roots under 150 mmol L-1 NaCl stress. Hence, 0.1 mmol L-1 AsA improves growth and induces salt-tolerance by inhibiting excessive ROS, down-regulating the ion toxicity and up-regulating the antioxidant system. The principal component analysis included two main components both in the shoots and roots, and it explained the results well. In summary, the optimum concentration of 0.1 mmol L-1 AsA can be implemented to improve the seed germination and seedling growth of alfalfa under salt stress.

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Exogenous ascorbic acid scarcely ameliorates inhibition of photosynthesis in rice leaves by O₃

Cited by 10 publications

References 20 publications

Dehydroascorbate induces plant resistance in rice against root‐knot nematode Meloidogyne graminicola

Dehydroascorbate induces plant resistance in rice against root‐knot nematode Meloidogyne graminicola

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

Effects of exogenous ascorbic acid on seed germination and seedling salt-tolerance of alfalfa

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Exogenous ascorbic acid scarcely ameliorates inhibition of photosynthesis in rice leaves by O3

Cited by 10 publications

References 20 publications

Dehydroascorbate induces plant resistance in rice against root‐knot nematode Meloidogyne graminicola

Dehydroascorbate induces plant resistance in rice against root‐knot nematode Meloidogyne graminicola

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

Effects of exogenous ascorbic acid on seed germination and seedling salt-tolerance of alfalfa

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Exogenous ascorbic acid scarcely ameliorates inhibition of photosynthesis in rice leaves by O₃