Exogenous calcium affects nitrogen metabolism in root-zone hypoxia-stressed muskmelon roots and enhances short-term hypoxia tolerance

Gao, Hongbo; Jia, Yongxia; Guo, Shirong; Lv, Guiyun; Wang, Tian; Li, Juan

doi:10.1016/j.jplph.2011.01.022

Cited by 70 publications

(41 citation statements)

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“…Therefore, it is not surprising that calcium is implicated in plant metabolism regulation signaling, particularly in association with oxygen deprivation [64]. According to Gao et al, exogenous calcium induces the promotion of physiologically active factors and matters in muskmelon plants, as compared to the factors and matters observed in plants under hypoxic stress after 6 days [65]. CaCl 2 pretreatment increased the accumulation of amino acids in rice roots under anaerobic stress, possibly via a Ca-Camodulin complex involved in the transduction of an anaerobic signal that inhibits proteolysis and solute release [9].…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots

Xin-shi

Tian

et al. 2012

Proteome Sci

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BackgroundHypoxia acts as a plant stress factor, particularly in cucumbers plants under hydroponic culture. Calcium is involved in stress signal transmission and in the growth of plants. To determine the effect of exogenous calcium on hypoxic-responsive proteins in cucumber (Cucumis sativus L. cv. Jinchun No.2) roots, proteomic analysis was performed using two-dimensional electrophoresis (2-DE) and mass spectrometry.ResultsCucumber roots were used to analyze the influence of hypoxia on plants. The expressions of 38 protein spots corresponding to enzymes were shown to change in response to hypoxia. Of these, 30 spots were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS analysis). The proteins were categorized according to functional groups, including glycolysis, the tricarboxylic acid (TCA) cycle, fermentative metabolism, nitrogen metabolism, energy metabolism, protein synthesis and defense against stress. Exogenous calcium appeared to alleviate hypoxic stress via these metabolic and physiological systems. Western blotting was used to analyze the accumulation of alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC); calcium further increased the expression of ADH and PDC under hypoxia. In addition, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to assess the transcript levels of differentially expressed proteins.ConclusionsExogenous calcium enhanced the expression of enzymes involved in glycolysis, the TCA cycle, fermentative metabolism, nitrogen metabolism, and reactive oxygen species (ROS) defense in plants under hypoxia. Calcium appears to induce hypoxic tolerance of cucumber seedlings. These phenomena have prompted us to further investigate the mechanisms by which cucumbers respond to exogenous calcium under hypoxia.

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Section: Resultsmentioning

confidence: 99%

Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots

Xin-shi

Tian

et al. 2012

Proteome Sci

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“…Ca 2+ is a ubiquitous secondary messenger and is involved in responses to myriad developmental, hormonal, and environmental cues in plant cells, including the hypoxia response (White and Broadley, 2003;Gao et al, 2011;Oh et al, 2014). Initial evidence for a role of calcium in hypoxia responses came from work with maize (Zea mays) seedlings, where pretreatment with Ruthenium Red (RR; an inhibitor of organellar Ca 2+ release) followed by exposure to low-oxygen stress inhibited increases in ALCOHOL DEHYDROGENASE1 (ADH1) transcript and impaired poststress survival of the seedlings (Subbaiah et al, 1994a(Subbaiah et al, , 1994b.…”

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confidence: 99%

Arabidopsis CML38, a Calcium Sensor That Localizes to Ribonucleoprotein Complexes under Hypoxia Stress

et al. 2015

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During waterlogging and the associated oxygen deprivation stress, plants respond by the induction of adaptive programs, including the redirected expression of gene networks toward the synthesis of core hypoxia-response proteins. Among these core response proteins in Arabidopsis (Arabidopsis thaliana) is the calcium sensor CML38, a protein related to regulator of gene silencing calmodulin-like proteins (rgsCaMs). CML38 transcripts are up-regulated more than 300-fold in roots within 6 h of hypoxia treatment. Transfer DNA insertional mutants of CML38 show an enhanced sensitivity to hypoxia stress, with lowered survival and more severe inhibition of root and shoot growth. By using yellow fluorescent protein (YFP) translational fusions, CML38 protein was found to be localized to cytosolic granule structures similar in morphology to hypoxia-induced stress granules. Immunoprecipitation of CML38 from the roots of hypoxia-challenged transgenic plants harboring CML38pro::CML38: YFP followed by liquid chromatography-tandem mass spectrometry analysis revealed the presence of protein targets associated with messenger RNA ribonucleoprotein (mRNP) complexes including stress granules, which are known to accumulate as messenger RNA storage and triage centers during hypoxia. This finding is further supported by the colocalization of CML38 with the mRNP stress granule marker RNA Binding Protein 47 (RBP47) upon cotransfection of Nicotiana benthamiana leaves. Ruthenium Red treatment results in the loss of CML38 signal in cytosolic granules, suggesting that calcium is necessary for stress granule association. These results confirm that CML38 is a core hypoxia response calcium sensor protein and suggest that it serves as a potential calcium signaling target within stress granules and other mRNPs that accumulate during flooding stress responses.

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“…The effects of applied exogenous calcium in our study are similar to those described in muskmelon (Cucumis melo L. var. reticulates Naud) under hypoxia-stressed conditions (Gao et al, 2011). Moreover, exogenous calcium treatment significantly enhanced the IDH activity at 0°C.…”

Section: Discussionmentioning

confidence: 87%

Calcium Alleviates Temperature Stress by Regulating Nitrogen and Respiratory Metabolism in Malus baccata Roots

Hong¹,

Li²,

Ma³

et al. 2016

IJAB

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The effects of calcium on respiratory and nitrogen metabolism of apple roots (Malus baccata Borkh.) exposed to temperature stress (5°C ~ 20°C ~ 0°C) were investigated. Seedlings were treated with distilled water (control), calcium chloride (CaCl2) or calmodulin antagonist trifluoperazine (TFP) before temperature stress. Temperature was increased from 5°C to 20°C (1°C h ). Temperature stress decreased root vitality and increased root malondialdehyde (MDA) concentration, the effect of which was exacerbated by TFP treatment. Treatment with CaCl2 improved root vitality and decreased root MDA concentration. At 20°C, exogenous CaCl2 alleviated the negative effects of temperature stress on the total respiration rate by enhancing the activity of tricarboxylic acid cycle (TCA). Activities of key enzyme in nitrogen metabolism were strongly inhibited by temperature stress. Exogenous CaCl2 significantly increased key enzyme activities of nitrogen metabolism compared to the control. However, the TFP treatment markedly reduced the activity of glutamate synthase (GOGAT) at 20°C and noticeably inhibited glutamate dehydrogenase (GDH) activity during the entire temperature stress period. The data showed that the Ca 2+ -calmodulin (Ca 2+ -CaM) signal system was involved in increase of GOGAT and GDH activity that occurred with an increase in temperature, and played a role in the increase in the total respiration rate and GDH activity which occurred with a decrease in temperature. Cultural practices that improve plant calcium (Ca) status in the early spring may mitigate damage induced by temperature stress.

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Exogenous calcium affects nitrogen metabolism in root-zone hypoxia-stressed muskmelon roots and enhances short-term hypoxia tolerance

Cited by 70 publications

References 42 publications

Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots

Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots

Arabidopsis CML38, a Calcium Sensor That Localizes to Ribonucleoprotein Complexes under Hypoxia Stress

Calcium Alleviates Temperature Stress by Regulating Nitrogen and Respiratory Metabolism in Malus baccata Roots

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