Exogenous hydrogen sulfide attenuates cerebral ischemia–reperfusion injury by inhibiting autophagy in mice

Abstract

Hydrogen Sulfide, the third gas transmitter, has been proven to be neuroprotective in cerebral ischemia injury, but whether its effect is mediated by regulating autophagy is not yet clear. The present study was undertaken to explore the underlying mechanisms of exogenous H 2 S on autophagy regulation in cerebral ischemia. The effects and its connection with autophagy of NaHS, a H 2 S donor, were observed through neurological deficits and cerebral infarct volume in middle cerebral artery occlusion (MCAO) mice; autophagy related proteins and autophagy complex levels in ischemia hemisphere were detected with western blot assay. Compared to model group, NaHS significantly decreased infarct volume and improved neurological deficits; Rapamycin, an autophagy activator, abolished the effect of NaHS; NaHS decreased the expression of LC3-II and up-regulated p62 expression in ischemia cortex 24 hours after ischemia. However, NaHS did not significantly influence Beclin-1 expression. H 2 S has neuroprotective effect on ischemia injury in MCAO mice, this effect is associated with its influence in down-regulating autophagosomes accumulation.

Sodium hydrosulfide attenuates cerebral ischemia/reperfusion injury by suppressing overactivated autophagy in rats

“…During the preparation of this article, Shui et al . reported that H 2 S attenuates cerebral ischemia/reperfusion injury by inhibiting autophagy in mice. In combination with that work, the present study provides solid evidence that H 2 S is a protective factor against cerebral ischemia/reperfusion injury.…”

“…Accumulated evidence indicates that H 2 S exerts a protective effect against ischemia/reperfusion injury, such as myocardial ischemia/reperfusion injury and kidney ischemia/reperfusion injury . Recently, it has been reported that H 2 S attenuates cerebral ischemia/reperfusion injury in a mouse model . Nonetheless, the exact mechanisms and identities of molecules responsible for the neuroprotective effect of H 2 S against cerebral ischemia/reperfusion injury remain incompletely defined.…”

Hydrogen sulfide alleviates myocardial fibrosis in mice with alcoholic cardiomyopathy by downregulating autophagy

“…Yet, H 2 S was also reported by previous studies to participate in the regulation of cell autophagy ( 42 ). In addition, H 2 S has been identified to remit myocardial ischemia reperfusion injury ( 43 ). While in the study performed by Zhou et al , H 2 S improved the cardiac function of smoking rats by downregulating cell autophagy in the myocardium ( 15 ).…”

Hydrogen Sulfide Plays an Important Protective Role by Influencing Autophagy in Diseases

“…In addition, H 2 S did not affect the expression of Beclin1. Since the level of Beclin 1 did not accurately reflect the activity of Beclin-1-VPS34-AMBRA1, the role of Beclin-1 complex in regulating cell autophagy by H 2 S needed further study (Shui et al 2016, Klionsky et al 2008. Ischemic heart disease is especially important in the world with high mortality.…”