Exogenous Hydrogen Sulfide Within the Nucleus Ambiguus Inhibits Gastrointestinal Motility in Rats

Sun, Haitao; Ding, Haikun; Shi, Yuan; Li, Chenyu; Jin, Haoran; Yang, Xiaoyue; Chen, Zhaosong; Tian, Pengpeng; Zhu, Jianping; Sun, Hongchen

doi:10.3389/fphys.2020.545184

Cited by 3 publications

(5 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Capsazepine was also found to inhibit NaHS-induced pyloric smooth muscle relaxation, indicating that the effect of H 2 S donors on enhancing gastric emptying and inducing pyloric sphincter relaxation is mediated by the activation of TRPV1 receptors. Although there are many mechanisms by which this effect can be manifested, our data support the theory of smooth muscle relaxation induced by afferent neuronal TRPV1 receptor activation[ 45 , 60 ].…”

Section: Discussionsupporting

confidence: 85%

“…Our experiments revealed that the injection of the NF-κB pathway blocker PDTC followed by NaHS eliminated the modulation of gastric function by NaHS. H 2 S is mainly synthesized in the brain by the CBS enzyme, andwe found that NaHS injection in both the ambiguous and paraventricular nuclei inhibited gastric motility in rats via the NF-κB pathway[ 44 , 45 ], that the injection of NaHS to the brain blocked inflammation-associated apoptosis, and that treatment with NaHS reduced the expression of inflammatory factors in astrocytes and microglia due to Alzheimer’s disease[ 46 ]. H 2 S reduces the levels of phosphorylated p38 MAPK and phosphorylated p65 NF-κB in vivo .…”

Section: Discussionmentioning

confidence: 99%

“…Capsaicin also slows down gastric motility through TRPV1-induced excitation of gastric sensory nerve fibers[ 62 ]. A possible role of capsaicin-sensitive vagal afferent nerves in gastric mucosal injury and prevention has been demonstrated and is associated with the release of SP[ 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…We evaluated the following subgroups to investigate the regulatory effects of NaHS in the NTS on gastric function and its underlying mechanisms. The chosen doses were based on pre-experiments and relevant literature[ 31 ]: (1) The effect of microinjection of NaHS (0.1 µL, 4 nmol; n = 6) in NTS on gastric motility; (2) the effect of microinjection of NaHS (0.1 µL, 8 nmol; n = 6) in NTS on gastric motility; (3) the effect of microinjection of saline (0.1 µL; n = 6) in NTS on gastric motility as a control group; (4) the effect of microinjection of NaHS (0.1 µL, 4 nmol) + PDTC (0.1 µL; n = 6) in NTS on gastric motility; (5) microinjection of NaHS (0.1 µL, 4 nmol) + Capsazepine (0.1 µL, 4 nmol; n = 6) in NTS on gastric motility; and (6) microinjection of NaHS (0.1 µL, 4 nmol) + L703606 (0.1 µL, 4 nmol; n = 6) in NTS on gastric motility.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Effect of exogenous hydrogen sulfide in the nucleus tractus solitarius on gastric motility in rats

Sun¹,

Li²,

Shi³

et al. 2023

World J Gastroenterol

Self Cite

View full text Add to dashboard Cite

Tumor necrosis factor-α (TNF-α) antagonists, the first biologics approved for treating patients with inflammatory bowel disease (IBD), are effective for the induction and maintenance of remission and significantly improving prognosis. However, up to one-third of treated patients show primary nonresponse (PNR) to anti-TNF-α therapies, and 23%-50% of IBD patients experience loss of response (LOR) to these biologics during subsequent treatment. There is still no recognized predictor for evaluating the efficacy of anti-TNF drugs. This review summarizes the existing predictors of PNR and LOR to anti-TNF in IBD patients. Most predictors remain controversial, and only previous surgical history, disease manifestations, drug concentrations, antidrug antibodies, serum albumin, some biologic markers, and some genetic markers may be potentially predictive. In addition, we also discuss the next steps of treatment for patients with PNR or LOR to TNF antagonists. Therapeutic drug monitoring plays an important role in treatment selection. Dose escalation, combination therapy, switching to a different anti-TNF drug, or switching to a biologic with a different mechanism of action can be selected based on the concentration of the drug and/or antidrug antibodies.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of exogenous hydrogen sulfide in the nucleus tractus solitarius on gastric motility in rats

Sun¹,

Li²,

Shi³

et al. 2023

World J Gastroenterol

Self Cite

View full text Add to dashboard Cite

show abstract

“…It also acts in a dose-dependent manner in appropriate physiological concentrations (10-130 μM) Moshal et al (2008). We also observed inhibition of gastric motility in the rats after exogenous H 2 S donor NaHS was injected in the NA Sun et al (2020). Therefore, we suggest that H 2 S can modulate gastric function through the NMDA receptors, but within the normal physiological concentration range.…”

Section: Discussionmentioning

confidence: 54%

Investigating the L-Glu-NMDA receptor-H2S-NMDA receptor pathway that regulates gastric function in rats’ nucleus ambiguus

Sun,

Li,

Shi

et al. 2024

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

BackgroundIn previous investigations, we explored the regulation of gastric function by hydrogen sulfide (H2S) and L-glutamate (L-Glu) injections in the nucleus ambiguus (NA). We also determined that both H2S and L-Glu have roles to play in the physiological activities of the body, and that NA is an important nucleus for receiving visceral sensations. The purpose of this study was to explore the potential pathway link between L-Glu and H2S, resulting in the regulation of gastric function.MethodsPhysiological saline (PS), L-glutamate (L-Glu, 2 nmol), NaHS (2 nmol), D-2-amino-5-phopho-novalerate (D-AP5, 2 nmol) + L-Glu (2 nmol), aminooxyacetic acid (AOAA, 2 nmol) + L-Glu (2 nmol), D-AP5 (2 nmol) + NaHS (2 nmol) were injected into the NA. A balloon was inserted into the stomach to observe gastric pressure and for recording the changes of gastric smooth muscle contraction curve. The gastric fluid was collected by esophageal perfusion and for recording the change of gastric pH value.ResultsInjecting L-Glu in NA was found to significantly inhibit gastric motility and promote gastric acid secretion in rats (p < 0.01). On the other hand, injecting the PS, pre-injection N-methyl-D-aspartate (NMDA) receptor blocker D-AP5, cystathionine beta-synthase (CBS) inhibitor AOAA and re-injection L-Glu did not result in significant changes (p > 0.05). The same injection NaHS significantly inhibit gastric motility and promote gastric acid secretion in rats (p < 0.01), but is eliminated by injection D-AP5 (p > 0.05).ConclusionThe results indicate that both exogenous L-Glu and H2S injected in NA regulate gastric motility and gastric acid secretion through NMDA receptors. This suggests that NA has an L-Glu-NMDA receptor-CBS-H2S pathway that regulates gastric function.

show abstract

Physiological roles of hydrogen sulfide in mammalian cells, tissues, and organs

Cirino

Szabó

Papapetropoulos

2023

Physiological Reviews

213

142

View full text Add to dashboard Cite

H2S belongs to the class of molecules known as gasotransmitters, which also includes nitric oxide (NO) and carbon monoxide (CO). Three enzymes are recognized as endogenous sources of H2S in various cells and tissues: cystathionine g-lyase (CSE), cystathionine β-synthase (CBS) and 3-mercaptopyruvate sulfurtransferase (3-MST). The current article reviews the regulation of these enzymes as well as the pathways of their enzymatic and non-enzymatic degradation and elimination. The multiple interactions of H2S with other labile endogenous molecules (e.g. NO) and reactive oxygen species are also outlined. The various biological targets and signaling pathways are discussed, with special reference to H2S and oxidative posttranscriptional modification of proteins, the effect of H2S on channels and intracellular second messenger pathways, the regulation of gene transcription and translation and the regulation of cellular bioenergetics and metabolism. The pharmacological and molecular tools currently available to study H2S physiology are also reviewed, including their utility and limitations. In subsequent sections, the role of H2S in the regulation of various physiological and cellular functions is reviewed. The physiological role of H2S in various cell types and organ systems are overviewed. Finally, the role of H2S in the regulation of various organ functions is discussed as well as the characteristic bell-shaped biphasic effects of H2S. In addition, key pathophysiological aspects, debated areas, and future research and translational areas are identified A wide array of significant roles of H2S in the physiological regulation of all organ functions emerges from this review.

show abstract

Exogenous Hydrogen Sulfide Within the Nucleus Ambiguus Inhibits Gastrointestinal Motility in Rats

Cited by 3 publications

References 52 publications

Effect of exogenous hydrogen sulfide in the nucleus tractus solitarius on gastric motility in rats

Effect of exogenous hydrogen sulfide in the nucleus tractus solitarius on gastric motility in rats

Investigating the L-Glu-NMDA receptor-H2S-NMDA receptor pathway that regulates gastric function in rats’ nucleus ambiguus

Physiological roles of hydrogen sulfide in mammalian cells, tissues, and organs

Contact Info

Product

Resources

About