Exogenous jasmonic acid enhances oxidative protection of Lemna valdiviana subjected to arsenic

Abstract

Oxidative damage is one of the most harmful effects arising from arsenic (As) toxicity in plants. Herein, the role of exogenous jasmonic acid (JA) in the modulation of As-induced oxidative stress in Lemna valdiviana was investigated. Plants were grown for 24 h in Clark's nutritive solution containing As (4.0 mg L −1) or As + JA (50, 100, 250 and 500 µM). Chlorophyll a and b content decreased under As stress, either in isolation or associated with JA. The decreased chlorophyll a/b ratio in As-exposed plants was recovered by JA treatment at 100 µM. The carotenoid content was higher in plants exposed to As compared to controls and lower when it was associated with JA. Arsenic triggered the accumulation of O 2 •− and H 2 O 2 , in addition to severely increasing lipid peroxidation. Application of JA in As-grown plants resulted in lower O 2 •− content and lipid peroxidation than in those grown under As alone, as a result of enhanced SOD activity. On the other hand, H 2 O 2 accumulation was increased by JA in As-stressed plants, associated with higher CAT, POX and GPX activity. The As content and bioaccumulation factor (BF) were improved by application of JA in the nutritive solution at 250 and 500 µM. Our findings indicate that JA modulates the pigment balance, thereby fine-tuning energy dissipation as well as alleviating As-induced oxidative damage in L. valdiviana through modulation of ROS homeostasis and improvement of the antioxidant enzymatic system, allowing increased accumulation of As without showing major damage.

Unveiling Innovative Approaches to Mitigate Metals/Metalloids Toxicity for Sustainable Agriculture

“…Another study reported that applying JA at 100 μM restored the Chl a / b ratio in plants exposed to As. JA also enhanced H 2 O 2 accumulation in As‐stressed plants, accompanied by increased CAT, POX, and GPX activity (Coelho et al, 2020). Karimi and Ghasempour (2019) found that the combined application of SA and JA alleviates the Ni‐toxicity by strengthening the antioxidant defense mechanism and reducing H 2 O 2 concentration in the shoots of metallicolous and non‐metallicolous Alyssum inflatum Náyr.…”

Arsenic perception and signaling: The yet unexplored world

“…Hormone distribution along roots could be a critical factor in the integration of arsenic perception with root architecture. Different hormones, like jasmonic acid, salicylic acid, brassinosteroids, ethylene, gibberellins, and strigolactones have been involved in arsenic tolerance, although their effect is mostly due to their capability to control oxidative stress (Surgun-Acar and Zemheri-Navruz, 2019;Coelho et al, 2020;Kaya et al, 2020;Mostofa et al, 2021a,b;Samanta et al, 2021;Singh et al, 2021). However, several experiments point out that ABA, auxin and cytokinins have a specific role in root growth adaptation to arsenic toxicity besides oxidative stress mitigation.…”

Role of Jasmonic and Salicylic Acid on Enzymatic Changes in the Root of Two Alyssum inflatum Náyr. Populations Exposed to Nickel Toxicity

“…Jasmonates [JA and methyl jasmonate (MeJA)] are considered endogenous-growth substances that play a signi cant role by modulating signal transduction mechanisms under stress conditions in numerous plant species (Raza et al 2020). Many studies have been shown that JA and MeJA supplementation can recover the toxic effects of heavy metals in plants that have been described in diverse plants such as like soybean (Glycine max) (Keramat et al 2009) and Mentha arvensis (Zaid and Mohammad 2018) under high cadmium (Cd) concentration, and Lemna valdiviana under arsenic (As) toxicity (Coelho et al 2020).…”

“…Mir et al (2018) also reported that JA's supplement has an ameliorating effect on Ni toxicity in G. max plants exposed to NiCl 2 (4-mM) by improving SOD and CAT activities. So far, there have been several reports on the protective role of JA against metal stress in plants, including G. max under Ni toxicity (Sirhindi et al 2015), Lycopersicon esculentum (Bali et al 2019b), and Arabidopsis thaliana (Lei et al 2020) under Cd stress, Puccinellia tenui ora under B-toxicity toxicity (Zhao et al 2019), tomato under Pb toxicity (Bali et al 2019a), and L. valdiviana exposed to As stress(Coelho et al 2020). It is found that JA as a signaling molecule can increase genes expression involved in encoding antioxidative enzymes in plant cells for decreasing heavy metals toxicity by reducing ROS contents.…”