Exogenous nitric oxide inhibits the respiratory metabolism of postharvest wax apple fruit and its role in the delayed cottony softening

Zhang, Yixiang; Wang, Ying; Fa-he, Chen; He, Fazhi; Wu, Guorong; Shi, Linfan; Lin, Hetong

doi:10.1016/j.scienta.2023.112043

Cited by 14 publications

(1 citation statement)

References 45 publications

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“…CS, IDH, α-KGDH, and SDH are key enzymes in the TCA cycle [ 16 ]. The first crucial step in the TCA cycle is catalyzed by CS, which facilitates the irreversible conversion of acetyl-CoA oxaloacetate to citric acid [ 41 ]. Another pivotal enzyme in the TCA cycle is IDH, which acts as a rate-limiting enzyme and catalyzes the decarboxylation and dehydrogenation of isocitric acid to produce α-ketoglutaric acid [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Regulation of Embden–Meyerhof–Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (Carica papaya L.)

Huang,

Tao,

Zhu

et al. 2023

IJMS

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Postharvest abnormal chilling injury (CI) behavior in papaya (Carica papaya L.) fruit is a rare phenomenon that may be associated with respiratory metabolism. This study thus aimed to investigate the impacts of storage temperatures (1 and 6 °C) on the respiratory metabolism of postharvest papaya and its impact on CI development. Results demonstrated that 1 °C storage reduced the activities of hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), citrate synthase (CS), and α-ketoglutarate dehydrogenase (α-KGDH) and regulated the expression of corresponding enzymes in the Embden–Meyerhof–Parnas (EMP) pathway and tricarboxylic acid (TCA) cycle compared with 6 °C storage, resulting in a lower respiration rate of the EMP-TCA pathway and mitigating the development of CI. Meanwhile, lower contents of nicotinamide adenine dinucleotide (hydrogen) (NAD(H)) were observed in papaya fruit stored at 1 °C. Notably, papaya fruit stored at 1 °C maintained higher activity and transcriptional levels of SDH and IDH during the whole storage period. These findings suggest that 1 °C storage reduced the respiration rate of the EMP-TCA pathway by reducing the expression level and activity of related enzymes, which is conducive to the reduction of respiration substrate consumption and finally alleviating the occurrence of CI.

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Section: Discussionmentioning

confidence: 99%

Regulation of Embden–Meyerhof–Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (Carica papaya L.)

Huang,

Tao,

Zhu

et al. 2023

IJMS

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Quantitative analyses of major enzyme activities in postharvest fruit

Li,

Zheng,

et al. 2024

Future Postharvest and Food

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The quantitative determination of major enzyme activities in postharvest fruit is a necessary biochemical experiment to investigate the physiological and metabolic mechanisms of fruit ripeness, senescence, and stress responses. However, it is necessary to choose appropriate methods to stably extract and determine target analytes from complex plant matrices due to the dynamic changes in enzyme activities of postharvest fruit during storage. Here, we outline sensitive and accurate methods for measuring fruit enzyme activities associated with key physiological processes including respiration, reactive oxygen species scavenging, membrane lipid degradation, energy synthesis, cell walls degradation, and disease resistance. This manuscript aims to provide guidance for future research in postharvest biology.

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Exogenous nitric oxide treatment delays the senescence of postharvest mung bean sprouts by regulating ascorbic acid metabolism

Wang,

Qiu,

Zhang

et al. 2024

J Sci Food Agric

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BACKGROUNDThis study evaluated the effects of nitric oxide (NO) treatment on ascorbic acid (AsA) metabolism and mung bean sprout quality. It examined changes in the AsA content, enzyme activity associated with AsA metabolism, antioxidant capacity, cell membrane composition, and cellular structure to clarify the effects of NO on mung bean sprouts.RESULTSNitric oxide treatment preserved mung bean sprout quality by enhancing significantly the activity of enzymes involved in the l‐galactose pathway (including guanosine diphosphate (GDP)glutathione (‐d‐mannose pyrophosphorylase, GDP‐mannose‐3′,5′‐epimerase, GDP‐l‐galactose phosphorylase, l‐galactose‐1‐phosphate phosphatase, l‐galactose dehydrogenase, and l‐galactose‐1,4‐lactone dehydrogenase) and the AsA‐glutathione (GSH)(Beijing Solarbio Science and Technology Co.,Ltd., Beijing, China) cycle (including ascorbate peroxidase, ascorbic acid oxidase, glutathione reductase, dehydroascorbate reductase, and monodehydroascorbate reductase) during the germination and storage stage. Increased enzyme activity led to an increase in AsA content and enhanced antioxidant capacity, and reduced the membrane lipid damage in mung bean sprouts. This was demonstrated by higher levels of DPPH radical scavenging capacity, unsaturated fatty acids and phospholipids, along with lower levels of hydrogen peroxide, superoxide anions, and malondiadehyde, in NO‐treated mung bean sprouts. Scanning electron microscopy also revealed that NO treatment maintained the integrity of the cellular structure of the mung bean sprouts.CONCLUSIONNitric oxide accelerates AsA metabolism effectively by regulating the biosynthesis and regeneration of AsA in mung bean sprouts. These changes increased AsA levels, alleviated membrane lipid damage, delayed senescence, and maintained the quality of mung bean sprouts during storage. © 2024 Society of Chemical Industry.

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Exogenous nitric oxide inhibits the respiratory metabolism of postharvest wax apple fruit and its role in the delayed cottony softening

Cited by 14 publications

References 45 publications

Regulation of Embden–Meyerhof–Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (Carica papaya L.)

Regulation of Embden–Meyerhof–Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (Carica papaya L.)

Quantitative analyses of major enzyme activities in postharvest fruit

Exogenous nitric oxide treatment delays the senescence of postharvest mung bean sprouts by regulating ascorbic acid metabolism

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