Exogenous parathyroid hormone attenuates ovariectomy-induced skeletal muscle weakness in vivo

Fujimaki, Taro; Ando, Takashi; Hata, Takanori; Takayama, Yoshihiro; Ohba, Tetsuro; Ichikawa, Junichiro; Takiyama, Yoshihisa; Tatsuno, Rikito; Koyama, Katsuhiro; Haro, Hirotaka

doi:10.1016/j.bone.2021.116029

Cited by 9 publications

(8 citation statements)

References 74 publications

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“…Moreover, effective increases in the MHC protein assayed in the myotubes, obtained with the 30-min stimuli of PTH (1-84) and after 6 days of hSC myogenic differentiation, confirm the remarkable effect of PTH (1-84) on human myogenesis ( Figure 6 ). Our data for human muscle cells are in agreement with the PTH (1-34)-induced murine C 2 C 12 muscle cell differentiation, detected by Western blot [ 21 ].…”

Section: Discussionsupporting

confidence: 89%

“…The data are evidence that proliferation is not affected by a 30-min stimulation with the hormone at different concentrations ( Figure 4 ). Conversely, a previous study on the murine myoblast C 2 C 12 line treated for 72 h with several PTH (1-34) concentrations, ranging from 10 −8 to 10 −11 mol/L, highlighted that the hormone is able to induce C 2 C 12 proliferation after in vitro administration with all the treatments tested, leading us to assume that an extended length of treatments may influence the proliferation of hSCs, as reported in the C 2 C 12 cells [ 21 ].…”

Section: Discussionmentioning

confidence: 86%

“…Their data showed that monotherapy with PTH (1-34), at a dosage of 30 µg/kg body weight 3 times per week, significantly increased the proximal and distal femoral bone mineral density (BMD) and the percentage of the skeletal muscle mass in ovariectomized, tail-suspended rats [ 20 ]. Fujimaki et al investigated the effect of PTH (1-34) on skeletal muscle and dysfunction using a murine ovariectomized model; mice that received intraperitoneal injections of 80 µg/kg body weight 3 times a week for 20 weeks significantly ameliorated muscle weakness and increased the fiber cross-sectional area and satellite cells activity, but not the muscle weight [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Effects of PTH (1-84) on Human Skeletal Muscle-Derived Satellite Cells

Romagnoli

Zonefrati²,

Lucattelli

et al. 2023

Biomedicines

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Parathyroid hormone (PTH) is a hormone secreted by the parathyroid glands. Despite its well-known characterized anabolic and catabolic actions on the skeleton, the in vitro effects of PTH on skeletal muscle cells are limited and generally performed on animal models. The aim of this study was to evaluate the effects of a short impulse of PTH (1-84) on the proliferation and the differentiation of skeletal muscle satellite cells isolated from human biopsies. The cells were exposed for 30 min to different concentrations of PTH (1-84), from 10−6 mol/L to 10−12 mol/L. ELISA was used to assay cAMP and the myosin heavy-chain (MHC) protein. The proliferation was assayed by BrdU and the differentiation by RealTime-qPCR. A statistical analysis was performed by ANOVA followed by Bonferroni’s test. No significant variations in cAMP and the proliferation were detected in the isolated cells treated with PTH. On the other hand, 10−7 mol/L PTH on differentiated myotubes has shown significant increases in cAMP (p ≤ 0.05), in the expression of myogenic differentiation genes (p ≤ 0.001), and in the MHC protein (p ≤ 0.01) vs. untreated controls. This work demonstrates for the first time the in vitro effects of PTH (1-84) on human skeletal muscle cells and it opens new fields of investigation in muscle pathophysiology.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

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In Vitro Effects of PTH (1-84) on Human Skeletal Muscle-Derived Satellite Cells

Romagnoli

Zonefrati²,

Lucattelli

et al. 2023

Biomedicines

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“…A previous report showed that MCT1 and MCT4 protein levels decreased 20 wk after OVX (47). In the present study, although OVX decreased MCT4 protein content in the plantaris muscle, no effect of OVX on MCT1 protein content was detected.…”

Section: Discussionmentioning

confidence: 89%

Effects of Endurance Training on Metabolic Enzyme Activity and Transporter Proteins in Skeletal Muscle of Ovariectomized Mice

Takahashi

Kitaoka

Matsunaga

et al. 2022

Medicine &Amp; Science in Sports &Amp; Exercise

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PurposeEstrogen deficiency or insufficiency can occur under several conditions, leading to negative health outcomes. To establish an effective countermeasure against estrogen loss, we investigated the effects of endurance training on ovariectomy (OVX)-induced metabolic disturbances.MethodsFemale Institute of Cancer Research mice underwent OVX or sham operations. On day 7 of recovery, the mice were randomized to remain either sedentary or undergo 5 wk of treadmill running (15–20 m·min−1, 60 min, 5 d·wk−1). During week 5 of the training, all animals performed a treadmill running test (15 m·min−1, 60 min).ResultsAfter the experimental period, OVX resulted in greater gains in body mass, fat mass, and triglyceride content in the gastrocnemius muscle. OVX enhanced phosphofructokinase activity in the plantaris muscle and decreased lactate dehydrogenase activity in the plantaris and soleus muscles. OVX decreased the protein content of NDUFB8, a mitochondrial respiratory chain subunit, but did not decrease other mitochondrial proteins or enzyme activities. Endurance training significantly enhanced mitochondrial enzyme activity and protein content in the skeletal muscles. Although OVX increased the respiratory exchange ratio during the treadmill running test, and postexercise blood lactate levels, endurance training normalized these parameters.ConclusionsThe present findings suggest that endurance training is a viable strategy to counteract the negative metabolic consequences in hypoestrogenism.

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“…Parathyroid hormones affect blood calcium concentrations by regulating bone metabolism. Low doses of parathyroid hormone promote bone synthesis and lower blood calcium, while high doses of parathyroid hormone promote bone breakdown and elevate blood calcium 58 The presence of parathyroid hormones receptor in myocytes and the direct action of parathyroid hormone or its analogue, parathyroid hormone analogue, on muscles enhances muscle contraction and promotes muscle synthesis 59 . Parathyroid hormones also promote the synthesis of 1,25-dihydroxyvitamin D, thereby improving muscle strength 60 .…”

Section: Current Treatments For Sarcopeniamentioning

confidence: 99%

Potential application of anti-osteoporotic therapy to relieve sarcopenia in the elderly

Li,

Kong

et al. 2023

Annals of Medicine &Amp; Surgery

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Sarcopenia is a progressive and systemic skeletal muscle disorder associated with aging that- usually occurs with age in the elderly.Sarcopenia currently lacks effective pharmacological treatment modalities. Multiple pharmacological intervention modalities are available for osteoporosis, a comprehensive disease characterized by decreased systemic bone mass, degradation of bone microarchitecture, and increased bone fragility. Several recent studies have shown an extremely strong correlation between sarcopenia and osteoporosis, leading to the concept of “osteosarcopenia”. Therefore, it is possible to alleviate sarcopenia simultaneously by improving osteoporosis.

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Exogenous parathyroid hormone attenuates ovariectomy-induced skeletal muscle weakness in vivo

Cited by 9 publications

References 74 publications

In Vitro Effects of PTH (1-84) on Human Skeletal Muscle-Derived Satellite Cells

In Vitro Effects of PTH (1-84) on Human Skeletal Muscle-Derived Satellite Cells

Effects of Endurance Training on Metabolic Enzyme Activity and Transporter Proteins in Skeletal Muscle of Ovariectomized Mice

Potential application of anti-osteoporotic therapy to relieve sarcopenia in the elderly

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