Exogenous putrescine activates the arginine-polyamine pathway and inhibits the decomposition of endogenous polyamine in Anthurium andraeanum under chilling stress

Sun, Xiaolun; Yuan, Zebin; Wang, Bo; Zheng, Lulu; Tan, Jian

doi:10.1016/j.scienta.2021.110047

Cited by 11 publications

(5 citation statements)

References 28 publications

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“…The accumulation of Spd and Spm by D-Arg treatments also appears paradoxical because D-Arg should block Put synthesis, as a precursor of these two PAs. Cellular levels of PAs are known to be reduced after treatment with DMFA or other inhibitors (Minocha et al 1999), but in our system, PAs were promoted in response to D-Arg treatment, the same as in Araucaria angustifolia (Steiner et al 2007) and anthurium andraeanum (Sun et al 2021). The effects of the drugs usually used on PA content are also species dependent.…”

Section: Discussionmentioning

confidence: 54%

Influence of exogenous putrescine on somatic embryogenesis and regeneration in litchi (Litchi chinensis Sonn.)

Guo

Wang

Liu

et al. 2022

Preprint

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Litchi (Litchi chinensis Sonn.), like other ligneous plants, has long been considered a recalcitrant embryogenic species. Our previous research has shown that adding putrescine (Put) or D-Arginine (D-Arg) to the subculturing medium of embryonic callus (EC) affects EC proliferation and subsequent somatic embryogenesis (SE) in litchi. In this paper, we further confirmed that EC proliferation was significantly increased when either 0.17 mM putrescine (P3) or 2 mM D-Arginine (Ar3) was added to the control medium (M3, MS supplement with 4.52 µM 2,4-D). The subsequent induction of opalescent embryos (OEs) and opalescent dicotyledonous embryos (ODEs) was partially inhibited by Put, and the number of plantlets germinated from the OEs on the P3 medium were lower than those on the M3 medium; however, that was increased by D-Arg. Histomorphological analyses verified various developmental stages of EC proliferation in the M3, P3, and Ar3 media. On the M3 medium, an EC cell was divided into two cells first and then sequentially differentiated through multicell proembryo, globular, heart-shaped, and cotyledonary embryo stages. The EC cells on P3 and Ar3 medium were enlarged more significantly, undergoing repeated cell divisions and then forming a meristematic mass, from which OEs were initiated. The supplementation of Put into the M3 medium promoted the synthesis of endogenous Put and its conversion to Spd and Spm. The PA content in EC on the P3 medium was higher than that on the M3 medium, and the P3 medium enhanced the activity of arginine decarboxylase (ADC), ornithine decarboxylase (ODC), and diamine oxidase (DAO); however, it decreased the activity of polyamine oxidase (PAO). The PA content in the Ar3 medium was higher than that in the M3 medium. The supplementation of D-Arg to the M3 medium enhanced ADC and DAO activity but decreased ODC and PAO activity. In the other experiment, EC from the P3 medium was subsequently cultured on M3 (P3M3), P3 (P3P3), and Ar3 (P3Ar3) medium, using EC from M3 medium, and then cultured on M3 (M3M3) medium as a control. The EC proliferation rate of the P3Ar3 treatment was significantly higher than that of the other two treatments. The OEs, ODEs, and plantlets were all most elevated in the P3Ar3 treatment, followed by the P3M3 and P3P3 treatments. EC proliferation and plantlets were significantly higher than in the M3M3 treatment. When ECs were first cultured on Ar3 medium and transferred to M3 (Ar3M3), P3 (Ar3P3), and Ar3 (Ar3Ar3) media for 20 d, the EC proliferation of the Ar3Ar3 treatment was significantly higher than that of the other two treatments. Among the three treatments, EC from the Ar3Ar3 treatment showed the highest OE, ODE, and plantlet induction, followed by the Ar3M3 and P3P3 treatments. The Ar3Ar3 treatment also had the highest induction of OEs, ODEs, and plantlets compared with the other six treatments. EC from the Ar3P3 treatment had the highest Spd, Spm, and PA content, and the M3M3 treatment had the highest Put content. The Spm and PA content of EC from the M3M3 treatment was lower than in the other treatments. Among all treatments, the highest ADC, ODC, DAO, and PAO activity was found in ECs from the M3M3, P3P3, P3P3, and Ar3Ar3 treatments. These results indicate that exogenous Put or D-Arg could stimulate Put synthesis of endogenous Put by regulating the enzyme activities and then affecting the EC proliferation and SE of litchi.

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Section: Discussionmentioning

confidence: 54%

Influence of exogenous putrescine on somatic embryogenesis and regeneration in litchi (Litchi chinensis Sonn.)

Guo

Wang

Liu

et al. 2022

Preprint

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“…PAs, as one of the N metabolites, are another strategy for plants to resist stress by synthesizing Arg to reduce ammonium toxicity. 36 When plants encounter environmental stress, cells play a protective role by balancing PAs content. 36 Commonly, PAs are Put, cadaverine, spd, and Spm.…”

Section: Nomentioning

confidence: 99%

“…36 When plants encounter environmental stress, cells play a protective role by balancing PAs content. 36 Commonly, PAs are Put, cadaverine, spd, and Spm. On the one hand, PAs are not only free radical scavengers due to their unique polycationic structure but also they can activate antioxidant enzymes, such as APX, CAT, and SOD.…”

Section: Nomentioning

confidence: 99%

Endophytic Bacillus pumilus G5 Interacting with Silicon to Improve Drought Stress Resilience in Glycyrrhiza uralensis Fisch. by Modulating Nitrogen Absorption, Assimilation, and Metabolism Pathways

Xu,

Bai,

Peng

et al. 2024

J. Agric. Food Chem.

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Drought stress has become the primary severe threat to global agriculture production, including medicinal plants. Plant growth-promoting bacteria (PGPB) and environmentally friendly element silicon (Si) have emerged as effective methods in alleviating drought stress in various plants. Here, the effects of the plant endophytic G5 interaction with Si on regulating nitrogen absorption, assimilation, and metabolism pathways were investigated in the morphophysiological and gene attributes of Glycyrrhiza uralensis exposed to drought. Results showed that G5+Si application improved nitrogen absorption and assimilation by increasing the available nitrogen content in the soil, further improving the nitrogen utilization efficiency. Then, G5+Si triggered the accumulation of the major adjustment substances proline, γ-aminobutyric acid, putrescine, and chlorophyll, which played an important role in contributing to maintaining balance and energy supply in G. uralensis exposed to drought. These findings will provide new ideas for the combined application of PGPR and Si on both soil and plant systems in a drought habitat.

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“…At present, exogenous polyamines and polyamine inhibitors have been studied under abiotic stresses, such as low-temperature stress [ 26 , 27 ], salt stress [ 28 ], and drought stress [ 29 ], and under high-temperature stresses, exogenous polyamines and polyamine inhibitors have also been reported in rice [ 30 ], apricot [ 31 ], and sweet orange [ 32 ]. However, as we know, the effects of exogenous polyamines and the polyamine inhibitor D-arg on lettuce under high-temperature stress have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Role of Spermidine in Photosynthesis and Polyamine Metabolism in Lettuce Seedlings under High-Temperature Stress

Hao

et al. 2022

Plants

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High temperature is a huge threat to lettuce production in the world, and spermidine (Spd) has been shown to improve heat tolerance in lettuce, but the action mechanism of Spd and the role of polyamine metabolism are still unclear. The effects of Spd and D-arginine (D-arg) on hydroponic lettuce seedlings under high-temperature stress by foliar spraying of Spd and D-arg were investigated. The results showed that high-temperature stress significantly inhibited the growth of lettuce seedlings, with a 33% decrease in total fresh weight and total dry weight; photosynthesis of lettuce seedlings was inhibited by high-temperature stress, and the inhibition was greater in the D-arg treatment, while the Spd recovery treatment increased net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), stomatal limit value (Ls), and intercellular CO2 concentration (Ci). High-temperature stress significantly reduced the maximum photochemical efficiency (Fv/Fm), photochemical quenching coefficient (qP), electron transport rate (ETR), and photochemical efficiency of PSII (ΦPSII), increased the non-photochemical burst coefficient (NPQ) and reduced the use of light energy, which was alleviated by exogenous Spd. The increase in polyamine content may be due to an increase in polyamine synthase activity and a decrease in polyamine oxidase activity, as evidenced by changes in the expression levels of genes related to polyamine synthesis and metabolism enzymes. This evidence suggested that D-arg suppressed endogenous polyamine levels in lettuce and reduced its tolerance, whereas exogenous Spd promoted the synthesis and accumulation of polyamines in lettuce and increased its photosynthetic and oxidative stress levels, which had an impact on the tolerance of lettuce seedlings.

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Exogenous putrescine activates the arginine-polyamine pathway and inhibits the decomposition of endogenous polyamine in Anthurium andraeanum under chilling stress

Cited by 11 publications

References 28 publications

Influence of exogenous putrescine on somatic embryogenesis and regeneration in litchi (Litchi chinensis Sonn.)

Influence of exogenous putrescine on somatic embryogenesis and regeneration in litchi (Litchi chinensis Sonn.)

Endophytic Bacillus pumilus G5 Interacting with Silicon to Improve Drought Stress Resilience in Glycyrrhiza uralensis Fisch. by Modulating Nitrogen Absorption, Assimilation, and Metabolism Pathways

Role of Spermidine in Photosynthesis and Polyamine Metabolism in Lettuce Seedlings under High-Temperature Stress

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