Exogenous Strigolactone (GR24) Positively Regulates Growth, Photosynthesis, and Improves Glandular Trichome Attributes for Enhanced Artemisinin Production in Artemisia annua

Wani, Kaiser Iqbal; Zehra, Andleeb; Choudhary, Sadaf; Naeem, M.; Khan, M. Masroor A.; Khan, Ruby; Aftab, Tariq

doi:10.1007/s00344-022-10654-w

Cited by 27 publications

(17 citation statements)

References 66 publications

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“…However, far fewer studies have been reported about SL effects on the outgrowth of axillary buds in ratoon rice. GR24, a synthetic SL analog, has been widely used to study multiple pathways by which SL affects various aspects of plant growth and development [ 18 , 19 , 20 , 24 ]. In this study, we observed that after treatment with GR24, SL significantly inhibits the ratoon rate ( Figure 1 A–D), indicating that SL is involved in the growth regulation of the middle and upper axillary buds of ratoon rice, and that its mechanism is similar to that of tiller regulation.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals Inhibitory Roles of Strigolactone in Axillary Bud Outgrowth in Ratoon Rice

Ku,

Su,

Peng

et al. 2024

Plants

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Axillary bud outgrowth, a key factor in ratoon rice yield formation, is regulated by several phytohormone signals. The regulatory mechanism of key genes underlying ratoon buds in response to phytohormones in ratoon rice has been less reported. In this study, GR24 (a strigolactone analogue) was used to analyze the ratooning characteristics in rice cultivar Huanghuazhan (HHZ). Results show that the elongation of the axillary buds in the first seasonal rice was significantly inhibited and the ratoon rate was reduced at most by up to 40% with GR24 treatment. Compared with the control, a significant reduction in the content of auxin and cytokinin in the second bud from the upper spike could be detected after GR24 treatment, especially 3 days after treatment. Transcriptome analysis suggested that there were at least 742 and 2877 differentially expressed genes (DEGs) within 6 h of GR24 treatment and 12 h of GR24 treatment, respectively. Further bioinformatics analysis revealed that GR24 treatment had a significant effect on the homeostasis and signal transduction of cytokinin and auxin. It is noteworthy that the gene expression levels of OsCKX1, OsCKX2, OsGH3.6, and OsGH3.8, which are involved in cytokinin or auxin metabolism, were enhanced by the 12 h GR24 treatment. Taken overall, this study showed the gene regulatory network of auxin and cytokinin homeostasis to be regulated by strigolactone in the axillary bud outgrowth of ratoon rice, which highlights the importance of these biological pathways in the regulation of axillary bud outgrowth in ratoon rice and would provide theoretical support for the molecular breeding of ratoon rice.

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Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals Inhibitory Roles of Strigolactone in Axillary Bud Outgrowth in Ratoon Rice

Ku,

Su,

Peng

et al. 2024

Plants

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“…GR24 application increased the activity and gene expression of antioxidant enzymes, and it reduced malonaldehyde (MDA) and hydrogen peroxide (H 2 O 2 ) content in Low Light stressed plants (LL-stressed plants) [44,45]. These results suggested that exogenous application of GR24 enhances plant tolerance to LL and improving photosynthesis by promoting utilization of light energy to alleviate photosystem injuries induced by excess light energy and ROS as well as enhancing photosynthesis efficiency to improve plant growth [45,46]. Exogenous GR24 application on tomato seedlings reduces the negative effects of low light exposure in at least three ways: by reducing growth inhibition, improving photosynthetic efficiency, and relieving oxidative stress [45,47,48].…”

Section: Impact On Low-light Stressmentioning

confidence: 94%

Strigolactones in Plants and Their Interaction with the Ecological Microbiome in Response to Abiotic Stress

Soliman

Wang

Han

et al. 2022

Plants

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Phytohormones play an essential role in enhancing plant tolerance by responding to abiotic stresses, such as nutrient deficiency, drought, high temperature, and light stress. Strigolactones (SLs) are carotenoid derivatives that occur naturally in plants and are defined as novel phytohormones that regulate plant metabolism, growth, and development. Strigolactone assists plants in the acquisition of defensive characteristics against drought stress by initiating physiological responses and mediating the interaction with soil microorganisms. Nutrient deficiency is an important abiotic stress factor, hence, plants perform many strategies to survive against nutrient deficiency, such as enhancing the efficiency of nutrient uptake and forming beneficial relationships with microorganisms. Strigolactone attracts various microorganisms and provides the roots with essential elements, including nitrogen and phosphorus. Among these advantageous microorganisms are arbuscular mycorrhiza fungi (AMF), which regulate plant metabolic activities through phosphorus providing in roots. Bacterial nodulations are also nitrogen-fixing microorganisms found in plant roots. This symbiotic relationship is maintained as the plant provides organic molecules, produced in the leaves, that the bacteria could otherwise not independently generate. Related stresses, such as light stress and high-temperature stress, could be affected directly or indirectly by strigolactone. However, the messengers of these processes are unknown. The most prominent connector messengers have been identified upon the discovery of SLs and the understanding of their hormonal effect. In addition to attracting microorganisms, these groups of phytohormones affect photosynthesis, bridge other phytohormones, induce metabolic compounds. In this article, we highlighted the brief information available on SLs as a phytohormone group regarding their common related effects. In addition, we reviewed the status and described the application of SLs and plant response to abiotic stresses. This allowed us to comprehend plants’ communication with the ecological microbiome as well as the strategies plants use to survive under various stresses. Furthermore, we identify and classify the SLs that play a role in stress resistance since many ecological microbiomes are unexplained.

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“…Carbonic anhydrase (CA) activity in fresh leaves was measured using the protocol developed by Wani et al. (2023) .…”

Section: Methodsmentioning

confidence: 99%

“…Carbonic anhydrase (CA) activity in fresh leaves was measured using the protocol developed by Wani et al (2023). Fresh leaf sample (0.2 g) devoid of veins were sliced into small rectangular pieces and submerged in 10 mL of 0.2 M aqueous cysteine hydrochloride solution.…”

Section: Carbonic Anhydrase (Ca) and Nitrate Reductase (Nr) Activitiesmentioning

confidence: 99%

Enhancing growth, vitality, and aromatic richness: unveiling the dual magic of silicon dioxide and titanium dioxide nanoparticles in Ocimum tenuiflorum L.

Bhat,

Uddin,

Chishti

et al. 2024

Front. Plant Sci.

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Ocimum tenuiflorum, commonly known as “Holy basil,” is renowned for its notable medicinal and aromatic attributes. Its unique fragrance attributes to specific volatile phytochemicals, primarily belonging to terpenoid and/or phenylpropanoid classes, found within their essential oils. The use of nanoparticles (NPs) in agriculture has attracted attention among plant researchers. However, the impact of NPs on the modulation of morpho-physiological aspects and essential oil production in medicinal plants has received limited attention. Consequently, the present study aimed to explore the effect of silicon dioxide (SiO2) and titanium dioxide (TiO2) nanoparticles at various concentrations (viz., DDW (control), Si50+Ti50, Si100+Ti50, Si100+Ti100, Si200+Ti100, Si100+Ti200 and Si200+Ti200 mg L-1) on growth, physiology and essential oil production of O. tenuiflorum at 120 days after planting (DAP). The results demonstrated that the combined application of Si and Ti (Si100+Ti100 mg L-1) exhibited the most favourable outcomes compared to the other combinational treatments. This optimal treatment significantly increased the vegetative growth parameters (root length (33.5%), shoot length (39.2%), fresh weight (62.7%) and dry weight (28.5%)), photosynthetic parameters, enzymatic activities (nitrate reductase and carbonic anhydrase), the overall area of PGTs (peltate glandular trichomes) and essential oil content (172.4%) and yield (323.1%), compared to the control plants. Furthermore, the GCMS analysis showed optimal treatment (Si100+Ti100) significantly improved the content (43.3%) and yield (151.3%) of eugenol, the primary active component of the essential oil. This study uncovers a remarkable and optimal combination of SiO2 and TiO2 nanoparticles that effectively enhances the growth, physiology, and essential oil production in Holy basil. These findings offer valuable insights into maximizing the potential benefits of its use in industrial applications.

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Exogenous Strigolactone (GR24) Positively Regulates Growth, Photosynthesis, and Improves Glandular Trichome Attributes for Enhanced Artemisinin Production in Artemisia annua

Cited by 27 publications

References 66 publications

Comparative Transcriptome Analysis Reveals Inhibitory Roles of Strigolactone in Axillary Bud Outgrowth in Ratoon Rice

Comparative Transcriptome Analysis Reveals Inhibitory Roles of Strigolactone in Axillary Bud Outgrowth in Ratoon Rice

Strigolactones in Plants and Their Interaction with the Ecological Microbiome in Response to Abiotic Stress

Enhancing growth, vitality, and aromatic richness: unveiling the dual magic of silicon dioxide and titanium dioxide nanoparticles in Ocimum tenuiflorum L.

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