Exogenous sucrose increases chilling tolerance in cucumber seedlings by modulating antioxidant enzyme activity and regulating proline and soluble sugar contents

Cao, Yanyan; Yang, Mei‐Sang; Xue, Li; Zhou, Zi-Qing; Wang, Xiujuan; Bai, Ji-Gang

doi:10.1016/j.scienta.2014.09.016

Cited by 49 publications

(21 citation statements)

References 47 publications

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“…The disassembly and modification of the cell wall under DT not only leads to ending the life of a cell in senescing leaves, but breaks down polysaccharides to soluble sugars, such as glucose and sucrose (Tenhaken, 2014). The presence of sufficient sucrose can serve as the important energy source for the cells, and they act as an essential osmoprotectant to protect biomembranes and proteins against abiotic stress (Cao et al, 2014). In addition, sucrose synthesis related genes were exclusively induced during CT, while sucrose degradation-related genes were more activated during DT than during CT and CD.…”

Section: Discussionmentioning

confidence: 99%

Global Transcriptional Analysis Reveals the Complex Relationship between Tea Quality, Leaf Senescence and the Responses to Cold-Drought Combined Stress in Camellia sinensis

et al. 2016

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In field conditions, especially in arid and semi-arid areas, tea plants are often simultaneously exposed to various abiotic stresses such as cold and drought, which have profound effects on leaf senescence process and tea quality. However, most studies of gene expression in stress responses focus on a single inciting agent, and the confounding effect of multiple stresses on crop quality and leaf senescence remain unearthed. Here, global transcriptome profiles of tea leaves under separately cold and drought stress were compared with their combination using RNA-Seq technology. This revealed that tea plants shared a large overlap in unigenes displayed “similar” (26%) expression pattern and avoid antagonistic responses (lowest level of “prioritized” mode: 0%) to exhibit very congruent responses to co-occurring cold and drought stress; 31.5% differential expressed genes and 38% of the transcriptome changes in response to combined stresses were unpredictable from cold or drought single-case studies. We also identified 319 candidate genes for enhancing plant resistance to combined stress. We then investigated the combined effect of cold and drought on tea quality and leaf senescence. Our results showed that drought-induced leaf senescence were severely delayed by (i) modulation of a number of senescence-associated genes and cold responsive genes, (ii) enhancement of antioxidant capacity, (iii) attenuation of lipid degradation, (iv) maintenance of cell wall and photosynthetic system, (v) alteration of senescence-induced sugar effect/sensitivity, as well as (vi) regulation of secondary metabolism pathways that significantly influence the quality of tea during combined stress. Therefore, care should be taken when utilizing a set of stresses to try and maximize leaf longevity and tea quality.

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Section: Discussionmentioning

confidence: 99%

Global Transcriptional Analysis Reveals the Complex Relationship between Tea Quality, Leaf Senescence and the Responses to Cold-Drought Combined Stress in Camellia sinensis

et al. 2016

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“…For the expression profiles of individual genes, the names of the genes and sample data are shown in each histogram, and the genes were added to the sugar metabolism network, which we first presented in this study. Three replicates were performed, and the data are presented as the means ± standard errors important osmoprotectants against injuries to the membrane, and they not only are the primary energy source for the cells but also play certain important roles, for instance, they might involve in reactive oxygen species (especially of hydroxyl radicals) scavenging in response to stress (Bolouri-Moghaddam et al 2010;Cao et al 2014;Huang et al 2013;Keunen et al 2013;Valluru et al 2008;Van den Ende 2013). In addition, the results showed that Suc was the most abundant of TSS whose content was significantly increased with the decrease of air temperature during CA, which suggested that Suc was fundamental for tea plant respond to cold stimulation (Table 1; Fig.…”

Section: Sugar Accumulated Through the Induction Of Sugar Metabolism mentioning

confidence: 99%

Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season

et al. 2015

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Sugar plays an essential role in plant cold acclimation (CA), but the interaction between CA and sugar remains unclear in tea plants. In this study, during the whole winter season, we investigated the variations of sugar contents and the expression of a large number of sugar-related genes in tea leaves. Results indicated that cold tolerance of tea plant was improved with the development of CA during early winter season. At this stage, starch was dramatically degraded, whereas the content of total sugars and several specific sugars including sucrose, glucose and fructose were constantly elevated. Beyond the CA stage, the content of starch was maintained at a low level during winter hardiness (WH) period and then was elevated during de-acclimation (DC) period. Conversely, the content of sugar reached a peak at WH stage followed by a decrease during DC stage. Moreover, gene expression results showed that, during CA period, sugar metabolism-related genes exhibited different expression pattern, in which beta-amylase gene (CsBAM), invertase gene (CsINV5) and raffinose synthase gene (CsRS2) engaged in starch, sucrose and raffinose metabolism respectively were solidly up-regulated; the expressions of sugar transporters were stimulated in general except the down-regulations of CsSWEET2, 3, 16, CsERD6.7 and CsINT2; interestingly, the sugar-signaling related CsHXK3 and CsHXK2 had opposite expression patterns at the early stage of CA. These provided comprehensive insight into the effects of CA on carbohydrates indicating that sugar accumulation contributes to tea plant cold tolerance during winter season, and a simply model of sugar regulation in response to cold stimuli is proposed.

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“…The ICE-CBF-COR regulatory pathway is one of the major ways how plants acquire resistance to cold [44,45]. Plants can also protect their membrane structures and reduce cold damage through the synergistic action of superoxide dismutase (SOD), peroxidase (POD) protective enzymes and sugar metabolism [46,47]. Our results suggest that AS may play crucial regulatory roles in these pathways in order to enhance the cold resistance of plants.…”

Section: Discussionmentioning

confidence: 87%

Comprehensive profiling of alternative splicing landscape during cold acclimation in tea plant

Zhao

et al. 2020

BMC Genomics

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Background: Alternative splicing (AS) may generate multiple mRNA splicing isoforms from a single mRNA precursor using different splicing sites, leading to enhanced diversity of transcripts and proteins. AS has been implicated in cold acclimation by affecting gene expression in various ways, yet little information is known about how AS influences cold responses in tea plant (Camellia sinensis).Results: In this study, the AS transcriptional landscape was characterized in the tea plant genome using highthroughput RNA-seq during cold acclimation. We found that more than 41% (14,103) of genes underwent AS events. We summarize the possible existence of 11 types of AS events, including the four common types of intron retention (IR), exon skipping (ES), alternative 5′ splice site (A5SS), and alternative 3′ splice site (A3SS); of these, IR was the major type in all samples. The number of AS events increased rapidly during cold treatment, but decreased significantly following de-acclimation (DA). It is notable that the number of differential AS genes gradually increased during cold acclimation, and these genes were enriched in pathways relating to oxidoreductase activity and sugar metabolism during acclimation and de-acclimation. Remarkably, the AS isoforms of bHLH transcription factors showed higher expression levels than their full-length ones during cold acclimation. Interestingly, the expression pattern of some AS transcripts of raffinose and sucrose synthase genes were significantly correlated with sugar contents. Conclusion: Our findings demonstrated that changes in AS numbers and transcript expression may contribute to rapid changes in gene expression and metabolite profile during cold acclimation, suggesting that AS events play an important regulatory role in response to cold acclimation in tea plant.

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Exogenous sucrose increases chilling tolerance in cucumber seedlings by modulating antioxidant enzyme activity and regulating proline and soluble sugar contents

Cited by 49 publications

References 47 publications

Global Transcriptional Analysis Reveals the Complex Relationship between Tea Quality, Leaf Senescence and the Responses to Cold-Drought Combined Stress in Camellia sinensis

Global Transcriptional Analysis Reveals the Complex Relationship between Tea Quality, Leaf Senescence and the Responses to Cold-Drought Combined Stress in Camellia sinensis

Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season

Comprehensive profiling of alternative splicing landscape during cold acclimation in tea plant

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