Exopolysaccharide from surface-liquid culture of Clonostachys rosea originates from autolysis of the biomass

Viccini, Graciele; Martinelli, Thalita Romano; Cognialli, Regielly Caroline Raimundo; Faria, Rodrigo Otávio de; Carbonero, Elaine R.; Sassaki, Guilherme L.; Mitchell, David A.

doi:10.1007/s00203-009-0464-0

Cited by 16 publications

(14 citation statements)

References 46 publications

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“…Examination of the 2D NMR spectrum profile for MM-grown samples showed major differences especially regarding the presence of anomeric signals attributed to α-D-Man p units, at δ 101.1/5.22 (Figures 2A,C) (Barreto-Bergter et al, 2008). On CD medium, the differentiation of the strains were related to α-D-Man p (1→6) and β-D-Man p (1→2) (Figures 2B,D), (Viccini et al, 2009). …”

Section: Resultsmentioning

confidence: 88%

“…The main difference found for this strain when comparing growth condition is the presence of signals corresponding to β-D-Gal f units (δ 106.0/5.23 and δ 105.3/5.23), on CD medium, in contrast to the signals attributed to α-D-Man p (δ 99.1/5.11) and β-D-Man p (δ 99.1/4.82 and 101.3/5.11) (Viccini et al, 2009) found for the MM samples (Figures 2A,B). …”

Section: Resultsmentioning

confidence: 99%

“…Glucose was measured based on glucose oxidase method (DiaSys Diagnostic Systems GMBH & Co.KG), which forms gluconic acid and H 2 O 2 . The latter reacts with the phenol and 4-amineantipyrine, giving rise to p-benzoquinone-monoimine antipyrine (red color) and the concentration of residual glucose determined, in mg/ml, by the absorbance measured at 500 nm (Viccini et al, 2009). Sucrose consumption was determined by Silica-Gel 60G TLC plates (Merck), with sucrose solutions in different concentrations as standard (2.5–10 mg.ml −1 ).…”

Section: Methodsmentioning

confidence: 99%

“…Secondary host infection is dependent mainly on three factors: host resistance, amount of inoculum and fungal virulence (Alviano et al, 2003). Those factors are influenced by the composition of the culture media (Viccini et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

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Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response

Burjack

Santana-Filho

Ruthes

et al. 2014

Front. Cell. Infect. Microbiol.

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Dematiaceous fungi constitute a large and heterogeneous group, characterized by having a dark pigment, the dihydroxynaftalen melanin—DHN, inside their cell walls. In nature they are found mainly as soil microbiota or decomposing organic matter, and are spread in tropical and subtropical regions. The fungus Fonsecaea monophora causes chromoblastomycosis in humans, and possesses essential mechanisms that may enhance pathogenicity, proliferation and dissemination inside the host. Glycoconjugates confer important properties to these pathogenic microorganisms. In this work, structural characterization of glycan structures present in two different strains of F. monophora MMHC82 and FE5p4, from clinical and environmental origins, respectively, was performed. Each one were grown on Minimal Medium (MM) and Czapeck-Dox (CD) medium, and the water soluble cell wall glycoconjugates and exopolysaccharides (EPS) were evaluated by NMR, methylation and principal component analysis (PCA). By combining the methylation and 2D NMR analyses, it was possible to visualize the glycosidic profiles of the complex carbohydrate mixtures. Significant differences were observed in β-D-Galf-(1→5) and (1→6) linkages, α- and β-D-Glcp-(1→3), (1→4), and (1→6) units, as well as in α-D-Manp. PCA from 1H-NMR data showed that MMHC82 from CD medium showed a higher variation in the cell wall carbohydrates, mainly related to O-2 substituted β-D-Galf (δ 106.0/5.23 and δ 105.3/5.23) units. In order to investigate the antigenic response of the glycoconjugates, these were screened against serum from chromoblastomycosis patients. The antigen which contained the cell wall of MMHC82 grown in MM had β-D-Manp units that promoted higher antigenic response. The distribution of these fungal species in nature and the knowledge of how cell wall polysaccharides and glycoconjugates structure vary, may contribute to the better understanding and the elucidation of the pathology caused by this fungus.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response

Burjack

Santana-Filho

Ruthes

et al. 2014

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

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“…1D and 2D assignments were performed using 1 H-(zg, and zgpr) and HSQC (hsqcetgpsi) programs. HSQC was acquired using 8-16 scans, respectively, per series of 2 K × 512 W data points with zero filling in F1 (4 K) prior to Fourier transformation (Viccini et al, 2009). …”

Section: Nuclear Magnetic Resonance (Nmr)mentioning

confidence: 99%

Low molecular weight heparins: Structural differentiation by spectroscopic and multivariate approaches

Lima

Farias

Rudd

et al. 2011

Carbohydrate Polymers

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a b s t r a c tVarious branded low molecular weight heparins (LMWHs) have been used for the treatment and prevention of thrombotic for over 20 years. With the introduction of generic LMWHs and the recent events involving heparin contamination, a great deal of effort is being expended in investigating ways of monitoring and regulating this class of complex drugs. In this paper, we present the characterization of different forms of LMWHs, as well as the comparison of 5 enoxaparin copies from different manufactures. The data suggests that, while some of these drugs are structurally comparable, specific analytical methods as well as biological and pharmacological tests may be used to address their similarity, quality and potential interchangeability. The proposed approach may also be useful in comparing biosimilar and branded LMWHs.

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Taxonomy, phylogeny and evolution of freshwater Hypocreomycetidae (Sordariomycetes)

Bao,

Hyde,

Maharachchikumbura

et al. 2023

Fungal Diversity

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Hypocreomycetidae is a highly diverse group with species from various habitats. This subclass has been reported as pathogenic, endophytic, parasitic, saprobic, fungicolous, lichenicolous, algicolous, coprophilous and insect fungi from aquatic and terrestrial habitats. In this study, we focused on freshwater fungi of Hypocreomycetidae which resulted 41 fresh collections from China and Thailand. Based on morphological and phylogenetic analyses, we identified 26 species that belong to two orders (Hypocreales and Microascales) and six families (Bionectriaceae, Halosphaeriaceae, Microascaceae, Nectriaceae, Sarocladiaceae and Stachybotryaceae). Ten new species are introduced and 13 new habitats and geographic records are reported. Mariannaea superimposita, Stachybotrys chartarum and S. chlorohalonatus are recollected from freshwater habitats in China. Based on phylogenetic analysis of combined LSU, ITS, SSU, rpb2 and tef1-α sequences data, Emericellopsis is transferred to Hypocreales genera incertae sedis; Pseudoacremonium is transferred to Bionectriaceae; Sedecimiella is placed in Nectriaceae; Nautosphaeria and Tubakiella are excluded from Halosphaeriaceae and placed in Microascales genera incertae sedis; and Faurelina is excluded from Hypocreomycetidae. Varicosporella is placed under Atractium as a synonym of Atractium. In addition, phylogenetic analysis and divergence time estimates showed that Ascocodina, Campylospora, Cornuvesica and Xenodactylariaceae form distinct lineages in Hypocreomycetidae and they evolved in the family/order time frame. Hence, a new order (Xenodactylariales) and three new families (Ascocodinaceae, Campylosporaceae and Cornuvesicaceae) are introduced based on phylogenetic analysis, divergence time estimations and morphological characters. Ancestral character state analysis is performed for different habitats of Hypocreomycetidae including freshwater, marine and terrestrial taxa. The result indicates that marine and freshwater fungi evolved independently from terrestrial ancestors. The results further support those early diverging clades of this subclass, mostly comprising terrestrial taxa and freshwater and marine taxa have been secondarily derived, while the crown clade (Nectriaceae) is represented in all three habitats. The evolution of various morphological adaptations towards their habitual changes are also discussed.

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Exopolysaccharide from surface-liquid culture of Clonostachys rosea originates from autolysis of the biomass

Cited by 16 publications

References 46 publications

Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response

Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response

Low molecular weight heparins: Structural differentiation by spectroscopic and multivariate approaches

Taxonomy, phylogeny and evolution of freshwater Hypocreomycetidae (Sordariomycetes)

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