Exopolysaccharide Produced by Lactobacillus Plantarum Induces Maturation of Dendritic Cells in BALB/c Mice

Yan-jun, Tang; Dong, Wei; Wan, Keyu; Zhang, Ligang; Li, Chun; Zhang, Lili; Liu, Ning

doi:10.1371/journal.pone.0143743

Cited by 33 publications

(21 citation statements)

References 44 publications

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“…This demonstrates that many LAB strains might have TLR independent health benefits. Moreover, our data suggest that every single tested LAB has a unique PRR signalling profile which might be explained by expression of different ligands on the surface of the bacteria or secretion of PRR-stimulating bactericides or other immune active bacterial products 48 49 50 51 52 .…”

Section: Discussionmentioning

confidence: 88%

Identification of TLR2/TLR6 signalling lactic acid bacteria for supporting immune regulation

Ren

Zhang

Haan

et al. 2016

Sci Rep

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Although many lactic acid bacteria (LAB) influence the consumer’s immune status it is not completely understood how this is established. Bacteria-host interactions between bacterial cell-wall components and toll-like receptors (TLRs) have been suggested to play an essential role. Here we investigated the interaction between LABs with reported health effects and TLRs. By using cell-lines expressing single or combination of TLRs, we show that LABs can signal via TLR-dependent and independent pathways. The strains only stimulated and did not inhibit TLRs. We found that several strains such as L. plantarum CCFM634, L. plantarum CCFM734, L. fermentum CCFM381, L. acidophilus CCFM137, and S. thermophilus CCFM218 stimulated TLR2/TLR6. TLR2/TLR6 is essential in immune regulatory processes and of interest for prevention of diseases. Specificity of the TLR2/TLR6 stimulation was confirmed with blocking antibodies. Immunomodulatory properties of LABs were also studied by assessing IL-10 and IL-6 secretion patterns in bacteria-stimulated THP1-derived macrophages, which confirmed species and strain specific effects of the LABs. With this study we provide novel insight in LAB specific host-microbe interactions. Our data demonstrates that interactions between pattern recognition receptors such as TLRs is species and strain specific and underpins the importance of selecting specific strains for promoting specific health effects.

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Section: Discussionmentioning

confidence: 88%

Identification of TLR2/TLR6 signalling lactic acid bacteria for supporting immune regulation

Ren

Zhang

Haan

et al. 2016

Sci Rep

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“…As a major component of the human gut microbiota, Lactobacilli generate immunomodulatory effects and can interact with antigen‐presenting cells (Braat, van den Brande, et al, 2004; Braat, de Jong, et al, 2004; Cai et al, 2016; Christensen et al, 2002; Elawadli et al, 2014; Evrard et al, 2011; Fink et al, 2007; Forsythe et al, 2007; Gorska et al, 2014; Haileselassie et al, 2016; Hart et al, 2004; Hsieh et al, 2016; Jo et al, 2016; Jones & Versalovic, 2009; Kagnoff & Eckmann, 1997; Karlsson et al, 2004; Kelsall & Strober, 1997; Ma et al, 2004; Mohamadzadeh et al, 2005; Mowat et al, 2003; Rescigno et al, 2001; Sansonetti, 2004; Tang et al, 2015; Yang et al, 2015; Zeuthen et al, 2006). Select lactobacilli stimulate the polarization of naïve immune cells, thereby modifying the immune response (Sudo et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Immunomodulation of dendritic cells byLactobacillus reuterisurface components and metabolites

et al. 2021

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“…[45][46][47] Mannose has been reported to be a minor component of the EPSs produced by LAB. 44,48,49) In addition, with respect to LAB-derived EPSs having a high mannose content, 46,50,51) the mannose content in the EPS produced by Lb. mucosae DPC 6426, Lb.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Exopolysaccharides Produced by Thermophilic Lactic Acid Bacteria Isolated from Tropical Fruits of Thailand

Panthavee

Noda

Danshiitsoodol

et al. 2017

Biological & Pharmaceutical Bulletin

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In the present study, we have obtained two exopolysaccharide (EPS)-producing thermophilic lactic acid bacteria (LAB) that were isolated from tropical fruits of Thailand. The two strains, designated LY45 and PY45, were identified as Pediococcus pentosaceus and Lactobacillus amylovorus, respectively. Both plantderived LAB strains, which produce neutral EPSs together with the acidic one, can grow vigorously at 45°C and even at 50°C. Hyaluronidase (EC 3.2.1.35), which catalyzes the degradation of hyaluronic acid, activates an inflammatory reaction. Interestingly, EPSs produced by the LY45 and PY45 strains were found to inhibit hyaluronidase activity at the same order of IC 50 values as did sodium cromoglicate and dipotassium glycyrrhizinate, which are well-known as anti-inflammatory agents. The LY45-derived neutral EPS consists of glucose and mannose as monosaccharide components, whereas the acidic one contains mainly mannose, together with glucose and galactose. On the other hand, although Lactobacillus amylovorus PY45 also produces neutral and acidic EPSs, the main monosaccharide in both EPSs is mannose, and glucose is a minor component. Furthermore, the PY45 strain may be probiotically and industrially useful because the microorganism can utilize starch and glycogen as carbon sources. Key words exopolysaccharide; Lactobacillus amylovorus; Pediococcus pentosaceus; thermophilic lactic acid bacteria; anti-inflammatory substanceTraditionally, lactic acid bacteria (LAB) have been useful for producing fermented foods, such as yogurt, kimchi, and cheese. LAB-Gram-positive bacteria-are non-pathogenic and are generally recognized as safe (GRAS) microorganisms; therefore, they have been the focus of attention for their industrial importance. 1)Microorganisms that contribute to human health are called probiotics. This word is internationally defined as "living microorganisms conferring a health benefit on the host when administered in adequate amounts."2) We have isolated many kinds of LAB strains from plant sources such as vegetables, fruits, flowers, and medicinal plants to establish a plantderived LAB library with more than 600 strains. We have demonstrated that some strains stocked in the LAB library are useful for enhancing intestinal immunity, improving liver function, and preventing metabolic syndrome. [3][4][5][6] LAB fermentation often proceeds even at low temperatures, causing the overgrowth of LABs. As the result, the degradation of flavors is proceeded and affected with taste of the fermented foods. Since thermophilic LAB strains hard to ferment at lower temperatures, therefore, if the thermophile ones are successfully isolated, they will contribute to prevent over-fermentation. In the present study, we isolated 28 strains of thermophilic LAB from tropical fruits of Thailand. In the isolates, we found two exopolysaccharide (EPS)-producing LAB strains. The LY45 strain was isolated from lychee, and the PY45 strain was obtained from pineapple. These strains were identified as Pediococcus (P.) pentosaceus and Lactob...

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Exopolysaccharide Produced by Lactobacillus Plantarum Induces Maturation of Dendritic Cells in BALB/c Mice

Cited by 33 publications

References 44 publications

Identification of TLR2/TLR6 signalling lactic acid bacteria for supporting immune regulation

Identification of TLR2/TLR6 signalling lactic acid bacteria for supporting immune regulation

Immunomodulation of dendritic cells byLactobacillus reuterisurface components and metabolites

Characterization of Exopolysaccharides Produced by Thermophilic Lactic Acid Bacteria Isolated from Tropical Fruits of Thailand

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