Exopolysaccharide production by Bacillus cereus GU812900, a fouling marine bacterium

Bragadeeswaran, S.; Jeevapriya, R.; Prabhu, Kandikere Ramaiah; Rani, S. Sophia; Priyadharsini, Sethu; Balasubramanian, T.

doi:10.5897/ajmr11.375

Cited by 41 publications

(24 citation statements)

References 20 publications

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“…Though, the isolate B. cereus RCR 08 preferred maltose for growth, it failed to utilize galactose. Different carbon sources have been utilized for EPS production by endophytes (Liu et al, 2009;Bragadeeswaran et al, 2011) and glucose and sucrose are reported to be the most suitable ones.…”

Section: Resultsmentioning

confidence: 99%

Production and partial characterization of extracellular polysaccharide from endophytic Bacillus cereus RCR 08

Mukherjee¹,

Das²,

Sharma³

et al. 2017

Biosci. Biotech. Res. Comm

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The present study focuses attention on the production of extracellular polysaccharide (EPS) by bacterial endophytes of Ricinus communis L. Among the 28 endophytic bacterial isolates screened for EPS production, a potent isolate identifi ed as Bacillus cereus RCR 08 (GenBank accession number MF159112) produced signifi cant amount of EPS in mineral salts medium under batch culture. In single factor system of analysis, glucose and ammonium chloride were most suitable carbon and nitrogen sources respectively for EPS production. Maximum growth (7.1 g/L) and EPS yield (10.24 g/L) was attained when glucose and ammonium chloride were used in the ratio of 25:1. The isolated polymer contained carbohydrate (88.8%), protein (3.18%), RNA (6.0%) as well as DNA (3.2%) and showed characteristic FTIR absorption spectrum with peaks at 3404, 2,933, 1,655, and 1,042 cm −1. The emulsifying activity of the EPS was more or less comparable with Tween 80. Though the EPS failed to show any antibacterial activity, it exhibited moderate DPPH radical scavenging activity and displayed a dose-dependent cytotoxic activity against hepato cellular carcinoma (Huh 7.5) cell line in MTT assay. A detailed physico-chemical analysis is, therefore, essential to assess the signifi cance and potential importance of this endophytic EPS in biotechnology.

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Section: Resultsmentioning

confidence: 99%

Production and partial characterization of extracellular polysaccharide from endophytic Bacillus cereus RCR 08

Mukherjee¹,

Das²,

Sharma³

et al. 2017

Biosci. Biotech. Res. Comm

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“…The FT-IR spectrum of EPS from Lactobacillus was carried out to confirm the identity of exopolysaccharides (Fig.2). FTIR spectra confirmed the presence of functional groups like carbohydrate ring, mannose and Dglucose in pyranose form [6,7] .…”

Section: Characterization Of Exopolysaccharidesmentioning

confidence: 99%

Isolation and Purification of Exopolysaccharides and Exploring Its Potential as an Excipient in the Development of Suitable Formulation

Gadad¹,

Sharma²,

Darbha³

et al. 2015

IJAPB

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Exopolysaccharides (EPS) are high-molecular-weight polymers, excreted by some microorganisms onto the outside of their cell walls. EPS are primarily composed of carbohydrate and some non-carbohydrate substituents, such as acetate, phosphate, pyruvate and succinate. The study was aimed at isolation of EPS from pseudomonas strains and milk samples and also preliminary trials were carried with sea water, sea sediment and soil samples. Based on the trial study pseudomonas and milk sample (lactobacilli) were selected for EPS production. The pseudomonas strains were then subcultured in cetrimide media and lactic acid bacteria strains were subcultured in milk media (50%) supplied with 50% carbohydrate source maintained at 20º C for 72 hours. The EPS obtained from milk sample was highly viscous in nature, good smelling and high yield. The EPS obtained from pseudomonas strains didn't show satisfactory yield. The EPS obtained from lactobacilli was characterized and used as gelling agent in formulating a Diclofenac diethylamine gel. The formulated gel was evaluated and compared to marketed formulation for gelling agent, consistency and performance.

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“…cut off 8000 dalton) against distilled water for 2 days to remove the salts from exopolymer. The weight of the exopolymer was measured after drying at 45°C (Bragadeeswaran et al, 2011).…”

Section: Extraction and Characterization Exopolymeric Substancementioning

confidence: 99%

Characterization and in vitro studies on anticancer activity of exopolymer of Bacillus thuringiensis S13

Parthiban¹,

Vignesh²,

Thirumurugan³

2014

Afr. J. Biotechnol.

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The objective of the present work was to isolate and characterize the exopolymer producer from a marine environment. The exopolymer producing strain (S13) was identified as Bacillus thuringiensis S13. Characterization of exopolymer shows the presence of brominated compound responsible for cytotoxicity on lung cancer cell line (A549) on XTT assay. An in vitro study of bacterial exopolymer shows the presence of cytotoxic effects on cell lines. Further, active compound in exopolymer responsible for cytotoxicity has to be characterized. The exopolymer produced by B. thuringiensis S13, showed potent cytotoxic effects, and could be used as therapy in cancer after further studies.

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Exopolysaccharide production by Bacillus cereus GU812900, a fouling marine bacterium

Cited by 41 publications

References 20 publications

Production and partial characterization of extracellular polysaccharide from endophytic Bacillus cereus RCR 08

Production and partial characterization of extracellular polysaccharide from endophytic Bacillus cereus RCR 08

Isolation and Purification of Exopolysaccharides and Exploring Its Potential as an Excipient in the Development of Suitable Formulation

Characterization and in vitro studies on anticancer activity of exopolymer of Bacillus thuringiensis S13

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