Exopolysaccharides from Lactobacillus plantarum NCU116 Facilitate Intestinal Homeostasis by Modulating Intestinal Epithelial Regeneration and Microbiota

Abstract

Regeneration of epithelia is crucial for maintaining the intestinal barrier and homeostasis. Our previous work showed that exopolysaccharides from Lactobacillus plantarum NCU116 (EPS116) regulated the barrier function and homeostasis of the intestine; however, the relevant mechanisms remain obscure. Therefore, we sought to explore the role of EPS116 in promoting intestinal epithelial regeneration. Our data showed that the administration of EPS116 markedly ameliorated inflammatory bowel disease-related phenotypes and promoted the regeneration of crypts in the colon of colitis mice. The results of immunofluorescence and reverse transcription-quantitative polymerase chain reaction experiments indicated that EPS116 strikingly increased the number of intestinal stem cells (ISCs) and the expression of differentiation markers for goblet cells, enterocytes, and enteroendocrine cells in the mouse colon. Intestinal microbiota analysis showed that EPS116 increased microbial populations associated with intestinal regeneration and glycan metabolism. Therefore, the present study revealed a novel model that EPS116 promoted the intestinal homeostasis through modulating the proliferation and differentiation of ISCs and altering the gut microbiota profile.

Alleviative Effects of Exopolysaccharide Produced by Lactobacillus helveticus KLDS1.8701 on Dextran Sulfate Sodium-Induced Colitis in Mice

“…In addition, DSS-induced pathological damage in the colon was also improved after EPS-1 administration. Similar effects were also found in the previous results [14,17]. Therefore, EPS-1 administration showed a good preventive effect on the DSS-induced UC.…”

“…longum YS108R could protect against the development of UC by maintenance of the mucosal barrier and gut microbiota modulation [15]. The EPS produced by L. plantarum NCU116 could regulate the barrier function, modulate the gut microbiota structure, and elevate c-Jun/Muc2 signaling pathway in an UC mouse model induced by DSS [16,17]. Compared to the traditional drugs, LAB-produced EPS would likely possess beneficial effects and further avoid the severe side effects.…”

Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains

“…Lactic acid bacteria have gained substantial attention in maintaining intestinal homeostasis [ 26 ]. For a new probiotic strain, stable adhesion to the intestinal epithelium is one of the crucial criteria for evaluating probiotic action, which contributes to the competition between probiotics and pathogenic bacteria for the adhesion to intestinal epithelium [ 19 ].…”

Exopolysaccharide Produced by Lactiplantibacillus plantarum-12 Alleviates Intestinal Inflammation and Colon Cancer Symptoms by Modulating the Gut Microbiome and Metabolites of C57BL/6 Mice Treated by Azoxymethane/Dextran Sulfate Sodium Salt

“…Studies have revealed that Staphylococcaceae was consistently augmented in IBD and colon cancer [ 2 , 23 ]. Zhou et al reported that exopolysaccharides produced by Lactiplantibacillus plantarum NCU116 significantly decreased the relative abundance of Staphylococcaceae in the fecal of DSS-induced C57BL/6 mice [ 41 ]. Our results suggested that oral LPEPS were beneficial to maintain the gut microbiota balance and promote intestinal health of the C57BL/6 mice treated by AOM/DSS.…”