Exopolysaccharides Produced by <i>Lactobacillus rhamnosus</i> KL 53A and <i>Lactobacillus casei</i> Fyos Affect Their Adhesion to Enterocytes

Konieczna, Corinna; Słodziński, Michał; Schmidt, Marcin

doi:10.21307/pjm-2018-032

Cited by 14 publications

(6 citation statements)

References 27 publications

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“…However, Lactobacillus EPS was found to promote more the biofilm biomass than the cell viability in biofilms, thus it is likely that other mechanisms are also implicated. Some authors reported that L. rhamnosusderived EPS was involved in the adhesion to intestinal cells, suggesting that from lactobacilli can act as adhesins for Lactobacillus and related genera [35]. At the same time, since EPS are one of the main components of the biofilm extracellular matrix, their availability in the medium can enhance the structure of lactobacilli biofilms.…”

Section: Discussionmentioning

confidence: 99%

Exopolysaccharides from vaginal lactobacilli modulate microbial biofilms

et al. 2023

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Background Exopolysaccharides (EPS) secreted by beneficial lactobacilli exert a plethora of positive activities, but little is known about their effects on biofilms of opportunistic vaginal pathogens and especially on biofilms of lactobacilli themselves. Here, the EPS produced by six vaginal lactobacilli, belonging to Lactobacillus crispatus (BC1, BC4, BC5) and Lactobacillus gasseri (BC9, BC12, BC14) species were isolated from cultural supernatants and lyophilized. Results Lactobacillus EPS were chemically characterized in terms of monosaccharide composition by liquid chromatography (LC) analysis coupled to UV and mass spectrometry (MS) detection. Moreover, the ability of EPS (0.1, 0.5, 1 mg/mL) to stimulate the biofilm formation of lactobacilli and to inhibit the formation of pathogens’ biofilms was evaluated by crystal violet (CV) staining and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Isolated EPS (yields 133–426 mg/L) were heteropolysaccharides mainly composed of d-mannose (40–52%) and d-glucose (11–30%). For the first time we demonstrated that Lactobacillus EPS were able to stimulate in a dose-dependent manner (p < 0.05) the formation of biofilms of ten strains belonging to L. crispatus, L. gasseri and Limosilactobacillus vaginalis species, in terms of cell viability (84–282% increase at 1 mg/mL) and especially biofilm biomass (40–195% increase at 1 mg/mL), quantified with MTT assay and CV staining, respectively. EPS released from L. crispatus and L. gasseri were found to better stimulate the biofilms of the same producer species rather than that of other species, including producing strains themselves and other strains. Conversely, the biofilm formation of bacterial (Escherichia coli, Staphylococcus spp., Enterococcus spp. and Streptococcus agalactiae) and fungal (Candida spp.) pathogens was inhibited. The anti-biofilm activity was dose-dependent and was more marked for L. gasseri-derived EPS (inhibition up to 86%, 70%, and 58% at 1 mg/mL, 0.5 mg/mL, and 0.1 mg/mL, respectively), whilst L. crispatus-derived EPS resulted overall less efficient (inhibition up to 58% at 1 mg/mL and 40% at 0.5 mg/mL) (p < 0.05). Conclusions Lactobacilli-derived EPS favour the biofilm formation of lactobacilli preventing, at the same time, that of opportunistic pathogens. These results support the possible employment of EPS as postbiotics in medicine as a therapeutic/preventive strategy to counteract vaginal infections.

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Section: Discussionmentioning

confidence: 99%

Exopolysaccharides from vaginal lactobacilli modulate microbial biofilms

et al. 2023

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“…Exopolysaccharides might facilitate interactions between the bacteria and the intestinal cells, from initial to permanent adhesion mechanisms (Konieczna et al, 2018). For example, Bifidobacterium longum NB667 contains exopolysaccharides found directly involved in their adhesion to human intestinal mucus.…”

Section: Probiotic Colonization On Gastrointestinal Epithelial Cellsmentioning

confidence: 99%

Probiotics: Health benefits, food application, and colonization in the human gastrointestinal tract

Lau,

Quek

2024

Food Bioengineering

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Probiotics have become increasingly popular over the past two decades due to the continuously expanding scientific evidence indicating their beneficial effects on human health. Therefore, they have been applied in the food industry to produce functional food, which plays a significant role in human health and reduces disease risk. However, maintaining the viability of probiotics and targeting the successful delivery to the gastrointestinal tract remain two challenging tasks in food applications. Specifically, this paper reviews the potentially beneficial properties of probiotics, highlighting the use and challenges of probiotics in food application and the associated health benefits. Of foremost importance, this paper also explores the potential underlying molecular mechanisms of the enhanced effect of probiotics on gastrointestinal epithelial cells, including a discussion on various surface adhesion‐related proteins on the probiotic cell surface that facilitate colonization.

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“… 154 Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos EPS Enzymatic deglycosylation Changes in the exopolysaccharide structure decreased adhesion efficiency of probiotic on IECs. 155 Lactobacillus acidophilus NCFM SLP homolog PrtX PrtX-deficient strain (gene deletion) Prt-X deficient strain had increased adhesion to mucin and fibronectin. On antigen presenting cells (DCs) it increased induction of IL-6, IL-12 (pro-inflammatory) and IL-10 (anti-inflammatory), but IL-10/IL-12 ratio, (measure of the balance between pro-inflammatory and anti-inflammatory states) was higher.…”

Section: Evidence Of Involvement Of Adhesins In Probiotic Function By...mentioning

confidence: 99%

The potential role of adherence factors in probiotic function in the gastrointestinal tract of adults and pediatrics: a narrative review of experimental and human studies

Gorreja

Walker

2022

Gut Microbes

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Numerous studies point to the important role of probiotic bacteria in gastrointestinal health. Probiotics act through mechanisms affecting enteric pathogens, epithelial barrier function, immune signaling, and conditioning of indigenous microbiota. Once administered, probiotics reach the gastrointestinal tract and interact with the host through bacterial surface molecules, here called adhesion factors, which are either strain- or specie-specific. Probiotic adhesion, through structural adhesion factors, is a mechanism that facilitates persistence within the gastrointestinal tract and triggers the initial host responses. Thus, an understanding of specific probiotic adhesion mechanisms could predict how specific probiotic strains elicit benefits and the potential of adherence factors as a proxy to predict probiotic function. This review summarizes the present understanding of probiotic adherence in the gastrointestinal tract. It highlights the bacterial adhesion structure types, their molecular communication with the host and the consequent impact on intestinal diseases in both adult and pediatric populations. Finally, we discuss knockout/isolation studies as direct evidence for adhesion factors conferring anti-inflammatory and pathogen inhibition properties to a probiotic. What is known : Probiotics can be used to treat clinical conditions. Probiotics improve dysbiosis and symptoms. Clinical trials may not confirm in vitro and animal studies. What is new : Adhesion structures may be important for probiotic function. Need to systematically determine physical characteristics of probiotics before selecting for clinical trials. Probiotics may be genetically engineered to add to clinical efficacy.

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Exopolysaccharides Produced by Lactobacillus rhamnosus KL 53A and Lactobacillus casei Fyos Affect Their Adhesion to Enterocytes

Cited by 14 publications

References 27 publications

Exopolysaccharides from vaginal lactobacilli modulate microbial biofilms

Exopolysaccharides from vaginal lactobacilli modulate microbial biofilms

Probiotics: Health benefits, food application, and colonization in the human gastrointestinal tract

The potential role of adherence factors in probiotic function in the gastrointestinal tract of adults and pediatrics: a narrative review of experimental and human studies

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