Exosomal circPACRGL promotes progression of colorectal cancer via the miR-142-3p/miR-506-3p- TGF-β1 axis

Shang, Anquan; Gu, Chenzheng; Wang, Weiwei; Wang, Xuan; Sun, Junjun; Zeng, Bingjie; Chen, Chen; Chang, Wenjing; Ping, Yili; Ji, Ping; Wu, Junlu; Quan, Wenqiang; Yao, Yiwen; Zhou, Yilu; Sun, Zujun; Dong, Li

doi:10.1186/s12943-020-01235-0

Cited by 326 publications

(235 citation statements)

References 27 publications

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“…CircPACRGL promoted the proliferation, migration and invasion of CRC cells and the differentiation of Neutrophils from N1 to N2 via miR-142-3p/miR-506-3P-TGF-1 axis. This study is the first to reveal the role of cancer-derived exosomal circPACRGL in CRC proliferation and metastasis (Shang et al, 2020).…”

Section: Exosomal Circrnas May Be Therapeutic Targets For Inhibitingmentioning

confidence: 84%

Exosomal circRNAs: Sorting Mechanisms, Roles and Clinical Applications in Tumors

Yan

Kong

Qin

et al. 2020

Front. Cell Dev. Biol.

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Exosomes are a group of nano-sized membrane vesicles and are important mediators of intercellular communication, particularly in tumor microenvironment. Recently, researchers have found that circular RNAs (circRNAs), with the great research significance, are enriched and stable in exosomes. In this review, we summarize the research significance of exosomal circRNAs, sorting mechanisms and their functioning mechanisms in tumor progression. Their clinical applications as clinical tumor biomarkers and as therapeutic targets in inhibiting tumor metastasis, anti-cancer immunity response and drug resistance have been widely discussed.

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Section: Exosomal Circrnas May Be Therapeutic Targets For Inhibitingmentioning

confidence: 84%

Exosomal circRNAs: Sorting Mechanisms, Roles and Clinical Applications in Tumors

Yan

Kong

Qin

et al. 2020

Front. Cell Dev. Biol.

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“…For instance, exosomal circPACRGL contributed to the progression of CRC by regulating miR-142-3p/miR-506-3p-TGF-β1 axis. 41 Exosome-delivered hsa_circ_0005963 facilitated glycolysis to induced chemoresistance by the miR-122/PKM2 axis in CRC. 42 A previous report showed that circ_IFT80 expression was upregulated in CRC serum exosomes, CRC tissues and CRC cells, circ_IFT80 silencing repressed cell proliferation while accelerated apoptosis through miR-1236-3p/HOXB7 axis in CRC.…”

Section: Discussionmentioning

confidence: 99%

Exosomal circ_IFT80 Enhances Tumorigenesis and Suppresses Radiosensitivity in Colorectal Cancer by Regulating miR-296-5p/MSI1 Axis

Li¹,

Jiang²,

Zou³

et al. 2021

CMAR

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Background Exosomal circular RNAs (circRNAs) can act as biomarkers and play crucial roles in colorectal cancer (CRC) and radiosensitivity. The aim of this study was to explore the functions and regulatory mechanism of exosomal circRNA intraflagellar transport 80 (circ_IFT80) in tumorigenesis and radiosensitivity of CRC. Methods Exosomes were detected using transmission electron microscopy (TEM). Protein levels were determined by Western blot assay. The expression of circ_IFT80, microRNA-296-5p (miR-296-5p) and musashi1 (MSI1) was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cell cycle distribution, cell apoptosis, and cell proliferation were detected by flow cytometry and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, respectively. Colony formation assay was used to determine the radiosensitivity of cells. The interaction between miR-296-5p and circ_IFT80 or MSI1 was verified by dual-luciferase reporter assay. A xenograft tumor model was established to explore the role of exosomal circ_IFT80 in vivo. Results Circ_IFT80 was upregulated in exosomes derived from CRC patient serum and CRC cells. Exosomal circ_IFT80 or circ_IFT80 overexpression facilitated tumorigenesis by increasing cell proliferation and reducing apoptosis, and inhibited radiosensitivity via promoting colony formation and inhibiting apoptosis. Additionally, circ_IFT80 acted as a sponge of miR-296-5p, and miR-296-5p reversed the effects of circ_IFT80 on tumorigenesis and radiosensitivity. Moreover, MSI1 was a direct target of miR-296-5p. Furthermore, miR-296-5p overexpression inhibited tumorigenesis and promoted radiosensitivity by downregulating MSI1. Exosomal circ_IFT80 also accelerated tumor growth in vivo. Conclusion Exosomal circ_IFT80 promoted tumorigenesis and reduced radiosensitivity by regulating miR-296-5p/MSI1 axis, which might provide a novel avenue for treatment of CRC.

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“… 23 And Shang et al proposed that miR-142-3p also participated in the positive regulation of exosomal circPACRGL on colorectal cancer progression. 24 In addition, miR-142-3p had been found to have regulatory effects on chemotherapy sensitivity and cell autophagy in breast cancer. 25 In NSCLC, the previous studies revealed that miR-142-3p could hinder cancer progression and promote cancer chemosensitivity.…”

Section: Discussionmentioning

confidence: 99%

Circ_0020123 Increases ZFX Expression to Facilitate Non-Small Cell Lung Cancer Progression by Sponging miR-142-3p

Lu¹,

Lin³

et al. 2021

CMAR

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Background Circular RNA (circRNA) is involved in the progression of various cancers and has been shown to be an important potential target for cancer therapy. Circ_0020123 has been found to act as oncogene to participate in the malignant progression of non-small cell lung cancer (NSCLC). Therefore, exploring new mechanisms of circ_0020123 regulating NSCLC progression will help us better understand its role in NSCLC. Methods Relative expression levels of circ_0020123, microRNA (miR)-142-3p, and zinc-finger protein X-linked (ZFX) in tissues and cells were determined by quantitative real-time PCR (qRT-PCR). Cell proliferation, apoptosis, migration and invasion were assessed using cell counting kit 8 (CCK8) assay, colony formation assay, flow cytometry and transwell assay. Western blot (WB) analysis was used to detect relative protein level. Besides, the interaction between miR-142-3p and circ_0020123 or ZFX was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Results Our results showed that circ_0020123 was upregulated in NSCLC, and its knockdown could suppress NSCLC cell proliferation, migration, invasion, and promote apoptosis. Circ_0020123 was found to interact with miR-142-3p. The inhibition effect of circ_0020123 silencing on NSCLC progression could be reversed by miR-142-3p inhibitor. ZFX could be targeted by miR-142-3p. The silencing of ZFX could hinder the progression of NSCLC and abolish the promotion effect of miR-142-3p inhibitor on NSCLC progression. In addition, circ_0020123 silencing inhibited NSCLC tumorigenesis by the miR-142-3p/ZFX axis. Conclusion These findings suggested that circ_0020123 might be a potential therapy target for NSCLC, which could promote NSCLC progression through regulating the miR-142-3p/ZFX axis.

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Exosomal circPACRGL promotes progression of colorectal cancer via the miR-142-3p/miR-506-3p- TGF-β1 axis

Cited by 326 publications

References 27 publications

Exosomal circRNAs: Sorting Mechanisms, Roles and Clinical Applications in Tumors

Exosomal circRNAs: Sorting Mechanisms, Roles and Clinical Applications in Tumors

Exosomal circ_IFT80 Enhances Tumorigenesis and Suppresses Radiosensitivity in Colorectal Cancer by Regulating miR-296-5p/MSI1 Axis

Circ_0020123 Increases ZFX Expression to Facilitate Non-Small Cell Lung Cancer Progression by Sponging miR-142-3p

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