Exosomal microRNA-205 is involved in proliferation, migration, invasion, and apoptosis of ovarian cancer cells via regulating VEGFA

Abstract

BackgroundRecently, the impact of microRNAs (miRNAs) and exosome on ovarian cancer has been assessed in many studies. We aim to explore the mechanism of exosomes transferring miR-205 in ovarian cancer, and confirm its diagnostic value in ovarian cancer.MethodsThe expression of miR-205 of ovarian cancer patients and healthy people was detected by RT-qPCR, and the diagnostic value of miR-205 was evaluated. The exosomes derived from SKOV3 cells were identified. Ovarian cancer SKOV3 donor cells and receptor cells were used to measure the proliferation, migration, invasion, apoptosis and cell cycle by a series of experiments. The binding site between miR-205 and vascular endothelial growth factor A (VEGFA) was evaluated by bioinformatics tool and dual-luciferase reporter gene assay.ResultsMiR-205 was up-regulated in ovarian cancer, and up-regulated miR-205 could enhance the risk of ovarian cancer and was one of its risk factors. After SKOV3 cells-derived exosomes were transiently introduced with miR-205 mimics, the cell proliferation, migration and invasion in ovarian cancer were elevated, the apoptosis of ovarian cancer cells was attenuated, and the epithelial–mesenchymal transition (EMT) protein E-cadherin was down-regulated, while Vimentin was elevated. VEGFA was identified to be a target gene of miR-205.ConclusionThis study suggests that exosomes from donor ovarian cancer cell SKOV3 shuttled miR-205 could participate in the regulation of the proliferation, migration, invasion, apoptosis as well as EMT progression of receptor SKOV3 cells by targeting VEGFA.

Ligustrazine inhibits the proliferation and migration of ovarian cancer cells via regulating miR-211

“…EMT is considered an important cellular event during the progression of tumor metastasis [ 20 , 23 ]. It has been reported that LSZ could inhibit EMT in different types of cancer cells.…”

“…Unlike message RNAs (mRNA), miRNAs are not translated into a protein, although they can bind to the 3'-UTR of their target mRNAs and can epigenetically inhibit the expression of their target genes (17,18). Over the past few decades, the roles of miRNAs in different types of cancers, including OC, have been extensively investigated (19,20), and several miRNAs have been identified as potential therapeutic targets for the treatment of OC (21)(22)(23)(24).…”

microRNA-214 Prevents Traits of Cutaneous Squamous Cell Carcinoma via VEGFA and Bcl-2

“… 20 Furthermore, VEGFA was identified as a putative target of miR-205 in ovarian cancer and colorectal cancer, whereas VEGFA silencing markedly diminished colorectal cancer cell viability, migration and invasion. 21 , 22 Most importantly, miR-652-5p targeted VEGFA to suppress esophageal squamous cell carcinoma cell proliferation and metastasis. 23 On the other hand, miR-365 was found to compromised the migration and invasion, whereas enhanced apoptosis in melanoma cell lines, which was at least partially due to downregulation of the oncogene Bcl-2.…”

microRNA-214 prevents traits of cutaneous squamous cell carcinoma via VEGFA and Bcl-2

“…In non-small cell lung cancer, VEGFA silencing reversed the stimulative role of circ0021205 in cell proliferative, migratory, and invasive abilities [18]. Furthermore, VEGFA was identi ed as a putative target of miR-205 in ovarian cancer and colorectal cancer, whereas VEGFA silencing markedly diminished colorectal cancer cell viability, migration and invasion [19,20]. Most importantly, miR-652-5p targeted VEGFA to suppress esophageal squamous cell carcinoma cell proliferation and metastasis [21].…”