Exosomes in Pancreatic Cancer: from Early Detection to Treatment

Armstrong, Emily A.; Beal, Eliza W.; Chakedis, Jeffery; Paredes, Anghela Z.; Moris, Demetrios; Pawlik, Timothy M.; Schmidt, Carl; Dillhoff, Mary

doi:10.1007/s11605-018-3693-1

Cited by 43 publications

(37 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several evidence suggest that exosomes analysis is beneficial for understanding the mechanisms associated with the development, metastasis, and drug resistance of PC. Meanwhile, numerous studies have reported about the possibility of using exosomal components as diagnosis biomarkers and therapeutic targets for PC . Exosomes contain a diverse array of functional biomolecules, including proteins, lipids, and nucleic acids, which are transferred from donor cells to recipient cells for modulating intracellular signaling and intercellular communication.…”

Section: Discussionmentioning

confidence: 99%

Characterization and proteomic profiling of pancreatic cancer‐derived serum exosomes

Jiao

Jin

Jiang

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

Pancreatic cancer (PC) is one of the most lethal cancers known worldwide, and its prognosis is poor in most patients. Exosomes are nanosized extracellular vesicles, which are released from various cell types. They are involved in cellular communication. The diagnosis and treatment of PC were improved substantially with exosomes. In this study, we isolated PC-derived exosomes and investigated their proteomic profile. Then, we conducted bioinformatic analysis on proteomic data. Differential ultracentrifugation was performed to isolate exosomes from human serum samples and four PC cell lines. Transmission electron microscopy and Western blot analysis were used to characterize the isolated exosomes. Liquid chromatography coupled with tandem mass spectrometry was conducted to identify the proteome of serum exosomes. Proteomic analysis demonstrated that all the serum exosomes were derived from three cohorts of human subjects; these serum exosomes contained a total of 655 proteins, out of which 315 proteins overlapped with ExoCarta database. Gene oncology and kyoto encyclopedia of genes and genomes analyses provided the functional annotation of the proteome. Interestingly, 18 or 14 proteins were upregulated and 11 or 14 proteins were downregulated in serum exosomes derived from patients with PC as compared with in serum exosomes derived from healthy volunteers or from pancreatitis patients respectively. Annexin A11, a calcium-dependent phospholipid-binding protein, was expressed in a PC cell line (CFPAC-1)-derived exosomes and in tumor tissues of patients with PC, respectively. Our data provided a basic foundation for further studies on the protein composition of PC-derived exosomes and its involvement in PC biology.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization and proteomic profiling of pancreatic cancer‐derived serum exosomes

Jiao

Jin

Jiang

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…Exosomes also participate in numerous important physiological and pathological processes, including intercellular communication, cell motility, angiogenesis ( 13 ), immune response ( 14 ), tumor development and metastasis ( 15 , 16 ). Owing to their bioactive cargo, exosomes may serve as messengers and may offer valuable information for the diagnosis and prognosis of disease ( 17 ).…”

Section: Introductionmentioning

confidence: 99%

A highly efficient method for isolating urinary exosomes

Zhu

Wang

et al. 2018

Int J Mol Med

View full text Add to dashboard Cite

In the present study, a highly efficient method, referred to as optimized ultrafiltration (OUF), was developed. This method is effective for exosome purification and also facilitates clinical work involving substantial urinary exosome isolation. In the OUF method, 0.22-µm filters along with a dialysis membrane with a molecular weight cut-off of 10,000 kDa were introduced, in order to remove extracellular microvesicles that were >200 nm and concentrate the supernatant up to 1/50 of the initial volume. The existence, purity and production of the exosomes isolated by OUF and conventional ultracentrifugation (UC) were systematically compared by transmission electron microscopy, western blotting and nanoparticle tracking analysis. In addition, colloidal Coomassie-stained gel and reverse transcription-quantitative polymerase chain reaction were used to investigate the stability and integrity of exosomes isolated by these two protocols. The time required and cost of these two methods in the process of isolating urinary exosomes were also estimated. The results indicated that OUF clearly outperforms UC in quantity, quality and biological stability, and this improved method may have extensive applications in the growing fields of clinical biomarker discovery and exosome research.

show abstract

“…In contrast, more promising results are obtained in solid tumors including those of prostate, lung, esophagus and ovary [121][122][123][124][125]. In particular, using EVs for early detection of pancreatic cancer have been studied extensively [126][127][128][129].…”

Section: Diagnostic/prognostic Potential Of Ev Biomarkersmentioning

confidence: 99%

The potential diagnostic and prognostic role of extracellular vesicles in glioma: current status and future perspectives

et al. 2019

View full text Add to dashboard Cite

Lack of appropriate diagnostic/prognostic tools for glioblastoma (GB) is considered one of the major setbacks in the early diagnosis and treatment of this deadly brain tumor. The current gold standard for its diagnosis and staging still relies on invasive biopsy followed by histological examination as well as molecular profiling. Nevertheless, noninvasive approaches are being explored and one example is through the investigation of extracellular vesicles (EVs) in the biofluids of GB patients. EVs are known to carry molecular cargoes such as DNA, mRNA, miRNA, proteins and lipids in almost every type of body fluids. Thus, molecular signature of GB may be present in the EVs derived from these patients. This review focuses on the diagnostic/prognostic potential of EVs in GB, through presenting recent studies on (i) molecular components of EVs, (ii) links between EVs and GB tumor microenvironment, and (iii) clinical potential of EV biomarkers, together with the technical shortcomings researchers need to consider for future studies. ARTICLE HISTORYAccording to their origin and/or size, EVs can be classified into three subclasses, which are apoptotic bodies,

show abstract

Exosomes in Pancreatic Cancer: from Early Detection to Treatment

Cited by 43 publications

References 101 publications

Characterization and proteomic profiling of pancreatic cancer‐derived serum exosomes

Characterization and proteomic profiling of pancreatic cancer‐derived serum exosomes

A highly efficient method for isolating urinary exosomes

The potential diagnostic and prognostic role of extracellular vesicles in glioma: current status and future perspectives

Contact Info

Product

Resources

About