Exosomes Play an Important Role in the Progression of Plasma Cell Mastitis via the PI3K-Akt-mTOR Signaling Pathway

Wang, Xiaohong; Han, Yang; Liu, Jian; Zhang, Yingze; Chen, Kai; Guo, Jiwei; Guo, Qingqun; Song, Lei; Sun, Hongwei; Hua, Yingqi; Zhang, Guoqiang; Xu, Shujian; Guo, Fei; Yang, Zhenlin

doi:10.1155/2019/4312016

Cited by 13 publications

(14 citation statements)

References 27 publications

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“…RNA-SEQ was used to analyze the difference in exosome transcriptome expression between PCM and normal breast tissue. It was found that p-Akt was produced too much in the process of PCM, and inhibiting exosome secretion could significantly inhibit inflammatory cell infiltration, so that the breast duct in the PCM mouse model could maintain a good structure [ 60 ]. Overall, the above pathways may be closely relevant to GLNBD treatment of PCM.…”

Section: Discussionmentioning

confidence: 99%

Study the Mechanism of Gualou Niubang Decoction in Treating Plasma Cell Mastitis Based on Network Pharmacology and Molecular Docking

Yang

2022

BioMed Research International

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Objective. Explore the potential molecular mechanisms behind the therapeutic functions of Gualou Niubang decoction (GLNBD) in the treatment of plasma cell mastitis (PCM) by network pharmacology and molecular docking. Methods. GLNBD is a formula of Chinese traditional medicine consisting of 12 herbs. The potential active ingredients of GLNBD and their target genes were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform database, and PCM-related target genes were obtained from GeneCards, OMIM, and NCBI databases, using R language to obtain intersection targets; then, the STRING database and Cytoscape software were used to establish protein-protein interaction networks and herb ingredient target networks. DAVID was used to perform GO and KEGG pathway enrichment analyses on the intersection target. PyMoL-2.5.0 and AutoDock Tools-1.5.6 were used to verify the molecular docking. Results. 164 ingredients and 58 intersection targets were obtained in the treatment of PCM by GLNBD. Four key active compounds and four key proteins were identified. Then, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that biological functions of potential target genes were associated with negative regulation of the apoptotic process, response to hypoxia, positive regulation of transcription, and DNA-templated, with related pathways involving the pathway in cancer, phosphatidylinositol 3-kinase (PI3K) Akt signaling pathway, and AGE-RAGE signaling pathway in diabetic complications. Moreover, the binding activities of key target genes and essential active compounds of Chinese herbal medicines in GLNBD were further validated by molecular docking. The results showed that the docking results were stable and had good binding ability. Conclusion. This study suggested that four potential key active components, including quercetin, luteolin, fisetin, and kaempferol, were identified in GLNBD, which could interact with ALB, EGFR, IL-6, and VEGFA modulating the activation of the pathway in cancer, PI3K-Akt pathway, and AGE-RAGE signaling pathway in diabetic complications.

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Section: Discussionmentioning

confidence: 99%

Study the Mechanism of Gualou Niubang Decoction in Treating Plasma Cell Mastitis Based on Network Pharmacology and Molecular Docking

Yang

2022

BioMed Research International

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“…The function of the exosome has been established in diseases such as cancer, diabetes, and inflammation (Kulshreshtha et al, 2013). Wang et al (2019) found that mice treated with GW 4869, which inhibits the secretion of exosomes, illustrated a significantly reduced inflammatory cell infiltration. Here, the exosomes extracted from rat serum were characterized using ultracentrifugation by TEM, NTA, and specific protein markers.…”

Section: Discussionmentioning

confidence: 99%

Serum‐derived exosomes from SD rats induce inflammation in macrophages through the mTOR pathway

Wang

Liu

Sun

et al. 2022

J of Applied Toxicology

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Inhalation of beryllium and its compounds can cause lung injuries, resulting from inflammation and oxidative stress. Multivesicular bodies (MVB), such as exosomes, are membrane vesicles produced by early and late endosomes that mediate intercellular communications. However, the role of exosomes in beryllium toxicity has not been elucidated. This current study aimed to investigate the functional role of exosomes in lung injury resulting from beryllium sulfate (BeSO 4 ). Here, Sprague-Dawley (SD) rats were exposed to 4, 8, and 12 mg/kg BeSO 4 by nonexposed intratracheal instillation. Murine macrophage (RAW 264.7) cells were pretreated with 50 nmol/L rapamycin (an mTOR signaling pathway inhibitor) for 30 min and then cultured for 24 h with 100 μg/mL exosomes, which had been previously isolated from the serum of 12 mg/kg BeSO 4 -treated SD rats. Compared with those of the controls, exposure to BeSO 4 in vivo increased LDH activity, elevated levels of inflammatory cytokines (IL-10, TNF-α, and IFN-γ) alongside inflammation-related proteins expression (COX-2 and iNOS), and enhanced secretion of exosomes from the SD rat's serum. Moreover, the BeSO 4 -Exos-induced upregulation of LDH activity and inflammatory responses in RAW 264.7 cells can be alleviated following pretreatment with rapamycin. Collectively, these results suggest that serum exosomes play an important role in pulmonary inflammation induced by BeSO 4 in RAW 264.7 cells via the mTOR pathway.

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“…Studies have shown that ICAM-1 in mammary duct epithelium and endothelial cells play an important role in PCM inflammation. [ 11 ] However, at present, scholars have not reached a consensus on the etiology of PCM, most of the debates focus on immune, [ 12 ] nipple congenital malformation, and so on, such as Liu et al show that IL-6/STAT3 signaling pathway is activated in PCM, and it is speculated that it plays an important role in the pathogenesis of PCM. For treatment, western medicine advocates early and complete resection of lesions and correction of nipple retraction is [ 13 ] effective treatment.…”

Section: Discussionmentioning

confidence: 99%

Chinese herbal compound combined with western medicine therapy in the treatment of plasma cell mastitis

Zhang

Xu²,

Zhang³

et al. 2020

Medicine

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Background: Plasma cell mastitis (PCM) is a benign suppurative disease of the breast based on the expansion of mammary ducts and infiltration of plasma cells. It is relatively rare clinically, and its main manifestations include nonperiodic breast pain, nipple discharge, areola lump, nipple depression, nipple fistula, among others. Modern medicine is mainly surgical treatment, which is easy to recur. The clinical practice shows that the overall treatment of patients with TCM syndrome differentiation using oral medicine combined with western medicine therapy, combined internal and external treatment, can significantly improve the curative effect, prevent recurrence, has a certain therapeutic advantage, but lack of evidence of evidence-based medicine. The purpose of this study is to study the efficacy and safety of oral traditional Chinese medicine (TCM) combined with western medicine therapy in the treatment of PCM. Methods: Use computer to retrieve English databases (PubMed, Embase, Web of Science, the Cochrane Library) and Chinese databases (CNKI, Wan Fang, VIP, Chinese biomedical database), from the establishment of database to September 2020, for randomized controlled trials(RCTs) of oral TCM combined with western medicine therapy in the treatment of PCM, two researchers independently extracted the data and evaluated the quality of the included research, and meta-analysis was conducted on the included literatures using RevMan5.3 software. Results: This study evaluated the efficacy and safety of oral TCM combined with western medicine therapy in the treatment of PCM from the aspects of effective rate, symptom score, recurrence rate, adverse reaction rate, and patient satisfaction. Conclusion: This study will provide reliable evidence-based evidence for the clinical application of oral TCM combined with western medicine therapy in the treatment of PCM. Ethics and dissemination: The purpose of this study is to sort out and analyze the literature. This systematic review also does not involve endangering participant rights. Ethical approval was not required. The results may be published in a peer-reviewed journal or disseminated at relevant conferences. OSF Registration number: doi 10.17605/OSF.IO/K9A78.

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Exosomes Play an Important Role in the Progression of Plasma Cell Mastitis via the PI3K-Akt-mTOR Signaling Pathway

Cited by 13 publications

References 27 publications

Study the Mechanism of Gualou Niubang Decoction in Treating Plasma Cell Mastitis Based on Network Pharmacology and Molecular Docking

Study the Mechanism of Gualou Niubang Decoction in Treating Plasma Cell Mastitis Based on Network Pharmacology and Molecular Docking

Serum‐derived exosomes from SD rats induce inflammation in macrophages through the mTOR pathway

Chinese herbal compound combined with western medicine therapy in the treatment of plasma cell mastitis

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