Exosomes: The New Mediator of Peritoneal Membrane Function

Yu, Manshu; Shi, Jun; Sheng, Maoyin

doi:10.1159/000490821

Cited by 9 publications

(6 citation statements)

References 113 publications

(86 reference statements)

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“…Exosomes are nano-sized extracellular vesicles originating from the endosomal pathway that exist in almost all body fluids and are promising source of biomarkers 28 . Exosomes carry a cargo of proteins, lipids and nucleic acids, that can participate in cell-to-cell signaling affecting nearby and distant cells 29 , playing a role in EMT and peritoneal fibrosis 30 , 31 , and in angiogenesis processes 32 .…”

Section: Introductionmentioning

confidence: 99%

Proteomic profile of mesothelial exosomes isolated from peritoneal dialysis effluent of children with focal segmental glomerulosclerosis

Bruschi

Porta

Panfoli

et al. 2021

Sci Rep

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Peritoneal dialysis (PD) is the worldwide recognized preferred dialysis treatment for children affected by end-stage kidney disease (ESKD). However, due to the unphysiological composition of PD fluids, the peritoneal membrane (PM) of these patients may undergo structural and functional alterations, which may cause fibrosis. Several factors may accelerate this process and primary kidney disease may have a causative role. In particular, patients affected by steroid resistant primary focal segmental glomerulosclerosis, a rare glomerular disease leading to nephrotic syndrome and ESKD, seem more prone to develop peritoneal fibrosis. The mechanism causing this predisposition is still unrecognized. To better define this condition, we carried out, for the first time, a new comprehensive comparative proteomic mass spectrometry analysis of mesothelial exosomes from peritoneal dialysis effluent (PDE) of 6 pediatric patients with focal segmental glomerular sclerosis (FSGS) versus 6 patients affected by other primary renal diseases (No FSGS). Our omic study demonstrated that, despite the high overlap in the protein milieu between the two study groups, machine learning allowed to identify a core list of 40 proteins, with ANXA13 as most promising potential biomarker, to distinguish, in our patient population, peritoneal dialysis effluent exosomes of FSGS from No FSGS patients (with 100% accuracy). Additionally, the Weight Gene Co-expression Network Analysis algorithm identified 17 proteins, with PTP4A1 as the most statistically significant biomarker associated to PD vintage and decreased PM function. Altogether, our data suggest that mesothelial cells of FSGS patients are more prone to activate a pro-fibrotic machinery. The role of the proposed biomarkers in the PM pathology deserves further investigation. Our results need further investigations in a larger population to corroborate these findings and investigate a possible increased risk of PM loss of function or development of encapsulating peritoneal sclerosis in FSGS patients, thus to eventually carry out changes in PD treatment and management or implement new solutions.

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Section: Introductionmentioning

confidence: 99%

Proteomic profile of mesothelial exosomes isolated from peritoneal dialysis effluent of children with focal segmental glomerulosclerosis

Bruschi

Porta

Panfoli

et al. 2021

Sci Rep

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“…8 Previous studies have reported that peritoneal cells respond to the dialysis exposure by releasing various EVs, which can be found in PD effluent. 9 These PD effluent-derived EV proteomes showed alteration earlier than peritoneal equilibration test monitoring, suggesting the potential of EV proteins as biomarkers of peritoneal function alteration for patients undergoing PD. 10 Moreover, it has been reported that specific molecular cargo in EVs, such as aquaporin 1 and glycoprotein 96, can be used to evaluate the status of peritoneum.…”

Section: ■ Introductionmentioning

confidence: 90%

“…Since the specific molecular composition of EVs reflects physiological or pathological state of parent cells, EVs isolated from body fluids are increasingly recognized as promising biomarkers for disease progression . Previous studies have reported that peritoneal cells respond to the dialysis exposure by releasing various EVs, which can be found in PD effluent . These PD effluent-derived EV proteomes showed alteration earlier than peritoneal equilibration test monitoring, suggesting the potential of EV proteins as biomarkers of peritoneal function alteration for patients undergoing PD .…”

Section: Introductionmentioning

confidence: 99%

Proteomic Characterization of Peritoneal Extracellular Vesicles in a Mouse Model of Peritoneal Fibrosis

Huang

Sun

et al. 2023

J. Proteome Res.

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Peritoneal fibrosis progression is regarded as a significant cause of the loss of peritoneal function, markedly limiting the application of peritoneal dialysis (PD). However, the pathogenesis of peritoneal fibrosis remains to be elucidated. Tissue-derived extracellular vesicles (EVs) change their molecular cargos to adapt the environment alteration, mediating intercellular communications and play a significant role in organ fibrosis. Hence, we performed, for the first time, four-dimensional label-free quantitative liquid chromatography–tandem mass spectrometry proteomic analyses on EVs from normal peritoneal tissues and PD-induced fibrotic peritoneum in mice. We demonstrated the alterations of EV concentration and protein composition between normal control and PD groups. A total of 2339 proteins containing 967 differentially expressed proteins were identified. Notably, upregulated proteins in PD EVs were enriched in processes including response to wounding and leukocyte migration, which participated in the development of fibrosis. In addition, EV proteins of the PD group exhibited unique metabolic signature compared with those of the control group. The glycolysis-related proteins increased in PD EVs, while oxidative phosphorylation and fatty acid metabolism-related proteins decreased. We also evaluated the effect of cell-type specificity on EV proteins, suggesting that mesothelial cells mainly cause the alterations in the molecular composition of EVs. Our study provided a useful resource for further validation of the key regulator or therapeutic target of peritoneal fibrosis.

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“…CXCL1 may be an important pro-angiogenic agent [ 67 ]. Catar et al showed that CXCL1 secreted by PMCs directly promotes human microvascular endothelial tube formation [ 67 ], and VEGF accelerates the PA process by participating in angiogenesis [ 68 , 69 ]. CXCL1 can also upregulate the expression of PAI-1 and promote fibrin deposition [ 3 ].…”

Section: The Mechanism Of Pmcs In Promoting Pasmentioning

confidence: 99%

Mechanisms of Peritoneal Mesothelial Cells in Peritoneal Adhesion

Wang

Guo

2022

Biomolecules

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A peritoneal adhesion (PA) is a fibrotic tissue connecting the abdominal or visceral organs to the peritoneum. The formation of PAs can induce a variety of clinical diseases. However, there is currently no effective strategy for the prevention and treatment of PAs. Damage to peritoneal mesothelial cells (PMCs) is believed to cause PAs by promoting inflammation, fibrin deposition, and fibrosis formation. In the early stages of PA formation, PMCs undergo mesothelial–mesenchymal transition and have the ability to produce an extracellular matrix. The PMCs may transdifferentiate into myofibroblasts and accelerate the formation of PAs. Therefore, the aim of this review was to understand the mechanism of action of PMCs in PAs, and to offer a theoretical foundation for the treatment and prevention of PAs.

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Exosomes: The New Mediator of Peritoneal Membrane Function

Cited by 9 publications

References 113 publications

Proteomic profile of mesothelial exosomes isolated from peritoneal dialysis effluent of children with focal segmental glomerulosclerosis

Proteomic profile of mesothelial exosomes isolated from peritoneal dialysis effluent of children with focal segmental glomerulosclerosis

Proteomic Characterization of Peritoneal Extracellular Vesicles in a Mouse Model of Peritoneal Fibrosis

Mechanisms of Peritoneal Mesothelial Cells in Peritoneal Adhesion

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