Expanded polytetrafluoroethylene as a substrate for retinal pigment epithelial cell growth and transplantation in age-related macular degeneration

Krishna, Yamini; Sheridan, Carl; Kent, David; Kearns, Victoria; Grierson, Ian; Williams, Rachel

doi:10.1136/bjo.2009.169953

Cited by 39 publications

(38 citation statements)

References 26 publications

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“…The primary cell isolation methods, characterisation of epithelial purity and culture conditions for both cell types have been described previously [4]. ARPE-19 were used at passage 18-20; hRPE were used at passage 8-12.…”

Section: Cell Culturementioning

confidence: 99%

“…However, it is hydrophobic and will not allow cell attachment. Ammonia gas plasma treatment of e-PTFE has been shown to improve its hydrophilicity significantly and promote the development of a monolayer of functional RPE cells in vitro [4]. Ammonia gas plasma treatment followed by a post treatment in PBS has been shown to cause defluorination of the surface and the incorporation of oxygen-containing functionalities [5,6].…”

Section: Introductionmentioning

confidence: 99%

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Plasma polymer coatings to aid retinal pigment epithelial growth for transplantation in the treatment of age related macular degeneration

Kearns

Mistry

Mason

et al. 2012

J Mater Sci: Mater Med

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Subretinal transplantation of functioning retinal pigment epithelial (RPE) cells grown on a synthetic substrate is a potential treatment for age-related macular degeneration (AMD), a common cause of irreversible vision loss in developed countries. Plasma polymers give the opportunity to tailor the surface chemistry of the artificial substrate whilst maintaining the bulk properties. In this study, plasma polymers with different functionalities were investigated in terms of their effect on RPE attachment and growth. Plasma polymers of acrylic acid (AC), allyl amine (AM) and allyl alcohol (AL) were fabricated and characterised using X-ray photoelectron spectroscopy (XPS) and water contact angle measurements. Octadiene (OD) hydrocarbon films and tissue culture polystyrene were used as controls. Wettability varied from hydrophobic OD to relatively hydrophilic AC. XPS demonstrated four very different surfaces with the expected functionalities. Attachment, proliferation and morphological examination of an RPE cell line and primary RPE cells were investigated. Both cell types grew on all surfaces, with the exception of OD, although the proliferation rate of primary cells was low. Good epithelial morphology was also demonstrated. Plasma polymerised films show potential as cell carrier surfaces for RPE cells in the treatment of AMD.

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Section: Cell Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Plasma polymer coatings to aid retinal pigment epithelial growth for transplantation in the treatment of age related macular degeneration

Kearns

Mistry

Mason

et al. 2012

J Mater Sci: Mater Med

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“…To be able to do this it is necessary to modify the surface of the ePTFE to overcome its hydrophobicity using an ammonia gas plasma. [53] This renders the surface hydrophilic without influencing the structural or mechanical properties. We found that we could get good cell growth of primary conjunctival cells on the plasmatreated ePTFE by 14 d and that about 15% of the cells stained positive for MUC5AC although after longer time periods in vitro the total cell number reduced.…”

Section: Conjunctiva Damage and Repairmentioning

confidence: 99%

Biomaterials for Regenerative Medicine Approaches for the Anterior Segment of the Eye

Williams

Lace

Kennedy

et al. 2018

Adv Healthcare Materials

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The role of biomaterials in tissue engineering and regenerative medicine strategies to treat vision loss associated with damage to tissues in the anterior segment of the eye has been studied for several years. This has mostly involved replacement and support for the cornea and conjunctiva. These are complex tissues with specific functional requirements for different parts of the tissue. Amniotic membrane (AM) is used in clinical practice to transplant autologous or allogenic cells to the corneal surface. Fibrin gels have also progressed to clinical use under specific conditions. Alternatives to AM such as collagen gels, other natural materials, for example keratin and silks, and synthetic polymers have received considerable attention in laboratory and animal studies. This experience is building a body of evidence to demonstrate the potential of tissue engineering and regenerative medicine in corneal and conjunctival reconstruction and can also lead to other applications in the anterior segment of the eye, for example, the trabecular meshwork. There is a real clinical need for new procedures to overcome vision loss but there are also opportunities for developments in ocular applications to lead to biomaterials innovations for use in other clinical areas.

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“…The rods provide trophic support to cone cells, and, consequently, healthy rod cell transplantation into RP mice lacking rods can delay cone death (8). Providing physical support, for example with the help of biopolymers, may be important for both cone viability and function (19). For end-stage retinal degeneration, in which the residual cone cells are nonfunctional or are lost, strategies such as transplanting photoreceptors (20), implanting electronic prostheses (21), or using optogenetic strategies (22) may be beneficial for restoring navigational vision.…”

Section: An "Aye" For Nrf2mentioning

confidence: 99%

Exploiting metabolic and antioxidant pathways to maintain vision in blinding disease

Ramachandran¹,

Song²,

Bennett³

2015

J. Clin. Invest.

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Expanded polytetrafluoroethylene as a substrate for retinal pigment epithelial cell growth and transplantation in age-related macular degeneration

Abstract: ePTFE can be surface-modified to support an intact functional monolayer of healthy RPE cells with normal morphology and the ability to perform RPE-specific functions. Following further investigation ePTFE may be considered for use in transplantation.

Cited by 39 publications

References 26 publications

Plasma polymer coatings to aid retinal pigment epithelial growth for transplantation in the treatment of age related macular degeneration

Plasma polymer coatings to aid retinal pigment epithelial growth for transplantation in the treatment of age related macular degeneration

Biomaterials for Regenerative Medicine Approaches for the Anterior Segment of the Eye

Exploiting metabolic and antioxidant pathways to maintain vision in blinding disease

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