Expanding the host range: infection of a reptilian host (Furcifer pardalis) by an atypical Brucella strain

Eisenberg, Tobias; Schlez, Karen; Fawzy, Ahmad; Völker, I.; Hechinger, Silke; Curić, Mersiha; Schauerte, Nicole; Geiger, Christina; Blom, Jochen; Scholz, Holger C.

doi:10.1007/s10482-020-01448-9

Cited by 16 publications

(11 citation statements)

References 24 publications

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“…2020), reptiles (Eisenberg et al. 2020), and a saltwater fish (Eisenberg et al. 2017), extend the host range of the infection, suggesting that the epidemiology of brucellosis may be even more complex in the aquatic environment.…”

Section: Discussionmentioning

confidence: 99%

“…Several aspects of the epidemiology of infection in the marine environment remain to be elucidated, including the identification of the reservoirs of several strains of B. ceti and B. pinnipedialis and the role of lung nematodes in infection transmission (Rhyan et al 2018). The recent reports of natural Brucella infections in cold-blooded animals, including amphibians (Jaÿ et al 2020), reptiles (Eisenberg et al 2020), and a saltwater fish (Eisenberg et al 2017), extend the host range of the infection, suggesting that the epidemiology of brucellosis may be even more complex in the aquatic environment.…”

Section: Discussionmentioning

confidence: 99%

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Brucella Infection Investigation in Cetaceans and Manatees in Northeast Brazil

et al. 2021

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Among the bacterial infections that impair the health status of marine mammals, those caused by Brucella spp. are the most reported worldwide. Brucella infections in marine mammals can result in acute or chronic disease and are associated with variable clinical outcomes, depending on the organ involved during the infectious process, infection route, host immunity, and strain pathogenicity. Asymptomatic infections may also occur. The current study expands the investigation of Brucella infection in northeast Brazil by analyzing 19 dead, stranded cetaceans and 52 Antillean manatees Trichechus manatus manatus. The manatees included 8 dead, captive manatees and 44 live specimens, of which 10 were analyzed only after reintroduction into the wild as part of a rehabilitation program, 9 were analyzed both while in captivity or semi‐captivity and after reintroduction, 20 were sampled only in captivity or semi‐captivity, and 5 were free‐living manatees. Serological tests were used to screen for antibodies against smooth Brucella spp. Whole blood, swabs, and tissue samples were screened for Brucella spp. DNA by PCR. Samples with positive PCR results were cultured for Brucella spp. isolation. All manatees yielded negative results in serological and molecular tests. Brucella spp. DNA was detected in the kidney of one adult Guiana dolphin Sotalia guianensis exhibiting necrosis in the liver. No growth of Brucella spp. was observed via microbiological culturing. This study is the first report of Brucella spp. DNA detection in cetaceans in the state of Pernambuco, and it highlights the importance of conducting systematic monitoring for the presence of Brucella infection in marine mammals along the Brazilian coast, especially in the northeast region, where several cases have been reported.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Brucella Infection Investigation in Cetaceans and Manatees in Northeast Brazil

et al. 2021

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“…Other species genetically related to an atypical group within the genus have been described in different host species: Brucella microti -like in marsh frogs ( Pelophylax ridibundus ) [ 11 ]; Brucella vulpis in the red fox ( V. vulpes ) [ 13 ]; Brucella inopinata in White’s and Denny’s tree frogs, ( Ranoidea caerulea and Zhangixalus dennysi ) [ 169 ] and humans; Brucella papionis in baboons ( Papio spp.) [ 15 ], without mentioning Brucella strains isolates from lungworms in porpoise ( Phocoena phocoena ) [ 170 ], blue-spotted ribbontail ray ( Taeniura lymma ) [ 171 ] and reptile panther chameleon ( Furcifer pardalis ) [ 172 ].…”

Section: Reservoirs In Wildlifementioning

confidence: 99%

Brucella: Reservoirs and Niches in Animals and Humans

et al. 2021

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Brucella is an intracellular bacterium that causes abortion, reproduction failure in livestock and leads to a debilitating flu-like illness with serious chronic complications if untreated in humans. As a successful intracellular pathogen, Brucella has developed strategies to avoid recognition by the immune system of the host and promote its survival and replication. In vivo, Brucellae reside mostly within phagocytes and other cells including trophoblasts, where they establish a preferred replicative niche inside the endoplasmic reticulum. This process is central as it gives Brucella the ability to maintain replicating-surviving cycles for long periods of time, even at low bacterial numbers, in its cellular niches. In this review, we propose that Brucella takes advantage of the environment provided by the cellular niches in which it resides to generate reservoirs and disseminate to other organs. We will discuss how the favored cellular niches for Brucella infection in the host give rise to anatomical reservoirs that may lead to chronic infections or persistence in asymptomatic subjects, and which may be considered as a threat for further contamination. A special emphasis will be put on bone marrow, lymph nodes, reproductive and for the first time adipose tissues, as well as wildlife reservoirs.

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“…Brucella is a Gram-negative intracellular pathogen that causes brucellosis [1], it usually can be divided into 12 species in nature, including six so-called classic Brucella species, namely B. melitensis, B. abortus, B. suis, B. canis, B. ovis and B. neotomae, and six newly discovered Brucella species from wild mammals, amphibians and fish, namely B. microti, B. pinnipidialis, B. ceti, B. inopinata, B. papionis and B. vulpis. In the genus Brucella, B. melitensis, B. abortus and B. suis have good clinical significance [2][3][4]. Brucellosis in animals manifests itself in miscarriages and reduced fertility and is transmitted to humans by inhaling aerosolised bacteria or ingesting contaminated derivatives.…”

Section: Introductionmentioning

confidence: 99%

“…, and B. suis have good clinical significance [2][3][4]. Brucellosis in animals manifests itself in miscarriages and reduced fertility and is transmitted to humans by inhaling aerosolized bacteria or ingesting contaminated derivatives.…”

Section: Introductionmentioning

confidence: 99%

Design of a new multi-epitope vaccine against Brucella based on T and B cell epitopes using bioinformatics methods

et al. 2021

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Brucellosis is one of the most serious and widespread zoonotic diseases, which seriously threatens human health and the national economy. This study was based on the T/B dominant epitopes of Brucella outer membrane protein 22 (Omp22), outer membrane protein 19 (Omp19) and outer membrane protein 28 (Omp28), with bioinformatics methods to design a safe and effective multi-epitope vaccine. The amino acid sequences of the proteins were found in the National Center for Biotechnology Information (NCBI) database, and the signal peptides were predicted by the SignaIP-5.0 server. The surface accessibility and hydrophilic regions of proteins were analysed with the ProtScale software and the tertiary structure model of the proteins predicted by I-TASSER software and labelled with the UCSF Chimera software. The software COBEpro, SVMTriP and BepiPred were used to predict B cell epitopes of the proteins. SYFPEITHI, RANKpep and IEDB were employed to predict T cell epitopes of the proteins. The T/B dominant epitopes of three proteins were combined with HEYGAALEREAG and GGGS linkers, and carriers sequences linked to the N-and C-terminus of the vaccine construct with the help of EAAAK linkers. Finally, the tertiary structure and physical and chemical properties of the multi-epitope vaccine construct were analysed. The allergenicity, antigenicity and solubility of the multi-epitope vaccine construct were 7.37-11.30, 0.788 and 0.866, respectively. The Ramachandran diagram of the mock vaccine construct showed 96.0% residues within the favoured and allowed range. Collectively, our results showed that this multi-epitope vaccine construct has a high-quality structure and suitable characteristics, which may provide a theoretical basis for future laboratory experiments.

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Expanding the host range: infection of a reptilian host (Furcifer pardalis) by an atypical Brucella strain

Cited by 16 publications

References 24 publications

Brucella Infection Investigation in Cetaceans and Manatees in Northeast Brazil

Brucella Infection Investigation in Cetaceans and Manatees in Northeast Brazil

Brucella: Reservoirs and Niches in Animals and Humans

Design of a new multi-epitope vaccine against Brucella based on T and B cell epitopes using bioinformatics methods

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