Expansion and evolution of insect GMC oxidoreductases

Iida, Kaori; Cox-Foster, Diana; Yang, Xiaolong; Ko, Wen Ya; Cavener, Douglas R.

doi:10.1186/1471-2148-7-75

Cited by 59 publications

(67 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite comprehensive GMC gene analyses, phylogenetic relations of both clades have not yet been completely resolved [24,25]. But irrespective of whether GMCi and beetle GMCs cluster separately in two subfamilies [24] or have an intertwined evolutionary history [25], the corresponding T. castaneum GMCs are not close relatives.…”

Section: Discussionmentioning

confidence: 99%

“…Like other GMC oxidoreductase proteins, Pc8HGO and Pc8HGO-like possess the N-terminal b-a-b dinucleotide-binding motif (GxGxxG(x) 18 E) necessary to bind the flavin adenine dinucleotide cofactor [22,31,32]. Additionally, there are several blocks of conserved amino acid sequences common among GMC oxidoreductases (electronic supplementary material, figure S3) [24].…”

Section: Resultsmentioning

confidence: 99%

“…But irrespective of whether GMCi and beetle GMCs cluster separately in two subfamilies [24] or have an intertwined evolutionary history [25], the corresponding T. castaneum GMCs are not close relatives. Thus, both analyses support our findings that chrysomeline SAO and 8HGO have independent origins, as do their closely related T. castaneum counterparts (TcasGMCi5 and TcasGMCXM967481 also do not cluster).…”

Section: Discussionmentioning

confidence: 99%

“…As it has been shown earlier, SAOs and related sequences cluster in an insect GMCi clade closest to Tribolium castaneum GMCi5 [16,21]. By contrast, the Pc8HGO is affiliated with GMC oxidoreductases from T. castaneum (XM961538, XM961446, XM967481); according to a global insect GMC analysis [24], these cluster separately from their GMCi counterparts in the so-called beetle GMC clade. The origin of Pc8HGO within the beetle GMC clade is indicated by a close relationship to the T. castaneum GMC (XM967481) and is supported by high posterior probability and bootstrap values (1,97,92).…”

Section: (D) Catalytic Activity Of the Purified Pc8hgomentioning

confidence: 98%

“…These SAOs belong to the glucose-methanol-choline (GMC) oxidoreductase multi-gene family [22,23] and convert salicyl alcohol into salicylaldehyde [20]. Comparative genome analyses show that the GMC oxidoreductase family harbours genes, most of which are in a cluster and unique with respect to their expansion in insects [24][25][26]. Phylogenetic analyses of the functional SAOs from salicin-sequestering Chrysomelina sensu stricto strongly support the SAOs' common ancestry in the GMCi clade [16] irrespective of the evolutionary affiliation of the corresponding beetle species [5].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Independently recruited oxidases from the glucose-methanol-choline oxidoreductase family enabled chemical defences in leaf beetle larvae (subtribe Chrysomelina) to evolve

et al. 2014

View full text Add to dashboard Cite

Larvae of the leaf beetle subtribe Chrysomelina sensu stricto repel their enemies by displaying glandular secretions that contain defensive compounds. These repellents can be produced either de novo (iridoids) or by using plant-derived precursors (e.g. salicylaldehyde). The autonomous production of iridoids, as in Phaedon cochleariae, is the ancestral chrysomeline chemical defence and predates the evolution of salicylaldehyde-based defence. Both biosynthesis strategies include an oxidative step of an alcohol intermediate. In salicylaldehyde-producing species, this step is catalysed by salicyl alcohol oxidases (SAOs) of the glucose-methanol-choline (GMC) oxidoreductase superfamily, but the enzyme oxidizing the iridoid precursor is unknown. Here, we show by in vitro as well as in vivo experiments that P. cochleariae also uses an oxidase from the GMC superfamily for defensive purposes. However, our phylogenetic analysis of chrysomeline GMC oxidoreductases revealed that the oxidase of the iridoid pathway originated from a GMC clade different from that of the SAOs. Thus, the evolution of a host-independent chemical defence followed by a shift to a host-dependent chemical defence in chrysomeline beetles coincided with the utilization of genes from different GMC subfamilies. These findings illustrate the importance of the GMC multi-gene family for adaptive processes in plant-insect interactions.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: (D) Catalytic Activity Of the Purified Pc8hgomentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Independently recruited oxidases from the glucose-methanol-choline oxidoreductase family enabled chemical defences in leaf beetle larvae (subtribe Chrysomelina) to evolve

et al. 2014

View full text Add to dashboard Cite

show abstract

CHARACTERIZATION OF GLUCOSE‐INDUCED GLUCOSE OXIDASE GENE AND PROTEIN EXPRESSION IN Helicoverpa armigera LARVAE

Tang

Kang

et al. 2012

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Caterpillar labial salivary enzyme glucose oxidase (GOX) plays an important role in plant-insect interactions by suppressing the caterpillar-induced nicotine production in tobacco plants. In the present study, we cloned and characterized the GOX gene (HaGox) from labial salivary glands of Helicoverpa armigera larvae using 3' and 5' RACE, homologous analysis, phylogenetic analysis, LC-MS/MS, and qRT-PCR. The deduced GOX amino acid sequence (606 amino acids) shares 99% and 76% identity with H. zea and Spodoptera exigua GOX, respectively, and is consistent with the GOX protein sequence identified from H. armigera labial salivary gland. These results confirmed that the HaGox encode the GOX protein. Transcript levels of HaGox in larval labial salivary glands were significantly higher than those in midgut and hemolymph, respectively, and those of caterpillars reared on tobacco leaves coated with 0.1%, 1%, and 10% glucose solutions were significantly higher than those reared on 0.01% glucose-coated leaves or control tobacco leaves. Western blot and native PAGE showed that GOX protein expression levels and GOX enzymatic activities also increased with dietary glucose. These results proved that GOX expressions in larval labial salivary glands induced by dietary glucose were exhibited not only on transcriptional levels, but also on translational/posttranslational levels.

show abstract