Expansion of blood IgG 4 + B, T H 2, and regulatory T cells in patients with IgG 4 -related disease

Heeringa, Jorn J.; Karim, Faiz; Laar, Jan A. M. van; Verdijk, Robert M.; Paridaens, Dion; Hagen, P. Martin van; Zelm, Menno C. van

doi:10.1016/j.jaci.2017.07.024

Cited by 71 publications

(61 citation statements)

References 75 publications

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“…Next, we studied whether Th subsets were affected by treatment. Th1, Th2 and Th17 cells were defined within the CD45RA‐ memory compartment based on differential expression of the chemokine receptors CCR4, CCR6 and CXCR3 (Figure A and B) . Whereas no effects of treatment were found on Th1 and Th17 cells, numbers of Th2 cells were significantly different after treatment.…”

Section: Resultsmentioning

confidence: 99%

“…Regulatory T cells were studied based on the phenotype CD4+ CD25+ CD127− (Figure A) . Numbers of total Tregs decreased slightly but not significantly after treatment (Figure B).…”

Section: Resultsmentioning

confidence: 99%

“…Flow cytometric analyses were performed on a 4‐laser LSRFortessa (BD Biosciences) using standardized measurement settings, and data were analysed using FacsDiva V8.0 (BD Biosciences). Immunophenotypic definitions of lymphocyte subsets are provided in Table . The absolute lymphocyte counts obtained from fresh samples were used to calculate absolute numbers of the CD3+ T cell and CD19+ B cell subsets.…”

Section: Methodsmentioning

confidence: 99%

“…Immunophenotypic definitions of lymphocyte subsets are provided in Table S2. 33,44,45 The absolute lymphocyte counts obtained from fresh samples were used to calculate absolute numbers of the CD3+ T cell and CD19+ B cell subsets.…”

Section: Clinical Outcome Measuresmentioning

confidence: 99%

“…Th1, Th2 and Th17 cells were defined within the CD45RA-memory compartment based on differential expression of the chemokine receptors CCR4, CCR6 and CXCR3 (Figure 2A and B). 44 (Figure 2A). As a result, the treatments also had significantly different effects on the Th1/Th2 cell ratios between the WKZ and the Davos group (P < .05) (Figure 2A).…”

Section: Reduction In Eosinophils and Increase In T Cells After Alpmentioning

confidence: 99%

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Treatment for moderate to severe atopic dermatitis in alpine and moderate maritime climates differentially affects helper T cells and memory B cells in children

Heeringa

Fieten

Bruins

et al. 2018

Clin Experimental Allergy

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show abstract

Section: Resultsmentioning

confidence: 99%

“…Regulatory T cells were studied based on the phenotype CD4+ CD25+ CD127− (Figure A) . Numbers of total Tregs decreased slightly but not significantly after treatment (Figure B).…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Clinical Outcome Measuresmentioning

confidence: 99%

Section: Reduction In Eosinophils and Increase In T Cells After Alpmentioning

confidence: 99%

See 3 more Smart Citations

Treatment for moderate to severe atopic dermatitis in alpine and moderate maritime climates differentially affects helper T cells and memory B cells in children

Heeringa

Fieten

Bruins

et al. 2018

Clin Experimental Allergy

View full text Add to dashboard Cite

show abstract

Patterns of T‐Cell Phenotypes in Rheumatic Diseases From Single‐Cell Studies of Tissue

Gao

Dunlap

Elahee

et al. 2021

ACR Open Rheumatology

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High‐dimensional analyses of tissue samples from patients with rheumatic diseases are providing increasingly detailed descriptions of the immune cell populations that infiltrate tissues in different rheumatic diseases. Here we review key observations emerging from high‐dimensional analyses of T cells within tissues in different rheumatic diseases, highlighting common themes across diseases as well as distinguishing features. Single‐cell RNA sequencing analyses capture several dimensions of T‐cell states, yet surprisingly, these analyses generally have not demonstrated distinct clusters of paradigmatic T‐cell effector subsets, such as T helper (Th) 1, Th2, and Th17 cells. Rather, global transcriptomics robustly identify both proliferating T cells and regulatory T cells and have also helped to reveal new effector subsets in inflamed tissues, including T peripheral helper cells and granzyme K+ T cells. Further characterization of the T‐cell populations that accumulate within target tissues should enable more precise targeting of biologic therapies and accelerate development of more specific biomarkers to track activity of relevant immune pathways in patients with rheumatic diseases.

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Defective formation of IgA memory B cells, Th1 and Th17 cells in symptomatic patients with selective IgA deficiency

et al. 2020

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Objective. Selective IgA deficiency (sIgAD) is the most common primary immunodeficiency in Western countries. Patients can suffer from recurrent infections and autoimmune diseases because of a largely unknown aetiology. To increase insights into the pathophysiology of the disease, we studied memory B and T cells and cytokine concentrations in peripheral blood. Methods. We analysed 30 sIgAD patients (12 children, 18 adults) through detailed phenotyping of peripheral B-cell, CD8 + T-cell and CD4 + Tcell subsets, sequence analysis of IGA and IGG transcripts, in vitro B-cell activation and blood cytokine measurements. Results. All patients had significantly decreased numbers of T-cell-dependent (TD; CD27 + ) and T-cell-independent (TI; CD27 À ) IgA memory B cells and increased CD21 low B-cell numbers. IgM + IgD À memory B cells were decreased in children and normal in adult patients. IGA and IGG transcripts contained normal SHM levels. In sIgAD children, IGA transcripts more frequently used IGA2 than controls (58.5% vs. 25.1%), but not in adult patients. B-cell activation after in vitro stimulation was normal. However, adult sIgAD patients exhibited increased blood levels of TGF-b1, BAFF and APRIL, whereas they had decreased Th1 and Th17 cell numbers. Conclusion. Impaired IgA memory formation in sIgAD patients is not due to a B-cell activation defect. Instead, decreased Th1 and Th17 cell numbers and high blood levels of BAFF, APRIL and TGF-b1 might reflect disturbed regulation of IgA responses in vivo.These insights into B-cell extrinsic immune defects suggest the need for a broader ª immunological focus on genomics and functional analyses to unravel the pathogenesis of sIgAD.

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Expansion of blood IgG 4 + B, T H 2, and regulatory T cells in patients with IgG 4 -related disease

Cited by 71 publications

References 75 publications

Treatment for moderate to severe atopic dermatitis in alpine and moderate maritime climates differentially affects helper T cells and memory B cells in children

Treatment for moderate to severe atopic dermatitis in alpine and moderate maritime climates differentially affects helper T cells and memory B cells in children

Patterns of T‐Cell Phenotypes in Rheumatic Diseases From Single‐Cell Studies of Tissue

Defective formation of IgA memory B cells, Th1 and Th17 cells in symptomatic patients with selective IgA deficiency

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